Effects of phosphorus fertilizer rate and Pseudomonas fluorescens strain on field pea (Pisum sativum subsp. arvense (L.) Asch.) growth and yield


  • Bahram SALEHI Department of Agronomy and Plant Breeding, Rasht Branch, Islamic Azad University, Rasht, Iran;
  • Hashem AMINPANAH Department of Agronomy and Plant Breeding, Rasht Branch, Islamic Azad University, Rasht, Iran




Pisum sativum, peas, phosphorus, phosphate fertilizers, rhizobacteria, Pseudomonas fluorescens, plant growth, plant growth stimulants, growth, crop yield


A field experiment was conducted at Rezvanshahr, Guilan province, Iran, to evaluate the effects of phosphorus fertilizer rate and Pseudomonas fluorescens strains on growth and yield of field pea (Pisum sativum L.). The experimental design was a randomized complete block in a factorial arrangement with three replicates. Factors were phosphorus fertilizer rates (0, 25, 50, 75, and 100 kg P2O5 ha-1 as triple superphosphate), and seed inoculation with P. florescens strains [control (non-inoculated), inoculated with strain R41, and strain R187). Analysis of variance showed that plant height, seed yield, pod number per m2, 100-seed weight, biological yield, harvest index, and leaf P concentration were significantly influenced by phosphorus fertilizer rate and P. florescens strain. At the same time, phosphorus fertilizer rate × P. fluorescens strain interaction was significant only for 100-seed weight. On the other hand, seed number per pod was significantly affected neither by phosphorus fertilizer rate nor by pseudomonas strains. Result showed that seed yield was significantly increased from 1099 ± 67 to 1898 ± 118 kg ha-1 as P2O5 application rate increased from 0 to 75 kg ha-1, and thereafter relatively remained constant. There was no significant difference in seed yield between plants raised from inoculated seeds with P. fluorescens, strain R187 (1664 ± 97 kg ha-1) and those raised from inoculated seeds with P. fluorescens, strain R41 (1669 ± 104 kg ha-1). At the same time, plants raised from inoculated seeds with P. fluorescens (both strains) produced greater grain yield compared to those raised from uninoculated seeds (1370 ± 80 kg ha-1). Based on the results of this study, P2O5 application at the rate of 75 kg ha-1 and inoculation with pseudomonas bacteria are recommended for obtaining the greatest seed yield in field pea.


Adesemoye A.O., Torbert H.A., Kloepper J.W. 2009. Plant growth-promoting rhizobacteria allow reduced application rates of chemical fertilizers. Microbial Ecology, 58:921–929, doi: 10.1007/s00248-009-9531-y

Ali H., Khan M.A., Randhawa S.A. 2004. Interactive effect of seed inoculation and phosphorus application on growth and yield of chickpea (Cicer arietinum L.). International Journal of Agriculture and Biology, 6, 1: 110–112

Assuero S.G., Mollier A., Pellerin S. 2004. The decrease in growth of pho sphorus-deficient maize leaves is related to a lower cell production. Plant, Cell and Environment, 27: 887–895, doi: 10.1111/j.1365-3040.2004.01194.x

Benhamou N., Belanger R.R., Paulitz T., 1996b. Ultrastructural and cytochemical aspects of the interaction between Pseudomonas fluorescens and Ri T-DNA transformed pea roots: host response to colonization by Pythium ultimum Trow. Planta, 199: 105–117, doi: 10.1007/BF00196887

Benhamou N., Kloepper J.W., Quadt-Hallmann A., Tuzun S., 1996a. Induction of defense-related ultrastructural modifications in pea root tissues inoculated with endophytic bacteria. Plant Physiology, 112: 919–929

Bhattacharyya P.N., Jha D.K. 2012. Plant growth- promoting rhizobacteria (PGPR): emergence in agriculture. World Journal of Microbiology and Biotechnology, 28:1327–1350, doi: 10.1007/s11274-011-0979-9

Chung H., Park M., Madhaiyan M., Seshadri S., Song J., Cho H., Sa T. 2005. Isolation and characterization of phosphate solubilizing bacteria from the rhizosphere of cr op plants of Korea. Soil Biology and Biochemistry, 37: 1970–1974, doi: 10.1016/j.soilbio.2005.02.025

Dashti N., Zhang F., Hynes R., Smith D.L. 1998. Plant growth promoting rhizobacteria accelerate nodulation and increase nitrogen fixation activity by field grown soybean [Glycine max (L.) Merr.] under short season conditions. Plant and Soil, 200:205–213, doi: 10.1023/A:1004358100856

Davison J. 1988. Plant beneficial bacteria. Natural Biotechnology, 6:282–286, doi: 10.1038/nbt0388- 282

De Meyer G., Hofte M. 1997. Salicylic acid produced by the rhizobacterium Pseudomonas aeruginosa 7NSK2 induces resistance to leaf infection by Botrytis cinerea on bean. Phytopathology, 87: 58– 593, doi: 10.1094/PHYTO.1997.87.6.588

Dey R., Pal K.K., Bhatt D.M., Chauhan S.M. 2004. Growth promotion and yield enhancement of peanut (Arachis hypogaea L.) by application of plant growth-promoting rhizobacteria. Microbiological Research, 159, 371–394, doi: 10.1016/j.micres.2004.08.004

Ferreira J.S., Baldani J.I., Baldani V.L.D. 2010. Selecao de bactérias diazotroficas em duas variedades de arroz. Acta Scientiarum Agronomy, 32: 179–185, doi: 10.4025/actasciagron.v32i1.732

Figueiredo M.V.B., Seldin L., Araujo F.F., Mariano R.L.R. 2011. Plant growth promoting rhizobacteria: fundamentals and applications. In: Maheshwari, D.K. (Ed.), Plant Growth and Health romoting Bacteria. Springer-Verlag, Berlin, Heidelberg, pp. 21–42

Food and Agricultural Organization (FAO). 2012. FAOSTAT statistics database [Online]. Available at http:// http://faostat.fao.org

Grimes H.D., Mount M.S. 1984. Influence of Pseudomonas putida on nodulation of Phaseolus vulgaris . Soil Biology and Biochemistry, 16:27–30, doi: 10.1016/0038-0717(84)90121-4

Gulati A., Sharma N., Vyas P., Sood S., Rahi P., Pathania V., Prasad R. 2010. Organic acid production and plant growth promotion as a function of phosphate solubilization by Acinetobacter rhizosphaerae strain BIHB 723 isolated from the cold deserts of the trans- Himalayas. Archive der Microbiology, 192: 975– 983, doi: 10.1007/s00203-010-0615-3

Gyaneshwar P., Kumar G.N., Parekh L.J., Poole P.S. 2002. Role of soil microorganisms in improving P nutrition of plants. Plant and Soil, 245:83–93, doi: 10.1023/A:1020663916259

Hussain N., Khan A.Z., Akbar H., Akhtar S. 2006. Growth factors and yield of maize as influenced by phosphorus and potash fertilization. Sarhad Journal of Agriculture, 22, 4: 579–583

Kavanova ́ M., Lattanzi F.A., Grimoldi A.A., Schnyder H. 2006. Phosphorus De ficiency Decreases Cell Division and Elongation in Grass Leaves. Plant Physiology, 141:766–775, doi: 10.1104/pp.106.079699

Khan M.S., Zaidi A., Wani P.A. 2006. Role of phosphatesolubilizing microorganisms in sustainable agriculture – a review. Agronomy for Sustainable Development, 27: 29–43, doi: 10.1051/agro:2006011

Leinhos V., Nacek O. 1994. Bi osynthesis of auxins by phosphate solubilizing rhizobacteria from wheat (Triticum aestivum ) and rye (Secale cereale ). Microbiology Research, 149: 31–35, doi: 10.1016/S0944-5013(11)80132-1

Li S.X., Li S.Q. 1992. Responses of wheat, hairy vetch and pea to phosphate fertilizer. Acta University Agriculturae Boreali-occidentalia, 20: 74–78

Li S.X., Wang Z.H., Stewart B.A. 2011 Differences of Some Leguminous and Nonleguminous Crops in Utilization of Soil Phosphorus and Responses to Phosphate Fertilizers. Advances in Agronomy, 110: 126-249, doi: 10.1016/B978-0-12-385531- 2.00003-7

Li, S.X., Zhao, B.S. 1990. Th e effect of soil nitrogen supplying capacity on phosphate fertilizer efficiency for some legu me crops and non-legume crops. Soil Fertility, 4: 19–23

Lin C.G., Li Z.P., Zhang Y. H., Zou Q.X. 1964. Study on improvement of P fertilizer effect in the calcareous brown soil areas of Shanxi Province. Chinese Journal of Soil Science, 1: 4–12

Lowry O., Lopez A. 1946. Determination of inorganic phosphate in the presents of labile phosphate esters. Journal of Biological Chemistry, 162: 421-426

Raghothama K.G., Karthikeyan A.S. 2005. Phosphate acquisition. Plant and Soil, 274: 37–49, doi: 10.1007/s11104-004-2005-6

Roy S.K., Rahaman S.M.L., Salahuddin A.B.M. 1995. Effect of Rhizobium inoculation and nitrogen on nodulation, growth and seed yield of gram (Cicer arietinum L.). Indian Journal of Agronomy, 65: 853–7

Saravanakumara D., Vijayakumarc C., Kumarb N., Samiyappan R. 2007. PGPR-induced defense responses in the tea plant against blister blight disease. Crop Protection, 26: 556–565, doi: 10.1016/j.cropro.2006.05.007

SAS. 2004. SAS Institute, version 9.1.3. Cary, NC, USA

Sgroy V., Cassan F., Masciarelli O., Del Papa M.F., Lagares A., Luna V. 2009. Isolation and characterization of endophytic plant growth- promoting (PGPB) or stress homeostasis-regulating (PSHB) bacteria associ ated to the halophyte Prosopis strombulifera. Applied Microbiology and Biotechnology, 85:371–381, doi: 10.1007/s00253- 009-2116-3

Shaharoona, B., Naveed, M., Arshad, M., Zahir, Z.A., 2008. Fertilizer-dependent efficiency of Pseudomonads for improving growth, yield, and nutrient use efficiency of wheat (Triticum aestivum L.). Applied Microbiology and Biotechnology, 79: 147–155, doi: 10.1007/s00253-008-1419-0

Sharma A, Johri B.N., Sharma, AK., Glick B.R. 2003 Plant growth-promoting bacterium Pseudomonas sp. strain GRP3 influences iron acquisition in mung bean (Vigna radiata L. Wilzeck). Soil Biology and Biochemistry, 3: 887–894, doi: 10.1016/S0038- 0717(03)00119-6

Urbano G., Aranda P., Gomez-Villalva E. 2003. Nutritional evaluation of pea (Pisum sativum L.) protein diets after mild hydrothermal treatment and with and without added phytase. Journal of Agricultural and Food Chemistry, 51: 2415–2420, doi: 10.1021/jf0209239

Van Elsas J.D., Van Overbeek L.S., Fouchier R. 1991. A specific marker, pat, for studying the fate of introduced bacteria and their DNA in soil using a combination of detection techniques. Plant and Soil, 138: 49–60, doi: 10.1007/BF00011807



26. 11. 2015



Agronomy section

How to Cite

SALEHI, B., & AMINPANAH, H. (2015). Effects of phosphorus fertilizer rate and Pseudomonas fluorescens strain on field pea (Pisum sativum subsp. arvense (L.) Asch.) growth and yield. Acta Agriculturae Slovenica, 105(2), 213–224. https://doi.org/10.14720/aas.2015.105.2.04

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