The impact of salicylic acid on some physiological responses of Artemisia aucheri Boiss. under in vitro drought stress


  • Jalil ABBASPOUR Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran
  • Aliakbar EHSANPOUR Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran



Artemisia aucheri Boiss., salicylic acid, drought stress, polyethylene glycol, growth, phenylalanine ammonia-lyase, tyrosine ammonia-lyase


Salicylic acid (SA) is an important plant regulator which is involved in growth, development, and response to stress. This study was aimed to evaluate some physiological and biochemical responses of Artemisia aucheri Boiss. under drought stress after exogenous SA treatment. Experiment was performed in vitro. Polyethylene glycol (PEG/6000) with 0, 2 and 4 % (w/v) was used in MS medium to simulate drought stress and different concentrations of SA (0, 0.01 and 0.1mM) were added. After four weeks, SA alleviated the negative effects of PEG on dry and fresh mass as well as chlorophyll and carotenoid contents. Under drought stress, application of SA decreased storage polysaccharides and increased soluble carbohydrates respectively. Although PEG had no significant effect on flavonoid content, it increased significantly anthocyanin and total phenol content, total antioxidant capacity, PAL (phenylalanine ammonia-lyase) and TAL (tyrosine ammonia-lyase) activity and SA treatment improved these parameters significantly. According to the current data, it was concluded that SA increased drought tolerance of Artemisia aucheri by increasing biosynthesis of phenolic compounds, improvement of TAL and PAL activity as well as also by increased content of soluble carbohydrates.


Ahmed I.M., Nadira U.K, Bibi N., Cao F., He X., Zhang G., Wu F. 2015. Secondary metabolism and antioxidants are involved in the tolerance to drought and salinity, separately and combined, in Tibetan wild barley. Environmental and Experimental Botany, 111:1-12. Doi: 10.1016/j.envexpbot.2014.10.003

Asada K. 1999. The water–water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons. Annual Review of Plant Biology, 150: 601-639. Doi: 10.1146/annurev.arplant.50.1.601

Asghari G., Jalali M., Sadoughi E. 2012. Antimicrobial activity and chemical composition of essential oil from the seeds of Artemisia aucheri Boiss. Jundishapur Journal of Natural Pharmaceutical Products, 7: 11–15. Doi: 10.17795/jjnpp-3530

Askari E., Ehsanzadeh P. 2015. Drought stress mitigation by foliar application of salicylic acid and their interactive effects on physiological characteristics of fennel (Foeniculum vulgare Mill.) genotypes. Acta Physiologiae Plantarum, 37:2-14. Doi: 10.1007/s11738-014-1762-y

Bandurska H. 2000. Does proline accumulated in leaves of water deficit stressed barley plants confine cell membrane injury? I. Free proline accumulation and membrane injury index in drought and osmotically stressed plants. Acta Physiologiae Plantarum, 22: 409-415. Doi: 10.1007/s11738-000-0081-7

Bandurska H., Cie´ slak M. 2013. The interactive effect of water deficit and UV-B radiation on salicylic acid accumulation in barley roots and leaves. Environmental and Experimental Botany, 94:9-18. Doi: 10.1016/j.envexpbot.2012.03.001

Beaudoin-Eagan L.D., Thorpe T.A. 1985. Tyrosine and phenylalanine ammonia lyase activities during shoot initiation in tobacco callus cultures. Plant Physiology, 78: 438-441. Doi: 10.1104/pp.78.3.438

Benzie F., Strain J. 1996. The ferric reducing ability of plasma (FARP) as a measure of antioxidant power: the FARP assay. Analytical Biochemistry, 239: 70-76. Doi: 10.1006/abio.1996.0292

Bradford M.M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 1151-1154. Doi: 10.1016/0003-2697(76)90527-3

Chang C.C., Yang M.H., Wen H.M., Chern J.C. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10: 178-182.

Chaves M.M., Oliveira M.M. 2004. Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture. Journal of Experimental Botany, 55: 2365-2384. Doi: 10.1093/jxb/erh269

Chen Z.L., Li X.M., Zhang L.H. 2014. Effect of salicylic acid pretreatment on drought stress responses of zoysiagrass (Zoysia japonica). Russian Journal of Plant Physiology, 61: 619-625. Doi: 10.1134/S1021443714050057

Dogbo D.O., Gogbeu S.J., Nzue B., Yao K.A., Zohouri G.P., Mamyrbekovabekro J.A., Bekro Y.A. 2012. Comparative activities of phenylalanine ammonia-lyase and tyrosine ammonia-lyase and phenolic compounds accumulated in cassava elicited cell. African Crop Science Journal, 20: 85-94.

Dubois M., Gilles K.A., Hamilton J.K., Reberts P.A., Smith F. 1956. Colorimetric method for determination of sugar and related substrates. Analytical Chemistry, 28: 350-356. Doi: 10.1021/ac60111a017

Girma F.S., Kreig D.R. 1992. Osmotic adjustment in Sorghum. Plant Physiology 99: 577-582. Doi: 10.1104/pp.99.2.577

Habibi G. 2012. Exogenous salicylic acid alleviates oxidative damage of barley plants under drought stress. Acta Biologica Szegediensis, 56: 57-63.

Hashempour A., Ghasemnezhad M., Fotouhi Ghazvini R., Sohani M.M. 2014. The physiological and biochemical responses to freezing stress of olive plants treated with salicylic acid. Russian Journal of Plant Physiology, 61: 443-450. Doi: 10.1134/S1021443714040098

He Q., Zhao S., Ma Q., Zhang Y., Huang L., Li G., Hao L. 2014. Endogenous salicylic acid levels and signaling positively regulate Arabidopsis response to polyethylene glycol-simulated drought stress. Journal of Plant Growth Regulation, 33: 871-880. Doi: 10.1007/s00344-014-9438-9

Idrees M., Naeem M., Khan M.N., Aftab T., Khan M.M.A., Moinuddin. 2012. Alleviation of salt stress in lemongrass by salicylic acid. Protoplasma, 249: 709-720. Doi: 10.1007/s00709-011-0314-1

Jiménez S., Dridi D., Gutiérrez D., Moret D., Irigoyen J.J., Moreno M.A., Gogorcena Y. 2013. Physiological, biochemical and molecular responses in four Prunus rootstocks submitted to drought stress. Tree Physiology, 33: 1061-1075. Doi: 10.1093/treephys/tpt074

Kabiri R., Nasibi F., Farahbakhsh H. 2014. Effect of exogenous salicylic acid on some physiological parameters and alleviation of drought stress in Nigella sativa plant under hydroponic culture. Plant Protection Science, 50: 43-51.

Kang G.Z., Li G.Z., Liu G.Q., Xu W., Peng X.Q., Wang C.Y., Zhu Y.J, Guo T.C. 2013. Exogenous salicylic acid enhances wheat drought tolerance by influence on the expression of genes related to ascorbate–glutathione cycle. Biologia Plantarum, 57: 718-724. Doi: 10.1007/s10535-013-0335-z

Khokon M.A.R., Okuhama E., Hossain M.A., Uraji S.M.M., Nakamura Y., Murata Y. 2011. Involvement of extracellular oxidative burst in salicylic acid-induced stomatal closure in Arabidopsis. Plant Cell and Eenvironment. 34: 434-443. Doi: 10.1111/j.1365-3040.2010.02253.x

Laby R.J., Kincaid M.S., Kim D., Gibson S.I. 2000. The Arabidopsis sugar-insensitive mutants sis4 and sis5 are defective in abscisic acid synthesis and response. The Plant Journal, 23: 587-596. Doi: 10.1046/j.1365-313x.2000.00833.x

Lichtenthaler H., Wellburn A. 1983. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transactions, 11: 591-592. Doi: 10.1042/bst0110591

Miura K., Tada Y. 2014. Regulation of water, salinity, and cold stress responses by salicylic acid. Frontier in Plant Science, 5: 1-12. Doi: 10.3389/fpls.2014.00004

Mozaffarian V. 2010. Flora of Iran (Composite). Tehran, Iran: Iranian Research Institute of Forest and Rangeland Press.

Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-479. Doi: 10.1111/j.1399-3054.1962.tb08052.x

Nazar R., Umar S., Khan N.A., Sareer O. 2015. Salicylic acid supplementation improves photosynthesis and growth in mustard through changes in proline accumulation and ethylene formation under drought stress. South African Journal of Botany, 98: 84-94. Doi: 10.1016/j.sajb.2015.02.005

Patakas A., Noitsakis B. 2001. Leaf age effects on solute accumulation in water-stressed grapevines. Journal of Plant Physiology, 158: 63-69. Doi: 10.1078/0176-1617-00003

Pinhero R.G., Rao M.V., Palyath G., Murr D.P., Fletcher R.A. 1997. Changes in the activities of antioxidant enzymes and their relationship to genetic and paclobutrazol induced chilling tolerance of maize seedlings. Plant Physiology, 114: 695-704.

Pourcel L., Routaboul J.M., Cheynier V., Lepiniec L., Debeaujon I. 2007. Flavonoid oxidation in plants: from biochemical properties to physiological functions. Trends Plant science, 12: 29-36. Doi: 10.1016/j.tplants.2006.11.006

Rustaiyan A., Bamoniri A., Raffatrad M., Jakupovic J., Bohlman F. 1987. Eudesmanederivatives and highly oxygenatedmonoterpenes from Iranian Artemisia species. Phytochemistry, 26: 2307–2310. Doi: 10.1016/S0031-9422(00)84708-1

Saleh A.M., Madany M.M.Y. 2015. Coumarin pretreatment alleviates salinity stress in wheat seedlings. Plant Physiology and Biochemistry, 88: 27-35. Doi: 10.1016/j.plaphy.2015.01.005

Schroeder A.C., Kumaran S., Hicks L.M., Cahoon R.E., Halls C., Yu O., Jez J.M. 2008. Contributions of conserved serine and tyrosine residues to catalysis, ligand binding, and cofactor processing in the active site of tyrosine ammonia lyase. Phytochemistry, 69: 1496-1506. Doi: 10.1016/j.phytochem.2008.02.007

Shen C., Hu Y., Du X., Li T., Tang H., Wu J. 2014. Salicylic acid induces physiological and biochemical changes in Torreya grandis cv. Merrillii seedlings under drought stress. Trees, 28: 961-970. Doi: 10.1007/s00468-014-1009-y

Siboza X.I., Bertling I., Odindo A.O. 2014. Salicylic acid and methyl jasmonate improve chilling tolerance in cold-stored lemon fruit (Citrus limon). Journal of Plant Physiology, 171: 1722-1731. Doi: 10.1016/j.jplph.2014.05.012

Singh B., Usha K. 2003. Salicylic acid induced physiological and biochemical changes in wheat seedlings under water stress. Plant Growth Regulation, 39:137-141. Doi: 10.1023/A:1022556103536

Singleton V.L., Orthofer R., Lamuela-Raventos R.M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, 299: 152-178. Doi: 10.1016/S0076-6879(99)99017-1

Sutter E., G., 1988. Stomatal and cuticular water loss from apple, cherry, and sweetgum plants after removal from in vitro culture.- Journal of the American Society for Horticultural Science 113:234-238

Sʹwieca M. 2015. Production of ready-to-eat lentil sprouts with improved antioxidant capacity: optimization of elicitation conditions with hydrogen peroxide. Food Chemistry, 180: 219-226

Tamás L., Mistrík I., Alemayehu A., Zelinová V., Boˇcová B., Huttová J. 2015. Salicylic acid alleviates cadmium-induced stress responses through the inhibition of Cd-induced auxin-mediated reactive oxygen species production in barley root tips. Journal of Plant Physiology, 73:1-8. Doi: 10.1016/j.jplph.2014.08.018

Vicente M.R.S., Plasencia J. 2011. Salicylic acid beyond defence: its role in plant growth and development. Journal of Experimental Botany, 62: 3321-3338. Doi: 10.1093/jxb/err031

Weatherly P.E. 1950. Studies in water relation of cotton plants. I. The measurement of water deficits in leaves. New Phytologist, 49: 81–97. Doi: 10.1111/j.1469-8137.1950.tb05146.x



26. 10. 2016



Agronomy section

How to Cite

ABBASPOUR, J., & EHSANPOUR, A. (2016). The impact of salicylic acid on some physiological responses of Artemisia aucheri Boiss. under in vitro drought stress. Acta Agriculturae Slovenica, 107(2), 287-298.

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