The ichthyofauna of the upper Neretva River


  • Jakob Neuburg University of Natural Resources and Life Sciences, Institute of Hydrobiology and Aquatic Ecosystem Management, Gregor-Mendel-Straße 33, 1180 Vienna
  • Thomas Friedrich University of Natural Resources and Life Sciences, Institute of Hydrobiology and Aquatic Ecosystem Management, Gregor-Mendel-Straße 33, 1180 Vienna
  • Elisabeth Haring Natural History Museum Vienna, Maria-Theresien-Platz 1010 Vienna
  • Sarah Höfler Blattfisch e.U. – Consultants in Aquatic Ecology and Engineering, Leopold-Spitzer-Straße 26, 4600 Wels
  • Ana Maric University of Belgrade, Faculty of Biology, Studentski treg 16, 11000 Belgrade
  • Saša Marić University of Belgrade, Faculty of Biology, Studentski trg 16, 11000 Belgrade
  • Stephanie Popp University of Natural Resources and Life Sciences, Institute of Hydrobiology and Aquatic Ecosystem Management, Gregor-Mendel-Straße 33, 1180 Vienna
  • Predrag Simonović University of Belgrade, Faculty of Biology, Studentski trg 16, 11000 Belgrade
  • Aleš Snoj University of Ljubljana, Department of Animal Science, Biotechnical Faculty, Jamnikarjeva 101, 1000 Ljubljana
  • Simona Sušnik Bajec University of Ljubljana, Department of Animal Science, Biotechnical Faculty, Jamnikarjeva 101, 1000 Ljubljana
  • Simon Vitecek University of Natural Resources and Life Sciences, Institute of Hydrobiology and Aquatic Ecosystem Management, Gregor-Mendel-Straße 33, 1180 Vienna
  • Alexandra Wanka Natural History Museum Vienna, Maria-Theresien-Platz 1010 Vienna
  • Steven Weiss University of Graz, Faculty of Natural Sciences Universitätsplatz 2, 8010 Graz
  • Kurt Pinter University of Natural Resources and Life Sciences, Institute of Hydrobiology and Aquatic Ecosystem Management, Gregor-Mendel-Straße 33, 1180 Vienna



fish stock assessment, electrofishing, snorkelling, eDNA, genetics, hydropower, Salmo obtusirostris


Fish were sampled at nine locations of the upper Neretva River, upstream of Glavatičevo. Evidence of the endangered softmouth trout (Salmo obtusirostris) was obtained as well as the presence of other native and non-native salmonids. Genetic analysis suggests that the native brown trout (Salmo trutta) in the upper Neretva is homogeneous with no significant introgression by introduced Atlantic-lineage brown trout and quantitative catch data show that their populations are healthy with densities above 1,000 ind/ha. However, the relatively low biomass estimates may be the result of overharvesting. A new haplotype of the mitochondrial control region of the bullhead (Cottus gobio) was detected and confirmation of native S. trutta, Barbatula barbatula, Phoxinus sp., and C. gobio in the upper reaches, underlining the importance of this river stretch. The planned expansion of hydropower plants in the upper Neretva and its tributaries will have a significant negative impact on the largely untouched ichthyofauna, as is clearly demonstrated by the conditions found in the middle course of the river, which has been dedicated to hydropower production.


Bartulović V, Glamuzina B, Conides A, Dulcic J, Lucic D, Njire, J, Kozul V. 2004. Age, growth, mortality and sex ratio of sand smelt, Atherina boyeri Risso, 1810 (Pisces: Atherinidae) in the estuary of the Mala Neretva River (middle-eastern Adriatic, Croatia). Journal of Applied Ichthyology. 20(5): 427–430. DOI:

Belkhir K, Borsa P, Chikhi L, Raufaste N, Bonhomme F. 1996-2004. GENETIX v. 4.04, Logiciel sous WindowsTM pour la génétique des populations. Montpellier: Université Montpellier 2, Laboratoire Génome et Population.

Benzécri JP, Benzécri F, Birou A, Blumenthal S. 1973. L’analyse des données. Volume 2: L’analyse des correspondances. Paris: Dunod.

Bernatchez L, Danzmann RG.1993. Congruence in control-region sequence and restriction-site variation in mitochondrial DNA of brook charr (Salvelinus fontinalis Mitchill). Molecular Biology and Evolution. 10: 1002–1014.

Bernatchez L, Guyomard R, Bonhomme F. 1992. DNA sequence variation of the mitochondrial control region among geographically and morphologically remote European brown trout Salmo trutta populations. Molecular Ecology. 1: 161-173. DOI:

Berrebi P, Horvath Á, Splendiani A, Palm S, Bernaś R. 2021. Genetic diversity of domestic brown trout stocks in Europe. Aquaculture. 544: 737043. DOI:

Bravničar J. 2012. Taxonomic analysis of bullhead (Cottus sp.) in Slovenia with application of molecular methods [bachelor's thesis]. [Ljubljana]: Univerza u Ljubljani, Biotehniška Fakulteta, Oddelek za Biologijo.

Bravničar J, Palandačić A, Jelić D, Podgornik S, Snoj A. 2021. Molecular data reveal distinct phylogenetic position of Cottus metae, establish its distribution range, and invalidate the species status of C. scaturigo. Journal of Zoological Systematics and Evolutionary Research. 59(2): 428–441. DOI:

Bravničar J, Palandačić A, Marić S, Šanda R, Snoj A. 2015. Phylogeny of European bullhead (Cottus sp.) in Western Balkan. In: Abstract book. XV European Congress of Ichthyology; 7-11 September; Porto. p. 35-36.

Crivelli AJ. 2006. Salmo obtusirostris. The IUCN Red List of Threatened Species 2006: e.T19862A9056634; [accessed on 21.8.2023]. DOI:

DeLury DB. 1947. On the estimation of biological populations. Biometrics. 3(4): 145. DOI:

DIKTAS B&H. 2012. DIKTAS - Protection and sustainable use of the Dinaric Karst transboundary aquifer system. National Report Bosnia and Herzegovina.

Dulčić J, Dragičević B, Ugarković PTP. 2017. The largemouth black bass (Micropterus salmoides): first record in the Neretva River delta, Adriatic drainage system of Croatia. Cybium. 41(1): 77–78.

EN 14011:2003. Water quality-sampling of fish with electricity.

Freyhof J, Brooks E. 2011. European Red List of freshwater fishes. Luxembourg: Publications Office of the European Union.

Glamuzina B, Bartulović V, Glamuzina L, Dobroslavić T. 2017. Records of new fish species in the River Neretva estuary: potential threat to Coastal Adriatic nursery. Naše More. 64(3): 86–89. DOI:

Glamuzina B, Stanić-Koštroman S, Matić-Skoko S, Glamuzina L, Muhamedagić S, Rozić I, Weiss S, Pavličević J. 2018. Recent status and life history traits of endangered soft-mouth trout, Salmo obtusirostris in the River Neretva catchment (Bosnia and Herzegovina) as a consequence of river alteration. Journal of Applied Ichthyology. 34(5): 1160–1168. DOI:

Glamuzina L, Dobroslavić T. 2020. Summer fish migrations in the River Neretva (South-Eastern Adriatic Coast, Croatia) as a consequence of salinization. Naše More. 67(2): 103–116. DOI:

Goudet J. 1995. FSTAT (Version 1.2): a computer program to calculate F-statistics. Journal of Heredity. 86: 485-486. DOI:

Haunschmid R, Schotzko N, Petz-Glechner R, Honsig-Erlenburg W, Schmutz S, Spindler T, Unfer, G, Wolfram G, Bammer V, Hundritsch L, et al. 2010. Leitfaden zur Erhebung der biologischen Qualitätselemente Teil A1 - Fische. Wien: Bundesministerium für Land- und Forstwirtschaft, Umwelt und Wasserwirtschaft.

Hoang DT, Chernomor O, von Haeseler A, Minh BQ, Vinh LS, Rosenberg MS. 2017. UFBoot2: Improving the ultrafast bootstrap approximation. Molecular Biology and Evolution. 35(2): 518–522. DOI:

Huet M. 1949. Aperçu des relations entre la pente et les populations piscicoles des eaux courantes. Zeitschrift Für Hydrologie. 11: 333–351. DOI:

Ivanković P, Treer T, Piria M, Knezović Z. 2010. Diet and growth of the endemic Neretva chub, Squalius svallize from the Neretva river area, Bosnia and Herzegovina. Folia Zoologica. 59(1): 51–56. DOI:

Jakovlić I, Qing Jiang W, Treer T, Šprem N, Jian Fang Gui JF. 2013. Introgression evidence and phylogenetic relationships among three (Para)Misgurnus species as revealed by mitochondrial and nuclear DNA markers. Archives of Biological Sciences. 65(4): 1463-1467. DOI:

Kalamujić B, Škrijelj R, Pojskić N. 2015. Genetic diversity of contemporary populations of Salmo trutta L. in the basin of the river Neretva, Bosnia and Herzegovina. Frontiers in Marine Science. Conference Abstract: XV European Congress of Ichthyology. DOI:

Kalyaanamoorthy S, Minh BQ, Wong TKF, Von Haeseler A, Jermiin LS. 2017. ModelFinder: Fast model selection for accurate phylogenetic estimates. Nature Methods. 14(6): 587–589. DOI:

Katoh K, Standley DM. 2013. MAFFT multiple sequence alignment software version 7: Improvements in performance and usability. Molecular Biology and Evolution. 30(4): 772–780. DOI:

Keppel G, Van Niel KP, Wardell-Johnson GW, Yates CJ, Byrne M, Mucina L, Schut AGT, Hopper SD, Franklin SE. 2012. Refugia: Identifying and understanding safe havens for biodiversity under climate change. Global Ecology and Biogeography. 21( 4): 393–404. DOI:

Lerceteau-Köhler E, Weiss S. 2006. Development of multiplex PCR microsatellite assay in brown trout Salmo trutta, and its potential application for the genus. Aquaculture. 258: 641-645. DOI:

Marić S, Stanković D, Sušnik Bajec S, Vukić J, Šanda R, Stefanov T, Nikolić D, Snoj A. 2022. Perils of brown trout (Salmo spp.) mitigation driven translocations: a case study from the Vlasina Plateau, Southeast Serbia. Biological Invasions. 24: 999-1016. DOI:

Minh BQ, Schmidt HA, Chernomor O, Schrempf D, Woodhams MD, Von Haeseler A, Lanfear R, Teeling E. 2020. IQ-TREE 2: New models and efficient methods for phylogenetic inference in the genomic era. Molecular Biology and Evolution. 37(5): 1530–1534. DOI:

Muhamedagić S, Gjoen HM, Vegra M. 2010. Salmonids of the Neretva river basin – present state and suggested sustainable selection programme to protect and strengthen salmonid populations. Proceedings of the EIFAC Symposium on Interactions between Social, Economic and Ecological Objectives of Inland Commercial and Recreational Fisheries and Aquaculture; 21-24 May 2008; Antalya, Turkey.

Muhamedagić S, Pavličević J, Savić N. 2019. Composition of ichthyopopulations in the upper stream of the Neretva River, half a century after the construction of the first dam. Works of the Faculty of Agriculture and Food Sciences, University of Sarajevo, Vol. LXIV, No. 69/1.

Nguyen LT, Schmidt HA, Von Haeseler A, Minh BQ. 2015. IQ-TREE: A fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution. 32(1): 268–274. DOI:

Oikonoumou A, Leprieur F, Leonardos ID. 2014. Biogeography of freshwater fishes of the Balkan Peninsula. Hydrobiologia. 738: 205-220. DOI:

Palandačić A, Bravničar J, Zupančič P, Šanda R, Snoj A. 2015. Molecular data suggest a multispecies complex of Phoxinus (Cyprinidae) in the Western Balkan Peninsula. Molecular Phylogenetics and Evolution. 92: 118–123. DOI:

Palandačić A, Kruckenhauser L, Ahnelt H, Mikschi E. 2020. European minnows through time: museum collections aid genetic assessment of species introductions in freshwater fishes (Cyprinidae: Phoxinus species complex). Heredity. 124(3): 410–422. DOI:

Palandačić A, Naseka A, Ramler D, Ahnelt H. 2017. Contrasting morphology with molecular data: An approach to revision of species complexes based on the example of European Phoxinus (Cyprinidae). BMC Evolutionary Biology. 17(1). DOI:

Pavličević J, Glamuzina L, Conides A, Savić N, Rozić I, Klaoudatos D, Kazić A, Glamuzina B. 2016. Pikeperch, Sander lucioperca invasion in the Neretva River watershed (Bosnia and Herzegovina, Croatia) after alteration of river flow. River Research and Applications. 32(5): 967–974. DOI:

Pilić S, Gajević M, Vesnić A, Šljuka S, Korjenić E, Đug S, Mušović A. 2021. A preliminary study of morphometric characteristics and reproductive potential of the fish species Cottus gobio Linnaeus, 1758 on selected sites from Bosnia and Herzegovina. Veterinaria. 70(3): 293–310. DOI:

Pont D, Rocle M, Valentini A, Civade R, Jean P, Maire A, Roset N, Schabuss M, Zornig H, Dejean T. 2018. Environmental DNA reveals quantitative patterns of fish biodiversity in large rivers despite its downstream transportation. Scientific Reports. 8(10361). DOI:

Razpet A, Sušnik Bajec S, Rozman T, Snoj A. 2007. Genetic variation among trout in the river Neretva basin, Bosnia and Herzegovina. Journal of Fish Biology. 71(sa): 94-110. DOI:

Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning. A laboratory manual. 2nd ed. Cold Spring Harbor Laboratory Press. New York (NY): Cold Spring Harbor.

Schöffmann J, Marić S, Snoj A. 2019. Trout of Southeast Europe, Western and Central Asia. In: Kershner LK, Williams JE, Greswell RE, Lobón-Cerviá J, editors. Trout and char of the world. Bethesda (MD): American Fisheries Society. DOI:

Seber GAF, Cren EDL. 1967. Estimating population parameters from catches large relative to the population. The Journal of Animal Ecology. 36(3): 631. DOI:

Skoulikidis NT, Zogaris S, Karaouzas I. 2009. Rivers of the Balkans. In: Rivers of Europe. [place unknown]: Elsevier. p. 595–655. DOI:

Šlechtová V, Bohlen J, Freyhof J, Persat H, Delmastro GB. 2004. The Alps as barrier to dispersal in cold-adapted freshwater fishes? Phylogeographic history and taxonomic status of the bullhead in the Adriatic freshwater drainage. Molecular Phylogenetics and Evolution. 33(1). 225–239. DOI:

Snoj A, Bogut I, Sušnik S. 2008. Evidence of a genetically distinct population of Vrljika softmouth trout Salmo obtusirostris Heckel evolved by vicariance. Journal of Fish Biology. 72(8). 1945–1959. DOI:

Snoj A, Glamuzina B, Razpet A, Zablocki J, Bogut I, Lerceteau-Köhler E, Pojskuć N, Sušnik Bajec S. 2010. Resolving taxonomic uncertainties using molecular systematics: Salmo dentex and the Balkan trout community. Hydrobiologia. 651(1): 199-212. DOI:

Snoj A, Melkić E, Sušnik Bajec S, Muhamedagić S, Dovć P. 2002. DNA phylogeny supports revised classification of Salmothymus obtusirostris. Biological Journal of the Linnaean Society. 77(3): 399-411. DOI:

Snoj A, Razpet A, Tomljanović T, Treer T, Sušnik S. 2007. Genetic composition of the Jadro softmouth trout following translocation into a new habitat. Conservation Genetics. 8(5): 1213–1217. DOI:

Tamura K, Stecher G, Kumar S. 2021. MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution. 38(7): 3022–3027. DOI:

Thurow RF. 1994. Underwater methods for study of salmonids in the Intermountain West. General Technical Report - US Department of Agriculture, Forest Service. INT-GTR-307. DOI:

Tougard C, Justy F, Guinand B, Douzery EJP, Berrebi P. 2018. Salmo macrostigma (Teleostei, Salmonidae): Nothing more than a brown trout (S. trutta) lineage? Journal of Fish Biology. 93: 302–310. DOI:

Trifinopoulos J, Nguyen LT, von Haeseler A, Minh BQ. 2016. W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Research. 44(W1): W232–W235. DOI:

Tutman P, Buj I, Ćaleta M, Hamzić A, Korjenić E, Adrović A, Glamuzina B. 2017. Status and distribution of spined loaches (Cobitidae) and stone loaches (Nemacheilidae) in Bosnia and Herzegovina. Folia Zoologica. 66(4): 211–226. DOI:

Tutman P, Dragičević B, Dulčić J, Bukvić V, Bekh V, Glamuzina B. 2021. Records of invasive European catfish Silurus glanis (Linnaeus, 1758) in the Neretva River delta (Croatia): Social Media as Information Source. Croatian Journal of Fisheries. 79(1), 47–52. DOI:

Tutman P, Dragičević B, Dulčić J, Glamuzina L, Bukvić V, Vekić J. 2021. Unusual records of marine organisms in the Neretva River (Croatia and Bosnia and Herzegovina). Acta Adriatica. 62(2): 139–148. DOI:

Tutman P, Glamuzina B, Dulčić J, Zovko N. 2012. Ichthyofauna of the Hutovo Blato wetland (lower River Neretva, Bosnia and Herzegovina); status and vulnerability. Croatian Journal of Fisheries. 70(4): 169–185.

Uiblein F, Jagsch A, Honsig-Erlenburg W, Weiss S. 2001. Status, habitat use, and vulnerability of the European grayling in Austrian waters. Journal of Fish Biology. 59: 223-247. DOI:

Unfer G, Pinter K. 2018. Recreational fisheries: The need for sustainability in fisheries management of Alpine rivers. In: Schmutz S, Sendzimir J, editors. Riverine Ecosystem Management. Cham: Springer. p. 253–270. DOI:

Vegara M, Muhamedagić S, Hamzić A. et al. 2009. Mid-term improvement program of fisheries for the fishing area of Kalinovik municipality. Norwegian University of Life Sciences (UMB), NORAGRIC, AAS, Norway, Norwegian SEE programme: Institutional collaboration between academic institutions in agriculture, forestry and veterinary medicine in Norway and Bosnia and Herzegovina, Croatia and Serbia and Montenegro, 2006-2009.

Vukić J, Eliášová K, Marić D, Šanda R. 2019. Occurrence of alien spirlin (Alburnoides sp.) in the Neretva River basin. Knowledge & Management of Aquatic Ecosystems. 420: 15. DOI:

Ward RD, Zemlak TS, Innes BH, Last PR, Hebert PDN. 2005. DNA barcoding Australia’s fish species. Philosophical Transactions of the Royal Society B: Biological Sciences. 360(1462): 1847–1857. DOI:




How to Cite

Neuburg, J., Friedrich, T., Haring, E., Höfler, S., Maric, A., Marić, S., Popp, S., Simonović, P., Snoj, A., Sušnik Bajec, S., Vitecek, S., Wanka, A., Weiss, S., & Pinter, K. (2023). The ichthyofauna of the upper Neretva River. Natura Sloveniae, 25(3), 155-180.

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