Sensitivity of field tests, serological and molecular techniques for Plum Pox Virus detection in various tissues
DOI:
https://doi.org/10.14720/aas.2014.103.1.13Keywords:
plum pox potyvirus, elisa, diagnosis, infection, prunus persica, peaches, prunus armeniaca, apricots, prunus angustifolia, plumsAbstract
Sensitivity of field tests (AgriStrip and Immunochromato), DAS-ELISA, two step RT-PCR and real-time RT-PCR for Plum pox virus (PPV) detection was tested in various tissues of apricot, peach, plum and damson plum trees infected with isolates belonging to PPV-D, PPV-M or PPV-Rec, the three strains present in Slovenia. Flowers of apricot and plum in full bloom proved to be a very good source for detection of PPV. PPV could be detected with all tested techniques in symptomatic parts of leaves in May and with one exception even in the beginning of August, but it was not detected in asymptomatic leaves using field tests, DAS-ELISA and partly also molecular techniques. PPV was detected only in some of the samples of asymptomatic parts of the leaves with symptoms and of stalks by field tests and DAS-ELISA. Infections were not detected in buds in August using field tests or DAS-ELISA. Field tests are useful for confirmation of the PPV infection in symptomatic leaves, but in tissues without symptoms DAS-ELISA should be combined or replaced by molecular techniques.References
EPPO 2004. Plum pox potyvirus. EPPO Bulletin. 34 , 247–256, DOI: 10.1111/j.1365-2338.2004.00726.x.
Adams A.N., 1978. The detection of Plum pox virus in Prunus species by enzyme-linked immunosorbent assay (ELISA). Annals of Applied Biology 90: 215-221, DOI: 10.1111/j.1744- 7348.1978.tb02629.x.
Candresse T., M. Cambra, S. Dallot, M. Lanneau, M. Asensio, M.T. Gorris, F. Revers, G. Macquaire, A.Olmos, D. Boscia, J.B. Quiot, J. Dunez, 1998. Comparison of monoclonal antibodies and PCR assays for the typing of isolates belonging to the D and M serotypes of Plum Pox Potyvirus. Phytopathology 88: 198-204, DOI : 10.1094/PHYTO.1998.88.3.198.
Dosba F., M. Lansac, G. Pecheur, B. Teyssier, J.-P. Piquemal, M. Michel, 1986. Plum pox virus detection by ELISA technique in peach and apricot infected at different growing stages. Acta Horticulturae 193: 187-191
Hartmann W. 1999. Breeding of plum cultivars resistant to Plum pox virus . Acta Horticulturae, 484: 487- 490
Hamford G. 1982. The detection of Plum pox virus (PPV) by indicator plants and enzyme-linked immunosorbent assay (ELISA). Acta Horticulturae 130: 151- 159
Kamenova I., S. Milusheva, 2005. Sharka disease in Bulgaria: past, present and future. Biotechnol. & Biotechnol. Eq 19, Special Issue: 22-40 http://www.diagnosisp.com/dp/journals/view_pdf.p hp/?journal_id=1&archive=0&issue_id=8&article_i d=223 (July 2012)
Mavrič Pleško I., M. Viršček Marn, N. Toplak, 2011. Total RNA extraction method and Prunus species infulence the detection of Plum pox potyvirus by real-time RT-PCR. Acta agriculturae Slovenica 97: 105-113.
Myrta A., O. Potere, F. Ismaeil, D. Boscia, 2003. The distribution of Prunus: experiences of diagnosis with ELISA. Options Méditerranéennes. Série B : Etudes et Recherches 45: 107-110
Németh M. 1986. Virus, mycoplasma and rickettsia like diseases of fruit trees. Ak ademia Kiado, Budapest, Hungary, 841 pp.
Neumüller M., W. Hartmann, 2008. The hypersensitivity of european plum (Prunus domestica L.) against the Plum pox virus. Acta Horticulturae 781: 273-279
Polák J. 2008. Characterisati on of different interactions between cultivars of stone fruits and Plum pox virus. Acta Horticulturae 781: 287-293
Szathmáry E., L. Palkovics, 2010. Natural deletion is not unique in the coat protein (CP) of recombinant Plum pox virus (PPV) isolates in Hungary. Julius-Kühn-Archi , 427: 151-155
Wetzel T., T. Candresse, M. Ravelonandro, J. Dunez, 1991. A polymerase chain reaction assay adapted to Plum pox potyvirus detection. Journal of Virological Methods 33: 355-365, DOI: 10.1016/0166-0934(91)90035-X
Downloads
Published
Issue
Section
How to Cite
