Impact of different culture media on hairy roots growth of Valeriana officinalis L.


  • Ali PAKDIN PARIZI Ferdowsi University of Mashhad, Biotechnology Faculty, Agriculture Department, Mashhad, Iran
  • Mohammad FARSI Ferdowsi University of Mashhad, Biotechnology Faculty, Agriculture Department, Mashhad, Iran
  • Ghorban-Ali NEMATZADEH Genetics and agricultural Institute of Tabarestan, Sari, Mazandaran, Iran
  • Amin MIRSHAMSI Ferdowsi University of Mashhad, Biotechnology Faculty, Agriculture Department, Mashhad, Iran



valeriana officinalis, drug plants, growing media, roots, root hairs, nitrogen


Transformed hairy root cultures of Valeriana officinalis were established by infection with Agrobacterium rhizogenes strain ATCC 15834. To determine the effect of different media on the growth of V. officinalis hairy roots, MS, B5 media (1.0X and 0.5X strength), N6 medium and a modified MS medium without phytohormones were used. In addition, different NH4+ to NO3- ratios in MS medium were studied. The effects of these treatments were evaluated after 21 days of culture in relation to hairy root growth. B5 and ½ B5 media were the best basal media for hairy root growth. MS medium supplemented with a 20:20 ratio (mM) of NH4+ to NO3- displayed highest growth rates and biomass yield in hairy root cultures. The present study demonstrated that the composition of culture medium and the ratio of different nitrogen sources have significant impact on the growth of V. officinalis hairy roots.


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4. 12. 2015



Agronomy section

How to Cite

PAKDIN PARIZI, A., FARSI, M., NEMATZADEH, G.-A., & MIRSHAMSI, A. (2015). Impact of different culture media on hairy roots growth of Valeriana officinalis L. Acta Agriculturae Slovenica, 103(2), 299–305.

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