Production of vaccines for treatment of infectious diseases by transgenic plants
DOI:
https://doi.org/10.14720/aas.2016.107.1.19Keywords:
transgenic plants, vaccine production, recombinant proteins, antibodies, infectious diseases, risk assessmentAbstract
Since the first pathogen antigen was expressed in transgenic plants with the aim of producing edible vaccine in early 1990s, transgenic plants have become a well-established expression system for production of alternative vaccines against various human and animal infectious diseases. The main focus of plant expression systems in the last five years has been on improving expression of well-studied antigens such as porcine reproductive and respiratory syndrome (PRRSV), bovine viral diarrhea disease virus (BVDV), footh and mouth disease virus (FMDV), hepatitis B surface antigen (HBsAg), rabies G protein, rotavirus, Newcastle disease virus (NDV), Norwalk virus capsid protein (NVCP), avian influenza virus H5N1, Escherichia coli heat-labile enterotoxin subunit B (LT-B), cholera toxin B (CT-B), human immunodeficiency virus (HIV), artherosclerosis, ebola and anthrax. Significant increases in expression have been obtained using improved expression vectors, different plant species and transformation methods.
References
Aguirreburualde M. S. P., Gomez M. C., Ostachuk A., Wolman F., Albanesi G., Pecora A., Odeon A., Ardila F., Escribano J. M., Dus Santos M. J., Widgorovitz A. 2013. Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants. Veterinary Immunology and Immunopathology, 151: 315- 324, doi: 10.1016/j.vetimm.2012.12.004
Arakawa T., Chong D. K. X., Merritt J. L., Langridge W. H. R. 1997. Expression of cholera toxin B subunit oligomers in transgenic potato plants. Transgenic research, 6: 403-413, doi: 10.1023/A:1018487401810
Arakawa T., Yu J., Langridge W. H. R. 2001. Synthesis of a cholera toxin B subunit-rotavirus NSP4 fusion protein in potato. Plant cell reports, 20: 343-348, doi: 10.1007/s002990000312
Ashraf S., Singh P. K., Yadav D. K., Shahnawaz Md., Mishra S., Sawant S. V., Tuli R. 2005 High level expression of surface glycoprotein of rabies virus in tobacco leaves and its immunoprotective activity in mice. Journal of biotechnology, 119: 1-14, doi: 10.1016/j.jbiotec.2005.06.009
Aziz M. A., Singh S., Kumar A., Bhatnagar R. 2002. Expression of protective antigen in transgenic plants: a step towards edible vaccine against anthrax. Biochemical and biophysical research communications, 299: 345-351, doi: 10.1016/S0006-291X(02)02625-6
Bergeron-Sandoval L.P., Girard A., Ouellet F., Archambault D., Sarhan F. 2011. Production of human rotavirus and Salmonella antigens in plants and elicitation of fljB-specific humoral responses in mice. Molecular biotechnology, 47: 157-168, doi: 10.1007/s12033-010-9324-z
Berinstein A., Vazquez-Rovere C., Asurmendi S., Gomez E., Zanetti F., Zabal O., Tozzini A., Conte Grand D., Taboga O., Caamante G., Barrios H., Hoppa E., Carrillo E. 2005. Mucosal and systemic immunization elicited by Newcastle disease virus (NDV) transgenic plants as antigens. Vaccine, 23: 5583-5589, doi: 10.1016/j.vaccine.2005.06.033
Bertran K., Thomas C., Guo X., Bublot M., Prichard N., Regan J. T., Cox K. M., Gasdaska J. R., Dickey L. F., Kapczynski D. R., Swayne D. E. 2015. Expression of H5 hemagglutinin vaccine antigen in common duckweed (Lemna minor) protects against H5N1 high pathogenicity avian influenza virus challenge in immunized chickens. Vaccine, 33: 3456-3462, doi: 10.1016/j.vaccine.2015.05.076
Bhoo S. H., Lai H., Ma J., Arntzen C. J., Chen Q., Mason H. S. 2011. Expression of an immunogenic ebola immune complex in Nicotiana benthamiana. Plant biotechnology, 9, 7: 807-816, doi: 10.1111/j.1467-7652.2011.00593.x
Carillo C., Widgorovitz A., Oliveros J. C., Zamorano P. I., Sadir A. M., Gomez N., Salinas J., Escribano J. M., Borca M. V. 1998. Protective immune response to foot-and-mouth-disease virus with VP1 expressed in transgenic plants. Journal of Virology, 72, 2: 1688-1690
Carolina C, Francisco A.C. 2004. Tomato transformation and transgenic plant production. Plant Cell Tissue Organ Cultivation, 76: 269-275, doi: 10.1023/B:TICU.0000009249.14051.77
Chan H.T., Chia M.Y., Pang V. F., Jeng C.R., Do Y.Y., Huang P.L. 2013. Oral immunogenicity of porcine reproductive and respiratory syndrome virus antigen expressed in transgenic banana. Plant Biotechnology Journal, 11, 3: 315-324, doi: 10.1111/pbi.12015
Chen Y. R., Wang H. 2002. Transforming HBsAg into peanut and detection of its immunogenicity. Biotechnology Letters, 4: 245-250, doi: 10.1016/j.jviromet.2011.02.001
Chen X., Liu J. 2011. Generation and immunogenicity of transgenic potato expressing the GP5 protein of porcine reproductive and respiratory syndrome virus. Journal of Virological Methods, 173, 1: 153-158
Chia M.Y., Hsiao S.H., Chan H.T., Do Y.Y., Huang P.L., Chang H.W., Tsai Y.C., Lin C.M., Pang V. F., Jeng C.R. 2010. Immunogenicity of recombinant GP5 protein of porcine reproductive and respiratory syndrome virus expressed in tobacco plant. Veterinary Immunology and Immunopathology, 135, 3-4: 234-242, doi: 10.1016/j.vetimm.2011.01.002
Chia M.Y., Hsiao S.H., Chan H.T., Do Y.Y., Huang P.L., Chang H.W., Tsai Y.C, Lin C.M., Pang V. F., Jeng C.R. 2011. Evaluation of the immunogenicity of a transgenic tobacco plant expressing the recombinant fusion protein of GP5 of porcine reproductive and respiratory syndrome virus and B subunit of Eschericia coli heat-labile enterotoxin in pigs. Veterinary Immunology and Immunopathology, 140, 3-4: 215-225
Chikwamba R., McMurray J., Shou H., Frame B., Pegg S. E., Scott P., Mason H. S., Wang K. 2002. Expression of a synthetic E. coli heat-labile enterotoxin B subunit (LT-B) in maize. Molecular breeding, 10: 253-265, doi: 10.1023/A:1020509915672
Choi N. W., Estes M. K., Langridge W. H. R. 2005. Synthesis and assembly of a cholera toxin B subunit-rotavirus VP7 fusion protein in transgenic potato. Molecular biotechnology, 31: 193-202, doi: 10.1385/MB:31:3:193
Chung I. S., Kim C. H., Kim I. K., Hong S. H., Park J. H., Kim J. K. Kim W. Y. 2000. Production of recombinant rotavirus VP6 from a suspension culture of transgenic tomato (Lycopersicon esculentum Mill.) cells. Biotechnology letters, 22: 251-255, doi: 10.1023/A:1005626000329
Chung I. S., Kim C. H., Kim I. K., Hong S. H., Lee Y. H. 2001. Improved production of recombinant rotavirus VP6 in sodium butyrate-supplemented suspension cultures of transgenic tomato (Lycopersicon esculentum Mill.) cells. Biotechnology letters, 23: 1061-1066, doi: 10.1023/A:1010525428239
Circelli P., Donini M., Villani M. E., Benvenuto E., Marusic C. 2010. Efficient Agrobacterium-based transientexpression system for the production of biopharmaceuticals in plants. Bioengineered bugs, 1, 3: 221-224, doi: 10.4161/bbug.1.3.11722
Cueno M. E., Hibi Y., Karamatsu K., Yasutomi Y., Imai K., Laurena A. C., Okamoto T. 2010. Preferential expression and immunogenicity of HIV-1 Tat fusion protein expressed in tomato plant. Transgenic research, 19: 889-895, doi: 10.1007/s11248-009-9358-9
Daniell H., Lee S. B, Panchal T., Wiebe P. O. 2001. Expression of the native cholera toxin subunit B gene and assembly as functional oligomers in transgenic tobacco chloroplasts. Journal of molecular biology, 311, 5: 1001-1009, doi: 10.1006/jmbi.2001.4921
Dong J. L., Liang B. G., Jin Y. S., Zhang W. J., Wang T. 2005. Oral immunization with pBsVP6-transgenic alfalfa protects mice against rotavirus infection. Virology, 339: 153-16, doi: 10.1016/j.virol.2005.06.004
Du G. L., Song C. Z., Zhang G. L., Sun X. G., Liu D. R. 2004. Transgenic Lycium barbarum L. established as HIV capsid protein expression system. Plant molecular biology reporter, 23: 411-416, doi: 10.1007/BF02788889
Dus Santos M. J., Widgorovitz A., Trono K., Rios R. D., Franzone P. M., Gil F., Moreno J., Carillo, Escribano J. M., Borca M. V. 2002. A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plants. Vaccine, 20: 1141-1147, doi: 10.1016/S0264-410X(01)00434-0
Dus Santos M. J., Widgorovitz A. 2005. Transgenic plants for the production of veterinary vaccines. Immunology and Cell Biology, 83: 229- 238, doi: 10.1111/j.1440-1711.2005.01338.x
Dus Santos M. J., Carillo C., Ardila F., Rios R. D., Franzone P., Piccone M. E., Widgorovitz A., Borca M. V. 2005. Development of transgenic alfalfa plants containing the foot and mouth disease virus structural polyprotein gene P1 and its utilization as an experimental immunogen. Vaccine, 23: 1838-1843, doi: 10.1016/j.vaccine.2004.11.014
Firsov A., Tarasenko I., Mitiouchkina T., Ismailova N., Shaloiko L., Vainstein A., Dolgov S. 2015. High-yield expression of M2e peptide of avian influenza virus H5N1 in transgenic duckweed plants. Molecular biotechnology, 57: 653-661, doi: 10.1007/s12033-015-9855-4
Gao Y., Ma Y., Li M., Cheng T., Li SW, Zhang J., Xia NS. 2003. Oral immunization of animals with transgenic cherry tomatillo expressing HBsAg. World Journal of Gastroenterology, 9, 5: 996-1002, doi: 10.3748/wjg.v9.i5.996
Gomez E., Zoth S. C., Asurmendi S., Vazquez-Rovere C., Berinstein A. 2009. Expression of Hemagglutinin-Neuraminidase glycoprotein of Newcastle Disease Virus in agroinfiltrated Nicotiana benthamiana plants. Journal of biotechnology, 144: 337-340, doi: 10.1016/j.jbiotec.2009.09.015
Gomez-Lim M. A. 2014. Newcastle disease vaccines. In: Commercial plant-produced recombinant protein products. Case studies. J. A. Howard & E. E. Hood (Eds.), Springer-Verlag Berlin Heidelberg: 189 https://books.google.si/books?id=ZS9nBAAAQBAJ&pg=PA189&lpg=PA189&dq=newcastle+disease+HN+tobacco+suspension+USDA&source=bl&ots=wM6aAzBxwo&sig=zoOpMo3bjfmGDBfNcZqoGvQlXFI&hl=sl&sa=X&ved=0ahUKEwjzzdqzqarKAhXECCwKHUmrCq0Q6AEIJjAB#v=onepage&q=newcastle %20disease %20HN %20tobacco %20suspension %20USDA&f=false
Gonzales-Rabade N., McGowan E. G., Zhou F., McCabe M. S., Bock R., Dix P. J., Gray J. C., Ma J. K.C. 2011. Immunogenicity of chloroplast-derived HIV-1 p24 and a p24-Nef fusion protein following subcutaneous and oral administration in mice. Plant biotechnology journal, 9: 629-638, doi: 10.1111/j.1467-7652.2011.00609.x
Greco R., Michel M., Guetard D., Cervantes-Gonzales M., Pelucchi N., Wain-Hobson S., Sala F., Sala M. 2008. Production of recombinant HIV-1/HBV virus-like particles in Nicotiana tabacum and Arabidopsis thaliana plants for a bivalent plant-based vaccine. Vaccine, 25, 49: 8228-8240, doi: 10.1016/j.vaccine.2007.09.061
Guan Z. J., Guo B., Huo Y. L., Guan Z. P., Wei Y. H. 2010. Overview of expression of hepatitis B surface antigen in transgenic plants. Vaccine, 28: 7351-7362, doi: 10.1016/j.vaccine.2010.08.100
Guan Z.J., Guo B, Huo Y. L., Guan Z.P., Dai J. K., Wei Y. H. 2013. Recent advances and safety issues of transgenic plant-derived vaccines. Applied microbiology and biotechnology, 97: 2817-2840, 10.1007/s00253-012-4566-2
Guerrero-Andrade O., Loza-Rubio E., Olivera-Flores T., Fehervari-Bone T., Gomez-Lim M. A. 2006. Expression of the Newcastle disease virus fusion protein in transgenic maize and immunological studies. Transgenic research, 15: 455-463, doi: 10.1007/s11248-006-0017-0
Guetard D., Greco R., Cervantes-Gonzales M., Celli S., Kostrzak A., Langlade-Demoyen P., Sala F., Wain-Hobson S., Sala M. 2008. Immunogenicity and tolerance following HIV-1/HBV plant-based oral vaccine administration. Vaccine, 26: 4477-4485, doi: 10.1016/j.vaccine.2008.06.059
Guo Q.Q., Zhang Z.L., Jiang S.J., Ma J.T., Xue W.T., Wu Y.M. 2012. Expression of an avian influenza virus (H5N1) hemagglutinin gene in transgenic Lotus corniculatus. Plant molecular biology reporter, 30: 1117-1124, doi: 10.1007/s11105-012-0423-9
Hahn B.S., Jeon I.S., Jung Y.J., Kim J.B., Park J.S., Ha S.H., Kim K.H., Kim H.M., Yang J.S., Kim Y.H. 2007. Expression of hemagglutinin-neuraminidase protein of Newcastle disease virus in transgenic tobacco. Plant biotechnology reports, 1: 85-92, doi: 10.1007/s11816-007-0012-9
Haq T. A., Mason H. S., Clements J. D., Arntzen C. J. 1995. Oral immunization with a recombinant bacterial antigen produced in transgenic plants. Science, 268, 5211: 714-716, doi: 10.1126/science.7732379
Hayden C. A., Streatfield S. J., Lamphear B. J., Fake G. M., Keener T. K., Walker J. H., Clements J. D., Turner D. D., Tizard I. R., Howard J. A. 2012. Bioencapsulation of the hepatitis B surface antigen and its use as an effective oral immunogen. Vaccine, 30: 2937-2942, doi: 10.1016/j.vaccine.2012.02.072
Hayden C. A., Smith E. M., Turner D. D., Keener T. K., Wong J. C., Walker J. H., Tizard I. R., Jimenez-Flores R., Howard J. A. 2014. Supercritical fluid extraction provides an enhancement to the immune response for orally-delivered hepatitis B surface antigen. Vaccine, 32: 1240-1246, doi: 10.1016/j.vaccine.2014.01.037
Hayden C. A., Fischer M. E., Andrews B. L., Chilton H. C., Turner D. D., Walker J. H., Tizard I. R., Howard J. A. 2015. Oral delivery of wafer made from HBsAg-expressing maize germ induces long-term immunological systemic and mucosal responses. Vaccine, 33: 2881-2886, doi: 10.1016/j.vaccine.2015.04.080
He D.M., Qian K.X., Shen G.F., Li Y.N., Zhang Z.F., Su Z.L., Shao H.B. 2007. Stable expression of foot-and-mouth disease virus protein VP1 fused with cholera toxin B subunit in the potato (Solanum tuberosum). Colloids and Surfaces B: Biointerfaces, 55: 159–163, doi: 10.1016/j.colsurfb.2006.11.043
Hu J., Ni Y., Dryman B. A., Meng X. J., Zhang C. 2012. Immunogenicity study of plant-made oral subunit vaccine against porcine reproductive and respiratory syndrome virus (PRRSV). Vaccine, 30, 12: 2068- 2074, doi: 10.1016/j.vaccine.2012.01.059
Huang Z., Elkin G. Maloney B. J., Beuhner N., Arntzen C. J., Thanavala Y., Mason H. S. 2005. Virus-like particle expression and assembly in plants: hepatitis B and Norwalk viruses. Vaccine, 23: 1851-1858, doi: 10.1016/j.vaccine.2004.11.017
Huang Z., Chen Q., Hjelm B., Arntzen C. J., Mason H. S. 2009. A DNA replicon system for rapid high-level production of virus-like particles in plants. Biotechnology bioengineering, 103, 4: 706-714, doi: 10.1002/bit.22299
Huy N.X., Yang M.S., Kim T.G. 2011. Expression of a cholera toxin B subunit-neutralizing epitope of the porcine epidemic diarrhea virus fusion gene in transgenic lettuce (Lactuca sativa L.). Molecular biotechnology, 48: 201-209, doi: 10.1007/s12033-010-9359-1
ISIS. 2014. Virus vaccine made in tobacco plants to control ebola, report 26/08/14 http://www.isis.org.uk/Virus_Vaccine_Made_In_Tobacco_Plants_to_Control_Ebola.php
Jani D., Meena L. S., Rizwan-ul-Haq Q. M., Singh Y., Sharmal A. K., Tyagi A. K. 2002. Expression of cholera toxin B subunit in transgenic tomato plants. Transgenic research, 11: 447-454, doi: 10.1023/A:1020336332392
Jiang X.L., He Z.M., Peng Z.Q., Qi Y., Chen Q., Yu S.Y. 2007. Cholera toxin B protein in transgenic tomato fruit induces systemic immune response in mice. Transgenic Research, 16: 169-175, doi: 10.1007/s11248-006-9023-5
Kalthoff D., Giritch A., Geisler K., Bettmann U., Klimyuk V., Hehnen H.R., Gleba Y., Beer M. 2010. Immunization with plant-expressed hemagglutinin protects chickens from lethal highly pathogenic avian influenza virus H5N1 challenge infection. Journal of virology, 84, 22: 12002-12010, doi: 10.1128/JVI.00940-10
Kang T.J., Han S.C., Kim M.Y., Kim Y.S., Yang M.S. 2004a Expression of non-toxic mutant of Escherichia coli heat-labile enterotoxin in tobacco chloroplasts. Protein expression and purification, 38: 123-128, doi: 10.1016/j.pep.2004.08.002
Kang T.J., Loc N.H., Yang M.O., Yang M.S. 2004b Modification of the cholera toxin B subunit coding sequence to enhance expression in plants. Molecular breeding, 13: 143-153, doi: 10.1023/B:MOLB.0000018762.27841.7a
Kang T.J., Han S.C., Yang M.S., Jang Y.S. 2006a. Expression of synthetic neutralizing epitope of porcine epidemic diarrhea virus fused with synthetic B subunit of Escherichia coli heat-labile enterotoxin in tobacco plants. Protein expression and purification, 46: 16-22, doi: 10.1016/j.pep.2005.07.026
Kang T.J., Lee W-S., Choi E.G. Kim J.W., Kim B.G., Yang M.S. 2006b. Mass production of somatic embryos expressing Escherichia coli heat-labile enterotoxin B subunit in Siberian ginseng. Journal of biotechnology, 121: 124-133, doi: 10.1385/MB:32:2:093
Kang T.J., Kim B.G., Yang J.Y., Yang M.S. 2006c. Expression of a Synthetic Cholera Toxin B Subunit in Tobacco Using Ubiquitin Promoter and bar Gene as a Selectable Marker. Molecular biotechnology, 32: 93-100, doi: 10.1016/j.jbiotec.2005.07.020
Kapusta J., Modelska A., Figlerowicz M., Pniewski T., Letellier M., Lisowa O., Yusibov V., Koprowski H., Plucienniczak A., Legocki A. B. 1999. A plant-derived edible vaccine against hepatitis B virus. The FASEB Journal, 13: 1796-1799
Karaman S., Cunnick J., Wang K. 2006. Analysis of immune response in young and aged mice vaccinated with corn-derived antigen against Escherichia coli heat-labile enterotoxin. Molecular biotechnology, 32, 1: 31-42, doi: 10.1385/MB:32:1:031
Karaman S., Cunnick J., Wang K. 2012. Expression of the cholera toxin B subunit (CT-B) in maize seeds and a combined mucosal treatment against cholera and traveler’s diarrhea. Plant cell reports, 31: 527-537, doi: 10.1007/s00299-011-1146-3
Kim T. G., Langridge W. H. R. 2003. Assembly of cholera toxin B subunit full-length rotavirus NSP4 fusion protein oligomers in transgenic potato. Plant cell reports, 21: 884-890
Kim T.G., Langridge W. H.R. 2004a. Synthesis of an HIV-1 Tat transduction domain-rotavirus enterotoxin fusion protein in transgenic potato. Plant cell reports, 22: 382-387, doi: 10.1007/s00299-003-0697-3
Kim T.G., Langridge W. H. R. 2004b. Synthesis and assembly of a cholera toxin B subunit SHIV 89.6p Tat fusion protein in transgenic potato. Protein expression and purification, 35: 313-319, doi: 10.1016/j.pep.2004.02.007
Kim T.G., Ruprecht R., Langridge W. H. R. 2004a. Synthesis and assembly of SIVmac gag p27 capsid protein cholera toxin B subunit fusion protein in transgenic potato. Molecular biotechnology, 28: 33-41, doi: 10.1385/MB:28:1:33
Kim T.G., Galloway D. R., Langridge W. H. R. 2004b. Synthesis and assembly of anthrax lethal factor-cholera toxin B-subunit fusion protein in transgenic potato. Molecular biotechnology, 28: 175-183, doi: 10.1385/MB:28:3:175
Kim T.G, Kim M.Y., Kim B.G., Kang T.J., Kim Y.S, Jang Y.S, Arntzen C. J., Yang M.S. 2007. Synthesis and assembly of Escherichia coli heat-labile enterotoxin B subunit in transgenic lettuce (Lactuca sativa). Protein expression and purification, 51: 22-27, doi: 10.1016/j.pep.2006.05.024
Kim Y.S., Kim M.Y., Kim T.G., Yang M.S. 2009. Expression and Assembly of Cholera Toxin B Subunit (CTB) in transgenic carrot (Daucus carota L.). Molecular biotechnology, 41: 8-14, doi: 10.1007/s12033-008-9086-z
Kim T.G, Kim M.Y., Yang M.S. 2010. Cholera toxin B subunit-domain III of dengue virus envelope glycoprotein E fusion protein production in transgenic plants. Protein expression and purification, 74: 236-241, doi: 10.1016/j.pep.2010.07.013
Lai H., He J., Engle M., Diamond M. S., Chen Q. 2012. Robust production of virus-like particles and monoclonal antibodies with geminiviral replicon vectors in lettuce. Plant biotechnology journal, 10, 1: 95-104, doi: 10.1111/j.1467-7652.2011.00649.x
Lai K. S., Yusoff K., Mahmood M. 2013. Functional ectodomain of the hemagglutinin-neuraminidase protein is expressed in transgenic tobacco cells as a candidate vaccine against Newcastle disease virus. Plant cell, tissue and organ culture, 112: 117-121, doi: 10.1007/s11240-012-0214-x
Landry N., Ward B. J., Trepanier S., Montomoli E., Dargis M., Lapini G., Vezina L-P. 2010. Preclinical and clinical development of plant-made virus-like particle vaccine against avian H5N1 influenza. Plos one, 5, 12: 1-9, doi: 10.1371/journal.pone.0015559
Lauterslager T. G. M. Florack D. E. A., Van der Wal T. J., Molthoff J. W., Langeveld J. P. M., Bosch D., Boersma W. J. A., Hilgers L. A. Th. 2001. Oral immunisation of naïve and primed animals with transgenic potato tubers expressing LT-B. Vaccine, 19: 2749-2755, doi: 10.1016/S0264-410X(00)00513-2
Lentz E. M., Mozgovoj M. V., Bellido D., Dus Santos M. J., Wisgorovitz A., Bravo-Almonacid F. F. 2011. VP8* antigen produced in tobacco transplastomic plants confers protection against bovine rotavirus infection in a suckling mouse model. Journal of biotechnology, 156: 100-107, doi: 10.1016/j.jbiotec.2011.08.023
Li D., O’Leary J., Huang Y., Huner N. P. A., Jevnikar A M., Ma S. 2006. Expression of cholera toxin B subunit and the B chain of human insulin as a fusion protein in transgenic tobacco plants. Plant cell reports, 25: 417-424, doi: 10.1007/s00299-005-0069-2
Loc N. H., Bach N. H., Kim T.G., Yang M.S. 2010. Tissue culture and expression of Escherichia coli heat-labile enterotoxin B subunit in transgenic Peperomia pellucida. Protein expression and purification, 72: 82-86, doi: 10.1016/j.pep.2010.02.010
Loc N. H., Bach N. H, Kim T.G., Yang M.S. 2011a. Expression of the Escherichia coli heat-labile enterotoxin B subunit in transgenic watercress (Nasturtium officinale L.). Plant cell tissue and organ culture, 105:39–45, doi. 10.1007/s11240-010-9835-0
Loc N. H., Thinh L. T., Yang M.S., Kim T.G. 2011b. Highly expressed cholera toxin B subunit in the fruit of a transgenic tomato (Lycopersicon esculentum L.). Biotechnology and bioprocess engineering, 16: 576-580
Loc N. H., Long D. T., Kim T.G., Yang M.S. 2014. Expression of Escherichia coli heat-labile enterotoxin B subunit in transgenic tomato (Solanum lycopersicum L.) fruit. Czech journal of genetics and plant breeding, 50, 1: 26-31
Lombardi R., Circelli P., Villani M. E., Buriani G., Nardi L., Coppola V., Bianco L., Benvenuto E., Donini M., Marusic C. 2009. High-level HIV-1 Nef transient expression in Nicotiana benthamiana using the P19 gene silencing suppressor protein of Artichoke
Lou X.M, Zhang Z., Xiong A.S, Wang HK, Peng R.H, Li X. 2005. Hepatitis B surface antigen protein (S1S2) gene expression in transgenic apples. Journal of Fruit Science, 22, 6: 601-610
Loza-Rubio E., Rojas-Anaya E., Lopez J., Olivera-Flores M. T., Gomez-Lim M., Tapia-Perez G. 2012. Induction of a protective immune response to rabies virus in sheep after oral immunization with transgenic maize, expressing the rabies virus glycoprotein. Vaccine, 30: 5551-5556, doi: 10.1016/j.vaccine.2012.06.039
Ma Y., Lin SQ, Gao Y., Zhang J., Lu LX, Xia NS. 2002. Transformation of HBsAg (hepatitis B virus surface antigen) into tomato plants. J Fuijan Agric Forest Univ (Nat Sci Ed), 31: 223-228
Martinez-Gonzales L., Rosales-Mendoza S., Soria-Guerra R. E., Moreno-Fierros L., Lopez-Revilla R., Korban S. S., Guevara-Arauza J. C., Alpuche-Solis A. G. 2011. Oral immunization with a lettuce-derived Escherichia coli heat-labile toxin B subunit induces neutralizing antibodies in mice. Plant cell tissue and organ culture, 107: 441-449, doi: 10.1007/s11240-011-9994-7
Marusic C., Nuttall J., Buriani G., Lico C., Lombardi R., Baschieri S., Benvenuto E., Frigerio L. 2007. Expression, intracellular targeting and purification of HIV Nef variants in tobacco cells. BMC Biotechnology, 7, 12: 1-12
Mason H. S., Lam D. M.K., Arntzen C. J. 1992. Expression of hepatitis B surface antigen in transgenic plants. Proceedings of the National Academy of Sciences of the United States of America, 89: 11745-11749, doi: 10.1073/pnas.89.24.11745
Mason H. S., Ball J. M., Shi J.J., Jiang X., Estes M. K. Arntzen C. J. 1996. Expression of Norwalk virus capsid protein in transgenic tobacco and potato and its oral immunogenicity in mice. Proceedings of the national academy of sciences of the United States of America, 93: 5335-5340, doi: 10.1073/pnas.93.11.5335
Mason H. S., Haq T. A., Clements J. D., Arntzen C. J. 1998. Edible vaccine protects mice against Escherichia coli heat-labile enterotoxin (LT): potatoes expressing a synthetic LT-B gene. Vaccine, 16, 13: 1336-1343, doi: 10.1016/S0264-410X(98)80020-0
Matsumura T., Itchonda N., Tsunemitsu H. 2002. Production of immunogenic VP6 protein of bovine group A rotavirus in transgenic potato plants. Archieves of virology, 147: 1263-1270
McGarvey P. B., Hammond J., Dienelt M. M., Hooper D. C., Fu Z. F., Dietzschold B., Koprowski H., Michaels F. H. 1995. Expression of the rabies virus glycoprotein in transgenic tomatoes. Biotechnology, 13: 1484-1487, doi: 10.1038/nbt1295-1484
Meyers A., Chakuya E., Shephard E., Tanzer F. L., Maclean J., Lynch A., Williamson A.L., Rybicki E. P. 2008. Expression of HIV-1 antigens in plants as potential subunit vaccines. BMC Biotechnology, 8, 53: 1-15, doi: 10.1186/1472-6750-8-53
Mikschofsky H., Konig P., Keil G. M., Hammer M., Schirrmeier H., Broer I. 2009. Cholera toxin B (CTB) is functional as an adjuvant for cytoplasmatic proteins if directed to the endoplasmatic reticulum (ER), but not to the cytoplasm of plants. Plant science, 177: 35-42, doi: 10.1016/j.plantsci.2009.03.010
Mishra S., Yadav D. K., Tuli R. 2006. Ubiquitin fusion enhances cholera toxin B subunit expression in transgenic plants and the plant-expressed protein binds GM1 receptors more efficiently. Journal of biotechnology, 127: 95-108, doi: 10.1016/j.jbiotec.2006.06.002
Modelska A., Dietzschold B., Sleysh N., Fu Z. F., Steplewski K., Hooper D. C., Koprowski H, Yusibov V. 1998. Immunization against rabies with plant-derived antigen. Proceedings of the national academy of science, 95: 2481-2485, doi: 10.1073/pnas.95.5.2481
Moravec T., Schmidt M. A., Herman E. M., Woodford-Thomas T. 2007. Production of Escherichia coli heat labile toxin (LT) B subunit in soybean seed and analysis of its immunogenicity as an oral vaccine. Vaccine, 25: 1647-1657, doi: 10.1016/j.vaccine.2006.11.010
Nelson G., Marconi P., Periolo O., La Torre J., Alvarez M. A. 2012. Immunocompetent truncated E2 glycoprotein of bovine viral diarrhea virus (BVDV) expressed in Nicotiana tabacum plants: A candidate antigen for new generation of veterinary vaccines. Vaccine, 30: 4499- 4504, doi: 10.1016/j.vaccine.2012.04.068
Pan L., Zhang Y., Wang Y., Wang B., Wang W., Fang Y., Jiang S., Lv J., Wang W., Sun Y., Xie Q. 2008. Foliar extracts from transgenic tomato plants expressing the structural polyprotein, P1-2A, and protease, 3C, from foot-and-mouth disease virus elicit a protective response in guinea pigs. Veterinary Immunology and Immunopathology, 121: 83-90, doi: 10.1016/j.vetimm.2007.08.010
Pera F. F. P. G., Mutepfa D. L. R., Khan A. M., Els J. H., Mbewana S., Van Dijk A. A. A., Rybicki E. P., Hitzeroth I. I. 2015. Engineering and expression of a human rotavirus candidate vaccine in Nicotiana benthamiana. Virology journal, 12, 205: 1-11, doi: 10.1186/s12985-015-0436-8
Perea Arango I., Loza-Rubio E., Rojas-Anaya E., Olivera-Flores T., De la Vara L. G., Gomez-Lim M. A. 2008. Expression of the rabies virus nucleoprotein in plants at high-levels and evaluation of immune responses in mice. Plant cell reports, 27: 677-685, doi: 10.1007/s00299-007-0324-9
Perez-Filgueira D. M., Mozgovoj M., Widgorovitz A., Dus Santos M. J., Parreno V., Trono K., Fernandez F. M., Carrillo C., Babiuk L. A., Morris T. J., Borca M. V. 2004a. Passive protection to bovine rotavirus (BRV) infection induced by a BRV VP8* produced in plants using a TMV-based vector. Archives of virology, 149: 2337-2348, doi: 10.1007/s00705-004-0379-7
Perez-Filgueira D. M., Brayfield B. P., Phiri S., Borca M. V., Wood C., Morris T. J. 2004b. Preserved antigenicity of HIV-1 p24 produced and purified in high yields from plants inoculated with a tobacco mosaic virus (TMV)-derived vector. Journal of virological methods, 121: 201-208, doi: 10.1016/j.jviromet.2004.06.022
Piron R., De Koker S., De Paepe A., Goossens J., Grooten J., Nauwynck H., Depicker A. 2014. Boosting In Planta Production of Antigens Derived from the Porcine Reproductive in Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity. PLOS ONE, 9, 3: 1-16, doi: 10.1371/journal.pone.0091386
Pniewski T., Kapusta J., Bociag P., Wojciechowicz J., Kostrzak A., Gdula M., Fedorowicz-Stronska O., Wojcik P., Otta H., Samardakiewicz S., Wolko B., PŁucienniczak A. 2011. Low-dose oral immunization with lyophilized tissue of herbicide-resistant lettuce expressing hepatitis B surface antigen for prototype plant-derived vaccine tablet formulation. Journal of applied genetics, 107: 441 – 449, doi: 10.1007/s13353-010-0001-5
Rattanapisit K., Bhoo S. H., Hahn T. R., Mason H. S., Phoolcharoen W. 2013. Rapid transient expression of cholera toxin B subunit (CTB) in Nicotiana benthamiana. In vitro cellular and developmental biology- plant, 49: 107-113, doi: 10.1007/s11627-012-9484-6
Ravin N. V., Kotlyarov R. Y., Mardanova E. S., Kuprianov V. V., Migunov A. I., Stepanova L. A., Tsybalova L. M., Kiselev O. I., Skryabin K. G. 2012. Plant-produced recombinant influenza vaccine based on virus-like HBc particles carrying an extracellular domain of M2 protein. Biokhimiya, 77, 1: 43-52, doi: 10.1134/s000629791201004x
Renukaradhya G. J., Meng X-J., Calvert J. G., Roof M., Lager K. M. 2015. Inactivated and subunit vaccines against porcine reproductive and respiratory syndrome: current status and future direction. Vaccine, 33, 27: 3065- 3072, doi: 10.1016/j.vaccine.2015.04.102
Rojas-Anaya E., Loza-Rubio E., Olivera-Flores M. T., Gomez-Lim M. 2009. Expression of rabies virus G protein in carrots (Daucus carota). Transgenic research, 18: 911-919, doi: 10.1007/s11248-009-9278-8
Rosales-Mendoza S., Soria-Guerra R. E., Olivera-Flores M. T., Lopez-Revilla R., Arguello-Astroga G. R., Jimenez-Bremont J. F., Garcia-de la Cruz R. F., Loyola-Rodriguez J. P., Alpuche-Solis A. G. 2007. Expression of Escherichia coli heat-labile enterotoxin b subunit (LTB) in carrot (Daucus carota L.). Plant Cell Reports, 26: 969–976, doi: 10.1007/s00299-007-0310-2
Rosales-Mendoza S., Soria-Guerra R. E., Lopez-Revilla R., Moreno-Fierros L., Alpuche-Solis A. G. 2008. Ingestion of transgenic carrots expressing the Escherichia coli heat-labile enterotoxin B subunit protects mice against cholera toxin challenge. Plant Cell Reports, 27: 79–84, doi: 10.1007/s00299-007-0439-z
Rosales-Mendoza S. 2015. Current developments and future prospects for plant-made biopharmaceuticals against rabies. Molecular biotechnology, 57: 869-879, doi: 10.1007/s12033-015-9880-3
Roy S., Tyagi A., Tiwari S., Singh A., Sawant S. V., Singh P. K., Tuli R. 2010. Rabies glycoprotein fused with B subunit of cholera toxin expressed in tobacco plants folds into biologically active pentameric protein. Protein expression and purification, 70: 184-190, doi: 10.1016/j.pep.2009.10.002
Rukavtsova E. B., Rudenko N. V., Puchko E. N., Zakharchenko N. S., Buryanov Y. I. 2015. Study of the immunogenicity of hepatitis B surface antigen synthesized in transgenic potato plants with increased biosafety. Journal of Biotechnology, 203: 84-88, doi: 10.1016/j.jbiotec.2015.03.019
Salazar-Gonzales J. A., Rosales-Mendoza S. 2013. A perspective for artherosclerosis vaccination: is there a place for plant-based vaccines? Vaccine, 31: 1364-1369, doi: 10.1016/j.vaccine.2013.01.005
Salazar-Gonzales J. A., Rosales-Mendoza S., Romero-Maldonado A., Monreal-Escalante E., Uresti-Rivera E. E., Banuelos-Hernandez B. 2014. Production of a plant-derived immunogenic protein targeting ApoB100 and CETP: toward a plant-based artherosclerosis vaccine. Molecular biotechnology, 56: 1133-1142, doi: 10.1007/s12033-014-9793-6
Santi L., Batchelor L., Huang Z., Hjelm B., Kilbourne J., Arntzen C. J., Chen Q., Mason H. S. 2008. An efficient plant viral expression system generating orally immunogenic Norwalk viral-like particles. Vaccine, 26: 1846-1854, doi: 10.1016/j.vaccine.2008.01.053
Scotti N., Alagna F., Ferraiolo E., Formisano G., Sannino L., Buonaguro L., De Stradis A., Vitale A., Monti L., Grillo S., Buonaguro F. M., Cardi T. 2009. High-level expression of the HIV-1 Pr55gag polyprotein in transgenic tobacco chloroplasts. Planta, 229: 1109-1122, doi: 10.1007/s00425-009-0898-2
Sharma M. K., Jangi D., Thungapathra M., Gautam J. K., Meena L. S., Singh Y., Ghosh A., Tyagi A. K., Sharma A. K. 2008. Expression of accessory colonization factor subunit A (ACFA) of Vibrio cholerae and ACFA fused to cholera toxin B subunit in transgenic tomato (Solanum lycopersicum). Journal of biotechnology, 135: 22-27, doi: 10.1016/j.jbiotec.2008.03.002
Shekhawat U. K. S., Ganapathi T. R., Srinivas L. 2010. Expression of hepatitis B small surface antigen in Santalum album embryogenic cell suspension cultures. Biologia plantarum, 54, 4: 720-724, doi: 10.1007/s10535-010-0128-6
Shoji Y., Farrance C. E., Bi H., Shaloul M., Green B., Manceva S., Rhee A., Ugulava N., Roy G. Musivchuk K., Chichester J. A., Mett V., Yusibov V. 2009. Immunogenicity of hemagglutinin from A/Bar-headed Goose/Qinghai/1A/05 and A/Anhui/1/05 strains of H5N1 influenza viruses produced in Nicotiana benthamiana plants. Vaccine, 27: 3467-3470, doi: 10.1016/j.vaccine.2009.01.051
Shulga N. Y., Rukavtsova, E. B. Krymsky M. A., Borisova V. N., Melnikov V. A., Byrkov V. A. 2004. Expression and characterization of hepatitis B surface antigen in transgenic potato plants. Biochemistry (Mosc), 69:1158–1164, doi: 10.1023/B:BIRY.0000046891.46282.c8
Singh A., Srivastava S., Chouskey A., Panwar B. S., Verma P. C., Roy S. Singh P. K., Saxena G. Tuli R. 2015. Expression of rabies glycoprotein and ricin toxin B chain (RGP–RTB) fusion protein in tomato hairy roots: a step towards oral vaccination for rabies. Molecular biotechnology, 57: 359-370, doi: 10.1007/s12033-014-9829-y
Smith M. L., Mason H. S., Shuler M. L. 2002. Hepatitis surface (HBsAg) expression in plant cell culture: kinetics of antigen accumulation in batch culture and its intracellular form. Biotechnology and Bioengineering, 80: 812-822, doi: 10.1002/bit.10444
Soh H. S., Chung H. Y., Lee H. H., Ajjappala H., Jang K., Par J.H., Sim J.S., Lee G. Y., Lee H. J., Han Y. H., Lim J. W., Choi I., Chung I. S., Hahn B.S. 2015. Expression and functional validation of heat-labile enterotoxin B (LTB) and cholera toxin B (CTB) subunits in transgenic rice (Oryza sativa). Springerplus, 4, 148: 1-14, doi: 10.1186/s40064-015-0847-4
Souza A. C., Vasques R. M., Ingone-Nagata A. K., Lacorte C., Madaner F. R., Ferreira Noronha E., Nagata T. 2013. Expression and assembly of Norwalk virus-like particles in plants using a viral RNA silencing suppressor gene. Applied microbiology and biotechnology, 97: 9021-9027, doi: 10.1007/s00253-013-5077-5
Srinivas L., Sunil-Kumar G. B., Ganapathi T. R., Revathi C. J., Bapat V. A. 2008. Transient and stable expression of hepatitis B surface antigen in tomato (Lyopersicon esculentum L.). Plant biotechnology reports, 2: 1-6, doi: 10.1007/s11816-008-0041-z
Starodubova E. S., Preobrazhenskaia O. V., Kuzmenko Y. V., Latanova A. A., Yarygina E. I., Karpov V. L. 2015. Rabies Vaccines: Current Status and Prospects for Development. Molecular biotechnology, 49, 4: 513-519, doi: 10.1134/s0026893315040172
Streatfield S. J., Jilka J. M., Hood E. E., Turner D. D., Bailey M. R., Mayor J. M., Woodard S. L., Beifuss K. K., Horn M. E., Delaney D. E., Tizard I. R., Howard J. A. 2001. Plant-based vaccines: unique advantages. Vaccine, 19: 2742-2748, doi: 10.1016/S0264-410X(00)00512-0
Sunil-Kumar G. B., Ganapathi T. R., Revathi C. J., Srinivas L., Bapat V. A. 2005. Expression of hepatitis B surface antigen in transgenic banana plants. Planta, 222: 484-493, doi: 10.1007/s00425-005-1556-y
Sunil-Kumar G. B., Ganapathi T. R., Revathi C. J., Srinivas L., Bapat V. A. 2007. Hepatitis B surface antigen expression in NT-1 cells of tobacco using different expression cassettes. Biologia Plantarium, 51, 3: 467-471, doi: 10.1007/s10535-007-0098-5
Tacket C. O., Mason H. S., Losonsky G., Clements J. D., Levine M. M., Arntzen C. J. 1998. Immunogenicity in humans of a recombinant bacterial antigen delivered in transgenic potato. Nature, 4, 5: 607 – 609, doi: 10.1038/nm0598-607
Tacket C. O., Mason H. S., Losonsky G. Estes M. K., Levine M. M., Arntzen C. J. 2000. Human immune responses to a novel Norwalk virus vaccine delivered in transgenic potatoes. The journal of infectious diseases, 182: 302-305, doi: 10.1086/315653
Tacket C. O., Pasetti M. F., Edelman R., Howard J. A., Streatfield S. 2004. Immunogenicity of recombinant LT-B delivered orally to humans in transgenic corn. Vaccine, 22: 4385-4389, doi: 10.1016/j.vaccine.2004.01.073
Tang W., Page M. 2013. Inducible expression of Norwalk virus capsid protein gene in plant cell suspension cultures. In vitro cellular and developmental biology- plant, 49: 129-136, doi: 10.1007/s11627-012-9487-3
Tarasenko I. V., Taranov A. I., Firsov A. P., Dolgov S. V. 2013. Expression of the nucleotide sequence for the M2e peptide of avian influenza virus in transgenic tobacco plants. Applied biochemistry and microbiology, 49, 8: 695-701, doi: 10.1134/S0003683813080061
Thanavala Y., Mahoney M., Pal S., Scott A., Richter L., Natarajan N., Goodwin P., Arntzen C. J., Mason H. S. 2005. Immunogenicity in humans of an edible vaccine for hepatitis B. PNAS, 102, 9: 3378-3382, doi: 10.1073/pnas.0409899102
Tiwari S., Mishra D. K., Roy S., Singh A., Singh P. K., Tuli R. 2009. High level expression of a functionally active cholera toxin B: rabies glycoprotein fusion protein in tobacco seeds. Plant cell reports, 28: 1827-1836, doi: 10.1007/s00299-009-0782-3
Unni S. C., Soniya E. V. 2010. Transgenic Cucumis sativus expressing the hepatitis B surface antigen. Plant molecular biology reporter, 28: 627-634, doi: 10.1007/s11105-010-0179-z
Uribe-Campero L., Monroy-García A., Durán-Meza A. L., Villagrana-Escareño M. V., Ruíz-García J., Hernández J., Núñez-Palenius H. G., Gómez-Lim M.A. 2015. Plant-based porcine reproductive and respiratory syndrome virus VLPs induce an immune response in mice. Research in Veterinary Science, 102: 59- 66, doi: 10.1016/j.rvsc.2015.07.012
USDA issues license for plant cell produced Newcastle disease vaccine for chickens. 2006 http://www.thepoultrysite.com/poultrynews/8949/usda-issues-license-for-plant-cell-produced-newcastle-disease-vaccine-for-chickens/
Wagner B., Hufnagl K., Radauer C., Wagner S., Baier K., Scheiner O., Wiedermann U., Breiteneder H. 2004. Expression of the B subunit of the heat-labile enterotoxin of Escherichia coli in tobacco mosaic virus-infected Nicotiana benthamiana plants and its characterization as mucosal immunogen and adjuvant. Journal of immunological methods, 287: 203-215, doi: 10.1016/j.jim.2004.02.001
Wang X.G., Zhang G.H., Liu C.X., Zhang Y.H., Xiao C.Z., Fang R.X. 2001. Purified cholera toxin B subunit from transgenic tobacco plants possesses authentic antigenicity. Biotechnology and bioengineering, 72: 490-494, doi: 10.1002/1097-0290(20010220)72:4<490::AID-BIT1011>3.0.CO;2-0
Wang YQ, Li T. 2008. Transformation of HBsAg into tomato and production of transgenic tomato plants. J Southwest Univ (Nat Sci Ed), 30: 78-83
Ward B. J., Landry N., Trepanier S., Mercier G., Dargis M., Couture M., D’Aoust M. A., Vezina L. P. 2014. Human antibody response to N-glycans present on plant-made influenza virus-like particle (VLP) vaccines. Vaccine, 32: 6098-6106, doi: 10.1016/j.vaccine.2014.08.079
Widgorovitz A., Carillo C., Dus Santos M. J., Trono K., Peralta A., Gomez M. C., Rios R. D., Franzone P. M., Sadir A. M., Escribano J. M., Borca M. V. 1999. Induction of a protective antibody response to foot and mouth disease virus in mice following oral or parenteral immunization with alfalfa transgenic plants expressing the viral structural protein VP1. Virology, 255: 347-353, doi: 10.1006/viro.1998.9590
Wu Y.Z., Li J.T., Mou Z.R., Fei L., Ni B., Geng M., Jia Z.C., Zhou W., Zou L.Y., Tang Y. 2003. Oral immunization with rotavirus VP7 expressed in transgenic potatoes induced high titers of mucosal neutralizing IgA. Virology, 31: 337- 342, doi: 10.1016/S0042-6822(03)00280-0
Yang Y.M., Lia X., Yang H., Qian Y., Zhang Y., Fang R.X., Chen X.Y. 2011. Immunogenicity and virus-like particle formation of rotavirus capsid proteins produced in transgenic plants. Science china life sciences, 54, 1: 82-89, doi: 10.1007/s11427-010-4104-3
Yang Z.Q., Liu Q.Q., Pan Z.M., Yu H.X. Jiao X.A. 2007. Expression of the fusion glycoprotein of Newcastle disease virus in transgenic rice and its immunogenicity in mice. Vaccine, 25: 591-598, doi: 10.1016/j.vaccine.2006.08.016
Yu J., Langridge W. H. R. 2001. A plant-based multicomponent vaccine protects mice from enteric diseases. Nature biotechnology, 19: 548–552, doi: 10.1038/89297
Yu J., Langridge W. 2003. Expression of rotavirus capsid protein VP6 in transgenic potato and its oral immunogenicity in mice. Transgenic research, 12: 163-169, doi: 10.1023/A:1022912130286
Yusibov V., Modelska A., Steplewski K., Agadjanyan M., Weiner D., Hooper D. C., Koprowski H. 1997. Antigens produced in plants by infection with chimeric plant viruses immunize against rabies virus and HIV-1. Proceedings of the national academy of science, 94: 5784-5788, doi: 10.1073/pnas.94.11.5784
Yusibov V., Hooper D. C., Spitsin S. V., Fleysh N., Kean R. B., Mikheva T., Deka D., Karasev A., Cox S. Randall J., Koprowski H. 2002. Expression in plants and immunogenicity of plant virus-based experimental rabies vaccine. Vaccine, 20: 3155-3164, doi: 10.1016/S0264-410X(02)00260-8
Zhang G. G., Rodrigues L., Rovinski B., White K. A. 2002. Production of HIV-1 p24 Protein in Transgenic Tobacco Plants. Molecular biotechnology, 20: 131-136, doi: 10.1385/MB:20:2:131
Zhang S.Z., Zhang G.L., Rong T.Z., Pan L., Zhou P., Zhang Y.G. 2011. Transformation of two VP1 genes of O- and Asia 1-type foot-and-mouth disease virus into maize. Agricultural Sciences in China, 10, 5: 661-667, doi: 10.1016/S1671-2927(11)60048-5
Zhao X. Y., Zhang X. D., Zhang T. 2002. Cloning and expression of hepatitis B virus surface antigen in carrots. Progress in Microbiology and Immunology, 30, 1:1
Zhou B., Zhang Y., Wang X. Dong J., Wang B., Han C., Yu J., Li D. 2010. Oral administration of plant-based rotavirus VP6 induces antigen-specific IgAs, IgGs and passive protection in mice. Vaccine, 28: 6021-6027, doi: 10.1016/j.vaccine.2010.06.094
Downloads
Published
Issue
Section
License
Copyright (c) 2016 Kristina Ledl, Zlata Luthar
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.