The effect of gamma radiation on seed germination of barley (Hordeum vulgare L.)
DOI:
https://doi.org/10.14720/aas.2014.103.2.15Keywords:
hordeum vulgare, barley, varieties, gamma radiation, germinability, seeds, temperature, greenhouses, induced mutation, geneticsAbstract
The aim of the study was to determine the effect of gamma radiation on barley (Hordeum vulgare L.) seed germination and changes of seed viability associated with aging. The seed samples of the variety 'Astor' were irradiated at the Jožef Štefan Institute, in the Reactor Centre at Podgorica near Ljubljana, in 2006. The samples were irradiated with three different doses: 0.1, 0.2 and 0.4 kGy. After the irradiation, the seeds were stored in a refrigerator at 4 °C. The percentage of seed germination was tested each year after irradiation until 2014, except in the second and the third year. The experiments were based on the Latin square design with 4 replications of each treatment, and were conducted in a greenhouse under controlled temperature at 20 °C. In all years, the samples irradiated with the highest dose (0.4 kGy) exhibited a significantly lower percentage of germination. The germination rate of the samples irradiated with 0.2 and 0.4 kGy decreased significantly with aging, when compared to the control and the samples irradiated with 0.1 kGy. In all years of testing, the percentage of germination of seeds irradiated with 0.1 kGy did not differ from the control, and in the fifth year after irradiation, it was even significantly higher than the control.References
Ahloowalia B.S., Maluszynski M., Nichterlein K. 2004. Global impact of mutation derived varieties. Euphytica 135: 187– 204. DOI: 10.1023/B:EUPH.0000014914.85465.4f DOI: https://doi.org/10.1023/B:EUPH.0000014914.85465.4f
Gladyszewska B. 2011. Estimation of a laser biostimulation dose. Int. Agrophysics 25: 403-405.
Hernandez-Aguilar C., Dominigue s-Pacheco A., Cruz-Orea A., Ivanov R., Carballo-Carballo A., Zepeda-Bautista R., Galindo Soria L. 2009. Laser irradiation effects on field performance of maize seed genotypes. Int. Agrophysics 23: 327-332.
Horn, L., Shimelis, H., 2013. Radio-sensitivity of selected cowpea (Vigna unguiculata) genotypes to varying gamma irradiation doses. Scientific Research and Essays, Vol. 8(40): 1991- 1997.
FAO/IAEA Mutant Varieties Database http://mvgs.iaea.org/Search.aspx (9.9.2014).
Kurowska, M., Labocha-Paw ł owska, A., Gnizda, D., Maluszynski M., Szarejko I. 2012. Molecular analysis of point mutations in a barley genome exposed to MNU and gamma rays. Mutation Research 738–739: 52–70. DOI: 10.1016/j.mrfmmm.2012.08.008 DOI: https://doi.org/10.1016/j.mrfmmm.2012.08.008
Li, Q. H., Wang, S. X., Zhao, Y. M., Xu, J., Gao, T. T., Ren, W. J. 2012. Irradiation dose and effect on germination and growth of desert shrub Nitraria tangutorum Bobr. with two gamma irradiation modes. Pak. J. Bot ., 44(2): 661-666.
Rybiñski, W. 2001. Influence of laser beams combined with chemomutagen (MNU) on the variability of traits and mutation frequency in spring barley. Int. Agrophysics 15: 115-119.
Sacala, E., Demczuk, A., Grzy oe, E., Prooeba-Bialczyk, U., Szajsner, H. 2012. Impact of presowing laser irradiation of seeds on sugar beet properties. Int. Agrophysics 26: 295-300. DOI: 10.2478/v10247-012-0042-6 DOI: https://doi.org/10.2478/v10247-012-0042-6
Stadler L. J. 1928. Mutations in barley induced by X-rays and radium. Science 68: 186–187. DOI: 10.1126/science.68.1756.186 DOI: https://doi.org/10.1126/science.68.1756.186
Wang, H. C., Feng Q. B., Zhang M. A., Yang C. A., Sha W. C., Liu, B. 2010. Alteration of DNA methylation level and pattern in sorghum (Sorghum bicolor L.) pure-lines and inter-line F1 hybrids following low-dose laser irradiation. Journal of Photochemistry a nd Photobiology B: Biology 99: 150–153. DOI: 10.1016/j.jphotobiol.2010.03.011 DOI: https://doi.org/10.1016/j.jphotobiol.2010.03.011
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