Characterization of an allotriploid strawberry Fragaria × bifera Duchesne (Rosaceae) from Europe


  • Houshang Nosrati Department of Plant Science, University of Tabriz, 29-Bahman Blvd., Tabriz
  • Adam H. Price Department of Plant and Soil Science, Cruickshank Building, University of Aberdeen, Aberdeen, AB24 3UU
  • Pedro Gerstberger Bayreuth Centre of Ecology and Environmental Research, Bayreuth
  • Chris C. Wilcock Department of Plant and Soil Science, Cruickshank Building, University of Aberdeen, Aberdeen, AB24 3UU



allopolyploidy, allotripolyploid strawberry, Fragaria, Fragaria × bifera, strawberry genome content


Allopolyploidy has played an important role in the plant evolution. To assess its role in speciation, it is necessary to examine fertility and crossability of hybrids. A hybrid clone of the genus Fragaria with different and complex morphology compared to F. vesca, F. viridis and F. moschata, was detected in Germany (in Bayreuth, Bavaria). The genome size of these plants was measured using flow cytometry and their fertility was tested in experimental crossing. The parental origin of the hybrid was revealed using RAPD approach. From the mean intensity of fluorescence emitted by PI-stained nuclei for F. moschata, F. vesca, F. viridis and the hybrid, triploidy of the hybrid could be indicated. The hybrid shared an 1800bp and 880bp long species-specific RAPDs bands with F. viridis and F. vesca, respectively, indicating them as the parental species of the hybrid. The hybrid did not produce any fruit in selfing, open pollination and when crossed by pollen of F. vesca and F. viridis, all showing female sterility of the hybrid. The hybrid had 78% pollen sterility, however, pollinating F. vesca by pollen of the hybrid produced viable seed and F1 plants, indicating its male fertility. This work shows allopolyploidy role in the evolution and speciation of Fragaria, and may suggest the study site as potential new centre of Fragaria speciation.


Bretagnolle F, Thompson JD. 1995. Tansley review no. 78. Gametes with the somatic chromosome number: mechanisms of their formation and role in the evolution of atotpolyploid plants. New Phytologist. 129: 1-22.

Bringhurst RS, Gill T. 1970. Origin of Fragaria polyploids II. Unreduced and doubled–unreduced gametes. American Journal of Botany. 578: 969-976.

Bringhurst RS, Senanayake YDA. 1966. The evolutionary significance of natural Fragaria chiloensis x F. vesca hybrids resulting from unreduced gametes. American Journal of Botany. 53: 1000-1006.

Dickson EE, Arumuganathan K, Kresovich S, Doyle JJ. 1992. Nuclear DNA content variation within the Rosaceae. American Journal of Botany. 79: 1081-1086.

Dolezel J, Sgorbati S, Lucretti S. 1992. Comparison of three DNA fluorochromes for flow cytometric estimation of nuclear DNA content in plants. Physiologia Plantarum. 85: 625-631.

Ellis JR. 1962. Fragaria–Potentilla intergeneric hybridisation and evolution in Fragaria. Proceedings Linnean Society London. 173: 99-106.

Fedorova NJ. 1934. Polyploid interspecific hybrids in the genus Fragaraia. Genetica. 16: 524-541.

Galbraith DW, Harkins KR, Maddox JM, Ayres NM, Sharma DP, Firoozabady E. 1983. Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science. 220: 1049-1051.

Grant V. 1981. Plant Speciation, 2nd ed. New York: Columbia University Press. p. 260.

Hahn SK, Bai KV, Asiedu R. 1990. Tetraploids triploids and 2n pollen from diploid interspecific crosses with cassava. Theoretical and Applied Genetics. 79: 433-39.

Harlan JR, deWet JMJ. 1975. On a wing and a prayer: the origins of polyploidy. The Botanical Review. 41: 361-90.

Hummer KE, Nathewet P, Yanagi T. 2009. Decaploidy in Fragaria iturupensis Rosaceae. American Journal of Botany. 96: 713-716.

Ichijima K. 1930. Studies on the genetics of Fragaria. British Library, translator. Zeitschrift fur induktive Abstammungs-und Vererbungslehre. 55: 300-347.

Jones HA, Clarke AE. 1942. A natural amphidiploid from an onion species hybrid Allium cepa L. × Allium fistulosum L. Journal of Heredity. 33:25-32.

Lippert W. 1985. Morphologische und zytologische Unterssuchung an Fragaria insbesondere an Fragaria moschata in Bayern. Botanische Jahrbucher fur Systematik, Pflanzengeschichte und Pflanzengeographie. Stuttgart: E. Schweizerbart'sche Verlagsbuchhandlung. 107: 195-202.

Lundberg M. 2011. Systematics and polyploid evolution in Potentilleae Rosaceae [PhD dissertation]. Stockholm: Stockholm University, Stockholm, Sweden. p. 28.

Nosrati H, Price AH, Gerstberger P, Wilcock CC. 2011a. Identification of a natural allopentaploid hybrid Fragaria (Rosaceae) new to Europe. New Journal of Botany. 1(2): 88-92.

Nosrati H, Price AH, Wilcock CC. 2011b. Relationship between genetic distances and postzygotic reproductive isolation in diploid Fragaria (Rosaceae). Biological Journal of the Linnean Society. 104: 510-526.

Nosrati H, Price AH, Gerstberger P, Wilcock CC. 2013. The first report of an alloheptaploid from the genus Fragaria (Rosaceae). New Journal of Botany. 3(3): 205-209.

Potter D, Luby JJ, Harrison RE. 2000. Phylogenetic relationships among species of Fragaria Rosaceae inferred from non-coding nuclear and chloroplast DNA sequences. Systematic Botany. 25: 337-348.

Ramsey J, Schemske DW. 1998. Pathways mechanisms and rates of polyploidy formation in flowering plants. Annual Review of Ecology and Systematics. 29: 467-501.

Rieseberg LH. 1997. Hybrid origins of plant species. Annual Review of Ecology and Systematics. 28: 359-89.

Scott DH. 1951. Cytological studies on polyploids derived from tetraploid Fragaria vesca and cultivated strawberries. Genetics. 36: 311–331.

Soltis DE, Soltis PS. 1993. Molecular data and the dynamic nature of polyploidy. Critical Reviews in Plant Sciences. 12: 243-275.

Staudt G. 1962. Taxonomic studies in the genus Fragaria, Typification of Fragaria species known at the time of Linnaeus. Canadian Journal of Botany. 40: 869-886.

Staudt G. 1989. The species of Fragaria, their taxonomy and geographical distribution. Acta Horticulturae. 265: 23-33.

Staudt G. 2009. Strawberry biogeography, genetics and systematics. López-Medina J, editors. Proceedings of VI International symposium on Strawberry biogeography genetics and systematic, 31 August 2009, Huelva, Spain. ISHS Acta Horticulturae. p. 842.

Staudt G, Dimeglio LM, Davis TM, Gerstberger P. 2003. Fragaria × bifera Duch.: Origin and taxonomy. Botanische Jahrbücher für Systematik, Pflanzengeschichte und Pflanzengeographie. 125: 53-72.

Storey WB. 1956. Diploid and polyploid gamete formation in orchids. Proceedings. American Society for Horticultural Science. 68: 491-502.

Tutin TG, Heywood VH, Burges NA, Moore DM, Valentine DH, Walters SM, Webb DA. 1968. Flora Europaea. Volume II. Cambridge: Cambridge University Press. p. 486.

Yokoya K, Roberts AV, Mottley J, Lewis R, Brandham PE. 2000. Nuclear DNA amount in Roses. Annals of Botany. 85: 557-561.






Scientific paper

How to Cite

Nosrati, H., H. Price, A., Gerstberger, P., & C. Wilcock, C. (2015). Characterization of an allotriploid strawberry Fragaria × bifera Duchesne (Rosaceae) from Europe. Natura Sloveniae, 17(1), 5-15.