Effect of foliar-applied silicon on photochemistry, antioxidant capacity and growth in maize plants subjected to chilling stress


  • Ghader HABIBI Department of Biology, Payame Noor University (PNU), Iran




chilling stress, lipid peroxidation, non-photochemical quenching, silicon, Zea mays


Low temperature is one of the major adverse climatic factors that suppress plant growth and sustainable agricultural development. In these climate conditions, silicon (Si) can mitigate various abiotic stresses including low temperature. In this study, the roles of foliar-applied silicon (10 mM potassium metasilicate) in enhancing tolerance to chilling stress were investigated in maize (Zea mays ‘Fajr’) plants. The low temperature stress caused significant reduction of plant growth and relative water content; however, Si ameliorated these effects. Si supply in maize exhibited a significantly positive effect on accumulation of free amino acids, and reduced the necrotic leaf area. The decrease in maximum quantum yield of PSII (Fv/Fm) was reversible during recovery, but not in the non-Si-treated leaves. This can be explained by enhancement of protective pigments; carotenoid and anthocyanin leading to the protection of PSII from damage. Additionally, analysis of OJIP transients revealed that Si reduced cold damaging effect on performance index (PIabs) and Fv/Fm through improvement of excitation energy trapping (TR0/CS) and electron transport (ET0/CS) per excited cross-section of leaf. The malondialdehyde (MDA) concentration, which was significantly increased under chilling stress, was decreased by Si. The reduced glutathione and ascorbate concentrations were higher in Si-treated plants as compared to those without application of Si under chilling stress. These results indicated that Si could enhance the chilling stress tolerance of maize plants through improving the biomass accumulation, maintaining a high level of glutathione, ascorbic acid, protein, protective pigments, and enhancing the photochemical reactions. This study also suggests that the foliar-applied Si increases recovery ability from chilling injury.


Battal, P., Erez, M.E., Turker, M., Berber, I. 2008. Molecular and physiological changes in maize (Zea mays) induced by exogenous NAA, ABA and MeJa during cold stress. Annales Botanici Fennici. 45: 173–185. DOI: 10.5735/085.045.0302

Bradford, M.M. 1967. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248–254. DOI: 10.1016/0003-2697(76)90527-3

Broadley, M., Brown, P., Cakmak, I., Ma, J.F., Rengel, Z. and Zhao, F.P. 2011. Beneficial Elements. In: "Marschner's Mineral Nutrition of Higher Plants" (Ed.): Marschner, P.. UK, Academic Press, London. PP. 249–269.

Baker, N.R. and Rosenqvist, E. 2004. Applications of chlorophyll fluorescence can improve crop production strategies: an examination of future possibilities. J. Exp. Bot., 55: 1607–1621. DOI: 10.1093/jxb/erh196

Balouchi, H.R. 2010. Screening wheat parents of mapping population for heat and drought tolerance, detection of wheat genetic variation. Int. J. Biol. Life Sci., 6: 56–66.

Dallagnol, L.J., Rodrigues, F.A., Tanaka, F.A.O., Amorim, L. and Camargo, L.E.A. 2012. Effect of potassium silicate on epidemic components of powdery mildew on melon. Plant Pathol., 61: 323–330. DOI: 10.1111/j.1365-3059.2011.02518.x

Fu, J., Sun, Y., Chu, X., Xu, Y. and Hu, T. 2014. Exogenous 5-aminolevulenic acid promotes seed germination in Elymus nutans against oxidative damage induced by cold stress. PLoS One 9: e107152. DOI: 10.1371/journal.pone.0107152

Guntzer, F., Keller, C. and Meunier, J.D. 2011. Benefits of plant Si for crops: a review. Agron. Sustain. Dev., 32: 201–213. DOI: 10.1007/s13593-011-0039-8

Habibi, G. and Hajiboland, R. 2013. Alleviation of drought stress by Si supplementation in maize (Pistacia vera L.) plants. Folia Hort., 25: 21–29.

Habibi, G. and Hajiboland, R. 2012. Comparison of photosynthesis and antioxidative protection in Sedum album and Sedum stoloniferum (Crassulaceae) under water stress. Photosynthetica, 50: 508–518. DOI: 10.1007/s11099-012-0066-y

Habibi, G. 2014a. Role of Trace Elements in Alleviating Environmental Sress. In: "Emerging Technologies and Management of Crop Stress Tolerance Biological Techniques" (Eds.): Ahmad, P. and Rasool, S. Elsevier, Boston, USA, PP. 313–331.

Habibi, G. 2014b. Silicon supplementation improves drought tolerance in canola plants. Russian J. Plant Physiol., 61: 784–791. DOI: 10.1134/S1021443714060077

Huang, H.Y., Zhang, Q., Zhao, L.P., Feng, J.N. and Peng, C.L. 2010. Does lutein play a key role in the protection of photosynthetic apparatus in Arabidopsis under severe oxidative stress? Pak. J. Bot., 42: 2765–2774.

Huber, S.C., Huber, J.L., Campbell, W.H. and Redinbaugh, M.G. 1992. Apparent dependence of the light activation of nitrate reductase and sucrose phosphate synthase activities in spinach leaves on protein synthesis. Plant Cell Physiol., 33: 639–646.

Hwang, M. and Ederer, G.M. 1975. Rapid hippurate hydrolysis method for presumptive identification of group B streptococci. J. Clin. Microbiol., 1: 114–115.

Irigoyen, J.J., Juan, J.P.D. and Diaz, M.S. 1996. Drought enhances freezing tolerance in a freezing-sensitive maize (Zea mays). New Phytol., 134: 53–59. DOI: 10.1111/j.1469-8137.1996.tb01145.x

Ivanov, A.G., Sane, P.V., Krol, M., Gray, G.R., Balseris, A., Savitch, L.V., Oquist, G. and Hüner, N.P.A. 2006. Acclimation to temperature and irradiance modulates PSII charge recombination. FEBS Lett, 580: 2797-2802. DOI: 10.1016/j.febslet.2006.04.018

Jaiswal, P.C. 2004. Soil, Plant and Water Analysis, (Ed.): Kalyani Publishers, New Delhi.

Krall, J.P. and Edwards, G.E. 1992. Relationship between photosystem II activity and CO2 fixation in leaves. Physiol. Plant., 86: 180–187. DOI: 10.1111/j.1399-3054.1992.tb01328.x

Jiao-jing, L., Shao-hang, L., Pei-lei, X., Xiu-juan, W. and Ji-gang, B. 2009. Effects of exogenous Si on the activities of antioxidant enzymes and lipid peroxidation in freezing-stressed cucumber leaves. Agric. Sci. China, 8: 1075–1086. DOI: 10.1016/S1671-2927(08)60315-6

Krasensky, J. and Jonak, C. 2012. Drought, salt and temperature stress-induced metabolic rearrangements and regulatory networks. J. Exp. Bot., 63: 1593–1608. DOI: 10.1093/jxb/err460

Liang, Y., Zhuc, J., Li, Z., Chua, G., Dingc, Y., Zhangc, J. and Sun, W. 2008. Role of Si in enhancing resistance to freezing stress in two contrasting winter wheat cultivars. Environ. Exp. Bot., 64: 286–294. DOI: 10.1016/j.envexpbot.2008.06.005

Lichtenthaler, H.K. and Wellburn, A.R. 1985. Determination of total carotenoids and chlorophylls a and b of leaf in dfferent solvents. Biochem. Soc. Trans., 11: 591–592. DOI: 10.1042/bst0110591

Liu, J., Lin, S., Xu, P., Wang, X. and Bai, J. 2009. Effects of exogenous silicon on the activities of antioxidant enzymes and lipid peroxidation in chilling-stressed cucumber leaves. Agric. Sci. China, 8: 1075–1086. DOI: 10.1016/S1671-2927(08)60315-6

Liu, P., Yin, L., Deng, X., Wang, S., Tanaka, K. and Zhang, S. 2014. Aquaporin-mediated increase in root hydraulic conductance is involved in Si-induced improved root water uptake under osmotic stress in Sorghum bicolor L. J. Exp. Bot., 65: 4747–4756. DOI: 10.1093/jxb/eru220

Magné, C., Saladin, G., Clément, C. 2006. Transient effect of the herbicide flazasulfuron on carbohydrate physiology in Vitis vinifera. Chemosphere, 62: 650–657. DOI: 10.1016/j.chemosphere.2005.04.119

Marczak, L., Kachlicki, P., Kozniewski, P., Skirycz, A., Krajewski, P. and Stobiecki, M. 2008. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry monitoring of anthocyanins in extracts from Arabidopsis thaliana leaves. Rapid Commun. Mass. Sp., 22: 3949–3956. DOI: 10.1002/rcm.3819

Maxwell, K. and Johnson, G.N. 2000. Chlorophyll fluorescence – a practical guide. J. Exp. Bot., 51: 659–668. DOI: 10.1093/jexbot/51.345.659

Oxborough, K. 2004. Using Chlorophyll a Fluorescence Imaging to Monitor Photosynthetic Performance. In: "Chlorophyll a Fluorescence, A Signature of Photosynthesis" (Ed.): Papageorgiou, G.C. Springer, Dordrecht, PP. 409–428. DOI: 10.1007/978-1-4020-3218-9_15

Saqib, M., Zörb, C. and Schubert, S. 2008. Si-mediated improvement in the salt resistance of wheat (Triticum aestivum) results from increased sodium exclusion and resistance to oxidative stress. Func. Plant Biol., 35: 633–639.

Singh, N., Ma, L.Q., Srivastava, M. and Rathinasabapathi, B. 2006. Metabolic adaptations to arsenic-induced oxidative stress in Pteris vittata L and Pteris ensiformis L. Plant Sci., 170: 274–282. DOI: 10.1016/j.plantsci.2005.08.013

Sonobe, K., Hattori, T., An, P., Tsuji, W., Eneji, A.E., Kobayashi, S., Kawamura, Y., Tanaka, K. and Inanaga, S. 2011. Effect of Si application on sorghum root responses to water stress. J. Plant Nutr., 34: 71–82. DOI: 10.1080/01904167.2011.531360

Strasser, B.J., Strasser, R.J. 1995. Measuring fast fluorescence transients to address environmental questions: The JIP-test. In: Mathis, P. (Ed.), Photosynthesis: From Light to Biosphere, vol. V. Kluwer Academic Publishers, The Netherlands, pp. 977-980. DOI: 10.1007/978-94-009-0173-5_1142

Strasser, R.J., Srivastava, A., Tsimilli-Michael, M. 2000. The fluorescent transient as a tool to characterise and screen photosynthetic samples. In: Yunus, M., Pathre, U., Mohanty, P. (Eds.), Probing Photosynthesis: Mechanisms, Regulation and Adaptation. Taylor and Francis, London, pp. 445-483.

Strasser, R.J., Tsimilli-Michael, M., Srivastava, A. 2004. Analysis of the chlorophyll a fluorescence transient. In: Papageorgiou, G.C., Govindjee (Eds.), Chlorophyll a Fluorescence: A Signature of Photosynthesis. Springer, Dordrecht, pp. 321-362. DOI: 10.1007/978-1-4020-3218-9_12

Suzuki, N., Koussevitzky, S., Mittler, R. and Miller, G. 2012. ROS and redox signalling in the response of plants to abiotic stress. Plant Cell Environ., 35: 259–270. DOI: 10.1111/j.1365-3040.2011.02336.x

Wagner, G.J. 1979. Content and vacuole/extra vacuole distribution of neutral sugars free amino acids, and anthocyanins in protoplast. Plant Physiol., 64: 88–93. DOI: 10.1104/pp.64.1.88

Waśkiewicz, A., Beszterda, M. and Goliński, P. 2014. Nonenzymatic Antioxidants in Plants. In: "Antioxidant Networks and Signaling Oxidative Damage to Plants" (Eds.): Ahmad, P. Elsevier, USA, PP. 201–234. DOI: 10.1016/b978-0-12-799963-0.00007-1

Yin, L., Wang, S., Li, J., Tanaka, K. and Oka, M. 2013. Application of Si improves salt tolerance through ameliorating osmotic and ionic stresses in the seedling of Sorghum bicolor. Acta Physiol. Plant., 35: 3099–3107. DOI: 10.1007/s11738-013-1343-5

Zhang, Q., Zhang, J.Z., Chow, W.S., Sun, L.L., Chen, J.W., Chen, Y.J. and Peng, C.L. 2011. The influence of low temperature on photosynthesis and antioxidant enzymes in sensitive banana and tolerant plantain (Musa sp.) cultivars. Photosynthetica, 49: 201–208. DOI: 10.1007/s11099-011-0012-4



6. 04. 2016



Agronomy section

How to Cite

HABIBI, G. (2016). Effect of foliar-applied silicon on photochemistry, antioxidant capacity and growth in maize plants subjected to chilling stress. Acta Agriculturae Slovenica, 107(1), 33–43. https://doi.org/10.14720/aas.2016.107.1.04

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