Sonication of seeds increase germination performance of sesame under low temperature stress


  • Fariborz SHEKARI Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran
  • Seyyed-Hamid MUSTAFAVI Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran
  • Amin ABBASI Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran



sesame, seed germination, germination, seedlings, growth, seed treatment, ultrasound, enzyme activity, water uptake


A laboratory experiment was conducted to determine the effect of ultrasound (US) exposure time on germination behavior of sesame seeds. All tests were carried out at 20 kHz in a water bath ultrasonic device varying two factors, treatment duration (10, 20 and 30 min) and germination temperature (15, 20 and 25 ºC). Parallel tests were run in which seeds were soaked in water without sonication in order to eliminate the effect of water from US test results. US treatments enhanced seeds water uptake. At mild exposure time it improved sesame seed germination performance and seedling growth at suboptimal temperatures as indicated by higher germination percentage and germination rate. US applying for 20 min had relatively high superoxide dismutase activity; however, had not significant differences with control and US duration for 10 min. The catalase activity was strongly increased by applying the US for a 10 and 20 min. Among the treatments, application of US vibration for 10 and 20 min reduced both of malondialdehyde and H2O2 contents, however high US duration (30 min) increased both of the traits. In general, ultrasonic priming technique can be useful for early planting the sesame seeds, and lead to higher yields.


Aladjadjiyan A. 2002. Study of the influence of magnetic field on some biological characteristics of Zea mays L. Journal of Central European Agriculture 3: 89-94

Aladjadjiyan A. 2007. The Use of Physical Methods for Plant Growing Stimulation in Bulgaria. Journal of Central European Agriculture 8: 369-380

Aladjadjiyan A. 2012. Physical factors for plant growth food quality. In: food production, approaches, challenges and tasks. Intech

Arin L and Kiyak Y. 2003. The effects of pre-sowing treatments on emergence and seedling growth in tomato seed (Lycopersicon esculentum Mill.) under several stress conditions. Pakistan Journal of Biological Science 6: 990–994, doi: 10.3923/pjbs.2003.990.994

Ashraf M and Foolad MR. 2005. Pre-sowing seed treatment: A shotgun approach to improve germination, plant growth and crop yield under saline and non-saline conditions. Advances in Agronomy 88: 223–271, doi: 10.1016/S0065-2113(05)88006-X

Bailly C. 2004. Active oxygen species and antioxidants in seed biology. Seed Science Research 14, 93–107, doi: 10.1079/SSR2004159 Bailly C, Benamar A, Corbineau F, and CÔME D. 1996. Changes in malondialdehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated aging. Physiologia Plantarum 97: 104–110, doi: 0.1111/j.1399- 3054.1996.tb00485.x

Bailly C, Bogatek-Leszczynska R, Côme D, and Corbineau F. 2002. Changes in activities of antioxidant enzymes and lipoxygenase during growth of sunflower seedlings from seeds of different vigour. Seed Science Research 12: 47–55, doi: 10.1079/SSR200197

Bennet M. 2011. Sesame seed: A Handbook for Farmers and Investors. 29/08/11. Available at pdf

Bewley JD and Black M. 1994. Seeds. Physiology of development and germination (2nd edition). New York, Plenum Press

Bochicchio A, Coradeschi MA, Zienna P, Bertolini M, and Vazzana C. 1991. Imbibitional injury in maize seed independent of chilling. Seed Science Research 1: 85- 90, doi: 10.1017/S0960258500000702

Bradford KJ. 2002. Applications of hydrothermal time to quantifying and modeling seed germination and dormancy. Weed Science 50: 248-260, doi: 10.1614/0043- 1745(2002)050[0248:AOHTTQ]2.0.CO;2

Chen Y, Liu Q, Yue Xz, Meng Zw, and Liang L. 2013. Ultrasonic vibration seeds showed improved resistance to cadmium and lead in wheat seedling. Environment science pollution research , DOI 10.1007/S11356-012-1411-1

Crowe J.H., Hoekstra F.A., and Crowe L.M., 1989. Membrane phase transitions are responsible for imbibitional damage in dry pollen. Proceedings of the National Academy of Sciences USA 86: 520–523, doi: 10.1073/pnas.86.2.520

Fateh E, Noroozi H, Farbod M, and Gerami F. 2012. Assessment of Fennel (Foeniculum vulgare) seed germination characteristics as influenced by ultrasonic waves and magnetic water. European Journal of Experimental Biology 2: 662-666

Florez M, Carbonell V, and Martínez E. 2007. Exposure of maize seeds to stationary magnetic fields: Effects of germination and early growth. Environmental and Experimental Botany 59: 68-75, doi: 10.1016/j.envexpbot.2005.10.006

Goussous SJ, Samarah N.H, Alqudah AM, and Othman MO. 2010. Enhancing seed germination of four crop species using an ultrasonic technique. Experimental Agriculture 46: 231–242, doi: 10.1017/S0014479709991062

Hoekstra FA, Crowe JH, and Crowe LM. 1992. Germination and ion leakage are linked with phase transitions of membrane lipids during imbibition of Typha latifoliapollen . Physiologia Plantarum 84: 29– 34, doi: 10.1111/j.1399-3054.1992.tb08760.x

Langham DR. 2007. Phenology of sesame. In: Janick J., and Whipkey A. [ed.], New Crops and New Uses , 210- 275. ASHS Press, Alexandria, VA

Moloi MJ and Westhuizen AJ. 2006. The reactive oxygen species are involved in resistance responses of wheat to the Russian wheat aphid. Journal of Plant Physiology 163: 1118–1125, doi: 10.1016/j.jplph.2005.07.014

Nijsse J, Walther P. and Hoekstra FA. 2004. Cold-induced imbibition damage of lettuce embryos: a study using cryo-scanning electron microscopy. Seed Science Research 14: 117-126, doi: 10.1079/SSR2004161

Olsen LJ and Harada JJ. 1995. Peroxisomes and their assembly in higher plants. Annual Review of Plant Physiology and Plant Molecular Biology 46: 123–146, doi: 10.1146/annurev.pp.46.060195.001011

Predieri S, Norman HA, Krizek DT, Pillai P, Mirccki RM, and Zimmerman RH. 1995. Influence of UV-B radiation on membrane lipid composition and ethylene evolution in 'Doyenne d' Hiver' pear shoots grown in vitro under different photosynthetic photon fluxes. Environmental Experimental Botany 35: 151-160., doi: 10.1016/0098-8472(95)00003-2

Probert R.J. 2000. The role of temperature in the regulation of seed dormancy and germination. In: Fenner M. [ed.] Seeds: The Ecology and Regeneration in Plant Communities . 261–291. CAB International, Wallingford, doi: 10.1079/9780851994321.0261

Puntarulo S, Galleano M, Sanchez RA, and Boveris A. 1991. Superoxide anion and hydrogen peroxide metabolism in soybean embryonic axes during germination. Biochimica et Biophysica Acta 1074: 277–283, doi: 10.1016/0304-4165(91)90164-C

Sharma A, Bhushan JHA, and Dubey RA. 2011. Oxidative stress and antioxidative defense systems in plants growing under abiotic stresses. In: Pessarakli M, [ed.], Handbook of plant and crop stress. 89-138. CRC press

Weiss EA. 2000. Oil Seed Crop. 2nd Edition Blackwell Longman Group Ltd. USA

Yaldagard M, Mortazavi SA, and Tabatabaie F. 2008a. Influence of ultrasonic stimulation on the germination of barley seed and its alpha-amylase activity. African Journal of Biotechnology 7: 2465–2471

Yaldagard M, Mortazavi SA, and Tabatabaie F. 2008b . Application of ultrasonic waves as a priming technique for accelerating and enhancing the germination of barley seed: Optimization of method by the Taguchi approach. Journal of the Institute of Brewing 114: 14–21, doi: 10.1002/j.2050- 0416.2008.tb00300.x

Yeh Y, Chiu MKY, Chen CL, and Sung JM. 2005. Partial vacuum extends the longevity of primed bitter gourd seeds by enhancing their anti-oxidative activities during storage. Science Horticulture 104: 101-112, doi: 10.1016/j.scienta.2004.08.006



26. 11. 2015



Agronomy section

How to Cite

SHEKARI, F., MUSTAFAVI, S.-H., & ABBASI, A. (2015). Sonication of seeds increase germination performance of sesame under low temperature stress. Acta Agriculturae Slovenica, 105(2), 203–212.

Similar Articles

1-10 of 692

You may also start an advanced similarity search for this article.