Establishment of primary keratinocyte culture from horse tissue biopsates

Authors

  • Jernej OGOREVC Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia
  • Tjaša LAPANJA Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Study program in biotechnology, Groblje 3, SI-1230 Domžale, Slovenia
  • Klavdija POKLUKAR Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Study program in biotechnology, Groblje 3, SI-1230 Domžale, Slovenia
  • Natalija TOMINŠEK Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Study program in biotechnology, Groblje 3, SI-1230 Domžale, Slovenia
  • Peter DOVČ Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia

DOI:

https://doi.org/10.14720/aas.2015.106.2.3

Keywords:

molecular biology, primary cell culture, skin, horses, keratinocytes

Abstract

Primary cell lines established from skin tissue can be used in immunological, proteomic and genomic studies as in vitro skin models. The goal of our study was to establish a primary keratinocyte cell culture from tissue biopsates of two horses. The primary keratinocyte cell culture was obtained by mechanical and enzymatic dissociation and with explant culture method. The result was a heterogeneous primary culture comprised of keratinocytes and fibroblasts. To distinguish epithelial and mesenchymal cells immunofluorescent characterisation was performed, using antibodies against cytokeratin 14 and vimentin. We successfully at attained a primary cell line of keratinocytes, which could potentially be used to study equine skin diseases, as an animal model for human diseases, and for cosmetic and therapeutic product testing.

References

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Published

28. 12. 2015

Issue

Section

Animal Science section

How to Cite

OGOREVC, J., LAPANJA, T., POKLUKAR, K., TOMINŠEK, N., & DOVČ, P. (2015). Establishment of primary keratinocyte culture from horse tissue biopsates. Acta Agriculturae Slovenica, 106(2), 87–91. https://doi.org/10.14720/aas.2015.106.2.3

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