Characterization of an allotriploid strawberry Fragaria × bifera Duchesne (Rosaceae) from Europe


  • Houshang Nosrati Department of Plant Science, University of Tabriz, 29-Bahman Blvd., Tabriz
  • Adam H. Price Department of Plant and Soil Science, Cruickshank Building, University of Aberdeen, Aberdeen, AB24 3UU
  • Pedro Gerstberger Bayreuth Centre of Ecology and Environmental Research, Bayreuth
  • Chris C. Wilcock Department of Plant and Soil Science, Cruickshank Building, University of Aberdeen, Aberdeen, AB24 3UU



allopolyploidy, allotripolyploid strawberry, Fragaria, Fragaria × bifera, strawberry genome content


Allopolyploidy has played an important role in the plant evolution. To assess its role in speciation, it is necessary to examine fertility and crossability of hybrids. A hybrid clone of the genus Fragaria with different and complex morphology compared to F. vesca, F. viridis and F. moschata, was detected in Germany (in Bayreuth, Bavaria). The genome size of these plants was measured using flow cytometry and their fertility was tested in experimental crossing. The parental origin of the hybrid was revealed using RAPD approach. From the mean intensity of fluorescence emitted by PI-stained nuclei for F. moschata, F. vesca, F. viridis and the hybrid, triploidy of the hybrid could be indicated. The hybrid shared an 1800bp and 880bp long species-specific RAPDs bands with F. viridis and F. vesca, respectively, indicating them as the parental species of the hybrid. The hybrid did not produce any fruit in selfing, open pollination and when crossed by pollen of F. vesca and F. viridis, all showing female sterility of the hybrid. The hybrid had 78% pollen sterility, however, pollinating F. vesca by pollen of the hybrid produced viable seed and F1 plants, indicating its male fertility. This work shows allopolyploidy role in the evolution and speciation of Fragaria, and may suggest the study site as potential new centre of Fragaria speciation.


Bretagnolle F., Thompson J.D. (1995): Tansley review no. 78. Gametes with the somatic chromosome number: mechanisms of their formation and role in the evolution of atotpolyploid plants. New Phyto. 129: 1-22. DOI:

Bringhurst R.S., Gill T. (1970): Origin of Fragaria polyploids II. Unreduced and doubled–unreduced gametes. Am. J. Bot. 578: 969-976. DOI:

Bringhurst R.S., Senanayake Y.D.A. (1966): The evolutionary significance of natural Fragaria chiloensis x F. vesca hybrids resulting from unreduced gametes. Am. J. Bot. 53: 1000-1006. DOI:

Dickson E.E., Arumuganathan K., Kresovich S., Doyle J.J. (1992): Nuclear DNA content variation within the Rosaceae. Am. J. Bot. 79: 1081-1086. DOI:

Dolezel J., Sgorbati S., Lucretti S. (1992): Comparison of three DNA fluorochromes for flow cytometric estimation of nuclear DNA content in plants. Physio. Planta. 85: 625-631. DOI:

Ellis J.R. (1962): Fragaria–Potentilla intergeneric hybridisation and evolution in Fragaria. Proc. Linn. Soc. London 173: 99-106. DOI:

Fedorova N.J. (1934): Polyploid interspecific hybrids in the genus Fragaraia. Genetica 16: 524-541. DOI:

Galbraith D.W., Harkins K. R., Maddox J. M., Ayres N. M., Sharma D.P., Firoozabady E. (1983): Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220: 1049-1051. DOI:

Grant V. (1981): Plant Speciation, 2nd ed. Columbia University Press, New York, 260 pp. DOI:

Hahn S. K., Bai K. V., Asiedu R. (1990): Tetraploids triploids and 2n pollen from diploid interspecific crosses with cassava. Theo. Appl. Genet. 79: 433-39. DOI:

Harlan J.R., deWet J.M.J. (1975): On a wing and a prayer: the origins of polyploidy. Bot. Rev. 41: 361-90. DOI:

Hummer K.E., Nathewet P., Yanagi T. (2009): Decaploidy in Fragaria iturupensis Rosaceae. Am. J. Bot. 96: 713-716. DOI:

Ichijima K. (1930): Studies on the genetics of Fragaria. Z. Indukt. Abstamm. Vererb. 55: 300-347 [In German, Translated by British Library]. DOI:

Jones H.A., Clarke A.E. (1942): A natural amphidiploid from an onion species hybrid Allium cepa L. × Allium fistulosum L. Heredity 33:25-32. DOI:

Lippert W. (1985): Morphologische und zytologische Unterssuchung an Fragaria insbesondere an Fragaria moschata in Bayern. Bot. Jahrb. Syst. Pflanzengesch. Pflanzengeogr. Stuttgart W. Ger., E. Schweizerbart'sche Verlagsbuchhandlung 107: 195-202.

Lundberg M. (2011): Systematics and polyploid evolution in Potentilleae Rosaceae. PhD thesis, Stockholm University, Stockholm, Sweden, 28 pp.

Nosrati H., Price A.H., Gerstberger P., Wilcock C.C. (2011a): Identification of a natural allopentaploid hybrid Fragaria (Rosaceae) new to Europe. New J. Bot. 1(2): 88-92. DOI:

Nosrati H., Price A.H., Wilcock C.C. (2011b): Relationship between genetic distances and postzygotic reproductive isolation in diploid Fragaria (Rosaceae). Biol. J. Linn. Soc. 104: 510-526. DOI:

Nosrati H., Price A.H., Gerstberger P., Wilcock C.C. (2013): The first report of an alloheptaploid from the genus Fragaria (Rosaceae). New J. Bot. 3(3): 205-209. DOI:

Potter D., Luby J.J., Harrison R.E. (2000): Phylogenetic relationships among species of Fragaria Rosaceae inferred from non-coding nuclear and chloroplast DNA sequences. Syst. Bot. 25: 337-348. DOI:

Ramsey J., Schemske D.W. (1998): Pathways mechanisms and rates of polyploidy formation in flowering plants. Ann. Rev. Ecol. Syst. 29: 467-501. DOI:

Rieseberg L.H. (1997): Hybrid origins of plant species. Ann. Rev. Ecol. Syst. 28: 359-89. DOI:

Scott D.H. (1951): Cytological studies on polyploids derived from tetraploid Fragaria vesca and cultivated strawberries. Genetics 36: 311–331. DOI:

Soltis D.E., Soltis P.S. (1993): Molecular data and the dynamic nature of polyploidy. Crit. Rev. Plant Sci. 12: 243-275. DOI:

Staudt G. (1962): Taxonomic studies in the genus Fragaria, Typification of Fragaria species known at the time of Linnaeus. Can. J. Bot. 40: 869-886. DOI:

Staudt G. (1989): The species of Fragaria, their taxonomy and geographical distribution. Act. Horticul. 265: 23-33. DOI:

Staudt G. (2009): Strawberry biogeography, genetics and systematics. In: López-Medina J. (Ed.), Proceedings of VI International symposium on Strawberry biogeography genetics and systematic, 31 August 2009, Huelva, Spain. ISHS J. Acta Hort., p. 842. DOI:

Staudt G., Dimeglio L.M., Davis T.M., Gerstberger P. (2003): Fragaria × bifera Duch.: Origin and taxonomy. Botanis. Jahr. Syst. Pflanz. Pflanzengeo. 125: 53-72. DOI:

Storey W.B. (1956): Diploid and polyploid gamete formation in orchids. Proc. Amer. Soc. Hort. Sci. 68: 491-502.

Tutin T.G., Heywood V.H., Burges N.A., Moore D.M., Valentine D.H., Walters S.M., Webb D.A. (1968): Flora Europaea. Volume II. Cambridge University Press, Cambridge, 486 pp.

Yokoya K., Roberts A. V., Mottley J., Lewis R., Brandham P.E. (2000): Nuclear DNA amount in Roses. Ann. Bot. 85: 557-561. DOI:






Scientific paper

How to Cite

Nosrati, H., H. Price, A., Gerstberger, P., & C. Wilcock, C. (2015). Characterization of an allotriploid strawberry Fragaria × bifera Duchesne (Rosaceae) from Europe. Natura Sloveniae, 17(1), 5-15.