Exogenous silicon leads to increased antioxidant capacity in freezing-stressed pistachio leaves

Authors

  • Ghader HABIBI Department of Biology, Payame Noor University, I. R. of Iran

DOI:

https://doi.org/10.14720/aas.2015.105.1.05

Keywords:

pistacia vera, antioxidants, enzymes, oxidation, damage, stress, freezing, fertilizer application, foliar application, leaf area, membranes, moisture content

Abstract

 

 

Freezing stress limits photosynthesis and growth of plants. This may be attributed to the enhancement of freezing-associated oxidative damage. In this study, we followed precisely changes in the extent of lipid peroxidation and oxidative damage in leaves of pistachio (Pistacia vera ‘Ahmadaghaii’) plants exposed to foliar-applied silicon (Si) under freezing stress. The foliar-applied Si decreased significantly damaging effects of cold on relative water content (RWC), accompanied by an increase in shoot fresh mass (SFM). In addition, pre-Si treatment caused a significant reduction of the leaf area lost by freezing. There was a remarkable increase in phenylalanine ammonia-lyase (PAL) activity during recovery. Since leaf phenolic content was not affected by supplementary Si, the possibility that exogenously applied Si directly influences the activity of PAL seems thin. In the present work, freezing stress caused great membrane damage, as assessed by lipid peroxidation, but Si application significantly reduced the membrane damage because of an efficient scavenging by superoxide dismutase (SOD) and peroxidase (POD). Under freezing, despite the increasing POD activity, Si-supplied plants accumulated the highest levels of hydrogen peroxide (H2O2) may act as a signal for recovery ability from freezing injury. A positive correlation was found between the concentration of malondialdehyde (MDA) and the percentage of necrotic leaf area. This study suggests that the possible mechanisms for Si enhanced freezing resistance may be attributed to the higher antioxidant defense activity and lower lipid peroxidation through leaf water retention, in addition to its role as a mere physical barrier.

 

References

Apel K., Hirt, H. 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology, 55: 373-399; DOI: 10.1146/annurev.arplant.55.031903.141701

Bharti A.K., Khurana, J.P. 1997. Mutant of Arabidopsis as tools to understand the regulation of phenylpropanoids pathway and UVB protection mechanism. Journal of Photochemistry and Photobiology, 65: 765-776; DOI: 10.1111/j.1751- 1097.1997.tb01923.x

Boominathan R., Doran P.M. 2002. Ni induced oxidative stress in roots of the Ni hyperaccumlator, Alyssum bertoloni. New phytologist, 156: 202-205; DOI: 10.1046/j.1469-8137.2002.00506.x

Bradford M.M. 1967. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254; DOI: 10.1016/0003-2697(76)90527-3

Broadley M., Brown P., Cakmak I., Ma J.F., Rengel Z., Zhao F.P. 2011. Beneficial elements. In: Marschner's Mineral Nutrition of Higher Plants. Marschner P. (eds.). Academic Press, UK, London: 249-269.

Cooke J., Leishman M.R. 2011. Is plant ecology more siliceous than we realise? Trends in Plant Science, 16: 61-68; DOI: 10.1016/j.tplants.2010.10.003

Dallagnol L.J., Rodrigues F.A., Tanaka F.A.O., Amorim L., Camargo L.E.A. 2012. Effect of potassium silicate on epidemic components of powdery mildew on melon. Plant Pathology, 61: 323-330; DOI: 10.1111/j.1365-3059.2011.02518.x

Ding M., Hou P., Shen X. 2010. Salt-induced expression of genes related to Na+/K+ and ROS homeostasis in leaves of salt-resistant and saltsensitive poplar species. Plant Molecular Biology, 73: 251-269; DOI: 10.1007/s11103-010-9612-9

Garbuzov M., Reidinger S., Hartley S.E. 2011. Interactive effects of plant-available soil silicon and herbivory on competition between two grass species. Annals of Botany, 108: 1355-1363; DOI: 10.1093/aob/mcr230

Guntzer F., Keller C., Meunier J.D. 2011. Benefits of plant silicon for crops: a review. Agronomy for Sustainable Development, 32: 201-213; DOI: 10.1007/s13593-011-0039-8 Habibi G., Hajiboland R. 2013. Alleviation of drought stress by silicon supplementation in pistachio (Pistacia vera L.) plants. Folia Horticulturae, 25: 21-29; DOI: 10.2478/fhort-2013-0003

Habibi G. 2014a. Role of trace elements in alleviating environmental stress. In: Emerging Technologies and Management of Crop Stress Tolerance Biological Techniques. Ahmad P., Rasool S. (eds.). Elsevier, USA: 313-331; DOI: 10.1016/B978-0-12- 800876-8.00014-X

Habibi G. 2014b. Silicon supplementation improves drought tolerance in canola plants. Russian Journal of Plant Physiology, 61, 6: 784-791; DOI: 10.1134/S1021443714060077

Hattori T., Inanaga S., Araki H., An P., Morita S., Luxova M., Lux A. 2005. Application of silicon enhanced drought tolerance in Sorghum bicolor. Physiologia Plantarum, 123: 459-466; DOI: 10.1111/j.1399-3054.2005.00481.x

He Y., Xiao H., Wang H., Chen Y., Yu M. 2010. Effect of silicon on freezing-induced changes of solutes, antioxidants, and membrane stability in seashore paspalum turfgrass. Acta Physiologiae Plantarum, 32: 487-494; DOI: 10.1007/s11738-009-0425-x

Hossain M.T., Soga K., Wakabayashi K., Kamisaka S., Fujii S., Yamamoto R., Hoson T. 2007. Modification of chemical properties of cell walls by silicon and its role in regulation of the cell wall extensibility in oat leaves. Journal of Plant Physiology, 164, 4: 385-393; DOI: 10.1016/j.jplph.2006.02.003

Irigoyen J.J., Juan J.P.D., Diaz M.S. 1996. Drought enhances freezing tolerance in a freezing-sensitive maize (Zea mays). New Phytologist, 134: 53-59; DOI: 10.1111/j.1469-8137.1996.tb01145.x

Jaiswal P.C. 2004. Soil, Plant and Water Analysis, (eds.). New Delhi, Kalyani Publishers.

Kishimoto T., Sekozawa Y., Yamazaki H., Murakawa H., Kuchitsu K., Ishikawa M. 2014. Seasonal changes in ice nucleation activity in blue berry stems and effects of cold treatments in vitro. Environmental and Experimental Botany, 106: 13- 23; DOI: 10.1016/j.envexpbot.2014.02.010

Lara M.V., Disante K.B., Podesta F.E., Andreo C., Drincovich M.F. 2003. Induction of a crassulacean acid like metabolism in the C4 succulent plant, Portulaca oleracea L.: physiological and morphological changes are accompanied by specific modifications in phosphoenolpyruvate carboxylase. Photosynthesis Research, 77: 241-254; DOI: 10.1023/A:1025834120499

Levine A., Tenhaken R., Dixon R., Lamb C. 1994. H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response. Cell 79: 583-593; DOI: 10.1016/0092-8674(94)90544-4

Liang Y., Zhuc J., Li Z., Chua G., Dingc Y., Zhangc J., Sun W. 2008. Role of silicon in enhancing resistance to freezing stress in two contrasting winter wheat cultivars. Environmental and Experimental Botany, 64: 286-294; DOI: 10.1016/j.envexpbot.2008.06.005

Liu P., Yin L., Deng X., Wang S., Tanaka K., Zhang S. 2014. Aquaporin-mediated increase in root hydraulic conductance is involved in siliconinduced improved root water uptake under osmotic stress in Sorghum bicolor L. Journal of Experimental Botany, 65: 4747-4756; DOI: 10.1093/jxb/eru220

Liu J., Lin S., Xu P., Wang X., Bai J. 2009. Effects of exogenous silicon on the activities of antioxidant enzymes and lipid peroxidation in chilling-stressed cucumber leaves. Agricultural Sciences in China, 8, 9: 1075-1086; DOI: 10.1016/S1671- 2927(08)60315-6

Ma J.F. 2004. Role of silicon in enhancing the resistance of plants to biotic and abiotic stresses. Soil Science and Plant Nutrition, 50: 11-18; DOI: 10.1080/00380768.2004.10408447

Marschner, H. 1995. Mineral nutrition of higher plants. Acedemic Press, London, UK.

Miller G., Suzuki N., Ciftci-Yilmaz S., Mittler R. 2010. Reactive oxygen species homeostasis and signaling during drought and salinity stresses. Plant, Cell and Environment, 33: 453-467; DOI: 10.1111/j.1365- 3040.2009.02041.x

Rizwan M., Meunier J.D., Miche H., Keller C. 2012. Effect of silicon on reducing cadmium toxicity in durum wheat (Triticum turgidum L. cv. Claudio W.) grown in a soil with aged contamination. Journal of Hazardous Materials, 209: 326-334; DOI: 10.1016/j.jhazmat.2012.01.033

Schützendübel A., Schwanz P., Teichmann T., Gross K., Langenfeld-Heyser R., Godbold D.L. 2001. Cadmium-induced changes in antioxidative systems, hydrogen peroxide content, and differentiationins cotspine roots. Plant Physiology, 127: 887-898; DOI: 10.1104/pp.010318

Sonobe K., Hattori T., An P., Tsuji W., Eneji A.E., Kobayashi S., Kawamura Y., Tanaka K., Inanaga S. 2011. Effect of silicon application on sorghum root responses to water stress. Journal of Plant Nutrition, 34: 71-82; DOI: 10.1080/01904167.2011.531360

Takahashi D., Li B., Nakayama T., Kawamura Y., Uemura M. 2013. Plant plasma membrane proteomics for improving cold tolerance. Frontiers in Plant Science, 17: 14-90.

Velikova V., Yordanov I., Edreva A. 2000. Oxidative stress and some antioxidant systems in acid raintreated bean plants-protective role of exogenous polyamines. Plant Science, 151: 59-66; DOI: 10.1016/S0168-9452(99)00197-1

Velioglu Y.S., Mazza G., Gao L., Oomah B.D. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural and Food Chemistry, 46: 4113-4117; DOI: 10.1021/jf9801973

Waraich E.A., Amad R., Ashraf M.Y., Ahmad M. 2011. Improving agricultural water use efficiency by nutrient management. Acta agriculturae Scandinavica, 61: 291-304.

Waśkiewicz A., Beszterda M., Goliński P. 2014. Nonenzymatic antioxidants in plants. In: Oxidative damage to plants: Antioxidant networks and signaling. Ahmad P. (eds.). Elsevier, USA, 201- 234; DOI: 10.1016/B978-0-12-799963-0.00007-1

Zhang Q., Zhang J.Z., Chow W.S., Sun L.L., Chen J.W., Chen Y.J., Peng C.L. 2011. The influence of low temperature on photosynthesis and antioxidant enzymes in sensitive banana and tolerant plantain (Musa sp.) cultivars. Photosynthetica, 49: 201-208; DOI: 10.1007/s11099-011-0012-4

Zhu J., Dong C.H., Zhu J.K. 2007. Interplay between cold-responsive gene regulation, metabolism and RNA processing during plant cold acclimation. Current Opinion in Plant Biology, 10: 290-295; DOI: 10.1016/j.pbi.2007.04.010

Zucker M. 1965. Induction of phenylalanine deaminase by light, its relation to chlorogenic acid synthesis in potato tuber tissue. Physiologia Plantarum, 40: 779- 784; DOI: 10.1104/pp.40.5.779

Downloads

Published

26. 11. 2015

Issue

Section

Agronomy section

How to Cite

HABIBI, G. (2015). Exogenous silicon leads to increased antioxidant capacity in freezing-stressed pistachio leaves. Acta Agriculturae Slovenica, 105(1), 43–52. https://doi.org/10.14720/aas.2015.105.1.05

Similar Articles

1-10 of 724

You may also start an advanced similarity search for this article.