Comparative study of diagnostic methods used for monitoring of common grape vine (Vitis vinifera L.) crown gall (Agrobacterium vitis Ophel & Kerr) in Slovenia

Authors

  • Janja LAMOVŠEK Agricultural Institute of Slovenia, Plant Protection Department, Hacquetova ulica 17, SI-1000 Ljubljana, Slovenia
  • Igor ZIDARIČ Agricultural Institute of Slovenia, Plant Protection Department, Hacquetova ulica 17, SI-1000 Ljubljana, Slovenia
  • Irena MAVRIČ PLEŠKO Agricultural Institute of Slovenia, Plant Protection Department, Hacquetova ulica 17, SI-1000 Ljubljana, Slovenia
  • Gregor UREK Agricultural Institute of Slovenia, Plant Protection Department, Hacquetova ulica 17, SI-1000 Ljubljana, Slovenia
  • Stanislav TRDAN University of Ljubljana, Biotechnical Faculty, Dept. of Agronomy, Jamnikarjeva 101, SI-1111 Ljubljana, Slovenia

DOI:

https://doi.org/10.14720//aas.2014.103.2.16

Keywords:

vitis vinifera, grapevines, agrobacterium, hosts, vineyards, damage, genetics, genetic variation, pathogenicity, molecular biology, plasmids, cankers

Abstract

Agrobacterium vitis causes common grape vine (Vitis vinifera L.) crown gall disease that destroyed a lot of Slovenian vineyards more than a decade ago. Eighty isolates of Agrobacterium spp. collected during monitoring in 2006 were identified as A. vitis and A. tumefacies by pehA and multiplex PCR method. Tumor-inducing capacity of these strains was assessed on test plants and with PCR methods for detection of the Ti plasmid responsible for tumor induction. With VCF3/VCR3 primer pair six false negatives and no false positives were detected. The high genetic diversity of pathogenic Agrobacterium spp. strains affects the performance of molecular methods, thus biological test should be performed where results from molecular methods are doubtful.

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Published

4. 12. 2015

Issue

Section

Agronomy section

How to Cite

LAMOVŠEK, J., ZIDARIČ, I., MAVRIČ PLEŠKO, I., UREK, G., & TRDAN, S. (2015). Comparative study of diagnostic methods used for monitoring of common grape vine (Vitis vinifera L.) crown gall (Agrobacterium vitis Ophel & Kerr) in Slovenia. Acta Agriculturae Slovenica, 103(2), 313–321. https://doi.org/10.14720//aas.2014.103.2.16

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