• Vida Škrabanja Biotehniška fakulteta, Oddelek za živilstvo, Jamnikarjeva 101, 1000 Ljubljana, Slovenija



selenium, plant resources, dietary intake, selenium supplementation


Selenium is one of the essential elements that has a direct effect on human health and disease. Environmental conditions and agricultural practice have a profound influence on the selenium content in plants. Cultivation of plants enriched with the selenium has an effective potential for selenium supplementation in diets for population which is exposed to selenium deficiency. Bioavailability of selenium compounds from food is in strong correlation with the source and its chemical form. The selenium of different sources and forms can become a part of human consumption when entering the food chain, wherein the inorganic forms of selenium are metabolized and converted to more available organic forms. Numerous results of systematic research of the selenium content in individual plant species as well as various techniques for producing selenium enriched foodstuffs is reviewed. The soil in Slovenia is selenium-poor and may concern a part of population which is potentially sensitive to selenium status. The merits of selenium effect, either alone or in combination with different environmental changes on plant production published by Slovenian authors are thus closely considered. Controversies continue to prevail regarding adequate amounts for selenium for health and disease prevention. Thus, general and individualized recommendations for selenium intake and supplementation in the future need to be cautiously followed and the reference values continually revised.


Bonafaccia, G., Gambelli, L., Fabjan, N., Kreft, I. (2003). Trace elements in flour and bran from common and tartary buckwheat. Food Chemistry, 83(1), 1-5. Doi: 10.1016/S0308-8146(03)00228-0

Breznik, B., Germ, M., Gaberščik, A., Kreft, I. (2005). Combined effects of elevated UV-B radiation and the addition of selenium on common (Fagopyrum esculentum Moench) and tartary [Fagopyrum tataricum (L.) Gaertn.] buckwheat. Photosynthetica, 43(4), 583-589. Doi: 10.1007/s11099-005-0091-1

Combs, G. F. (2001). Selenium in global food systems. British Journal of Nutrition, 85(5), 517-547. Doi: 10.1079/BJN2000280

Cuderman, P., Ožbolt, L., Kreft, I., Stibilj, V. (2010). Extraction of Se species in buckwheat sprouts grown from seeds soaked in various Se solutions. Food Chemistry, 123(3), 941-948. Doi: 10.1016/j.foodchem.2010.04.063

Cutter, G. A. (1989). Freshwater systems. V Ihnat, M. (ur.), Occurrence and Distribution of Selenium (s. 243–262), Boca Raton, FL: CRC Press Inc.

Deveau, M. (2010). Contribution of drinking water to dietary requirements of essential metals. Journal of Toxicology and Environmental Health. Part A. 73(2), 235-241. Doi: 10.1080/15287390903340880

Edmonds, J. S., Morita, M. (2000). The identification of selenium species in biological samples. Applied Organometallic Chemistry, 14(3), 133-145. Doi: 10.1002/(SICI)1099-0739(200003)14:3<133::AID-AOC966>3.0.CO;2-3

Finley, J. W. (2006). Bioavailability of selenium from foods. Nutrition Reviews, 64(3), 146-151. Doi: 10.1111/j.1753-4887.2006.tb00198.x

Fox, T. E., Atherton, C., Dainty, J. R., Lewis, D. J., Langford, N. J., Baxter, M. J., Crews, H. M., Fairweather-Tait, S. J. (2005). Absorption of selenium from wheat, garlic, and cod intrinsically labeled with Se-77 and Se-82 stable isotopes. International Journal for Vitamin and Nutrition Research, 75(3), 179-186. Doi: 10.1024/0300-9831.75.3.179

Frost, D. V. (1972). The two faces of selenium – can selenophobia be cured? CRC Critical Reviews in Toxicology, 1(4), 467-514. Doi: 10.3109/10408447209103467

Germ, M., Kreft, I., Osvald, J. (2005). Influence of UV-B exclusion and selenium treatment on photochemical efficiency of photosystem II, yield and respiratory potential in pumpkins (Cucurbita pepo L.). Plant Physiology and Biochemistry, 43, 445-448. Doi: 10.1016/j.plaphy.2005.03.004

Germ, M., Kreft, I., Stibilj, V., Osvald, J. (2014). The effect of selenium on yield and primary terminal electron transport system activity in two cultivars of bean plants Phaseolus vulgaris. Acta Biologica Slovenica, 57(2), 3-12.

Germ, M., Kreft, I., Stibilj, V., Urbanc-Berčič, O. (2007a). Combined effects of selenium and drought on photosynthesis and mitochondrial respiration in potato. Plant Physiology and Biochemistry, 45, 162-167. Doi: 10.1016/j.plaphy.2007.01.009

Germ, M., Osvald, J. (2005). Selenium treatment affected respiratory potential in Eruca sativa. Acta Agriculturae Slovenica, 85(2), 329-335.

Germ, M., Stibilj, V., Osvald, J., Kreft, I. (2007b). Effect of selenium foliar application on chicory (Cichorium intybus L.). Journal of Agricultural and Food Chemistry, 55(3), 795-798. Doi: 10.1021/jf0629888

Golob, A., Gadžo, D., Stibilj, V., Djikić, M., Gavrić, T., Germ, M. (2016a). Addition of Se affected concentration of Se in the second generation of tartary buckwheat plants. Folia Biologica et Geologica, 57(1), 87-92.

Golob, A., Gadžo, D., Stibilj, V., Djikić, M., Gavrić, T., Kreft, I., Germ, M. (2016b). Sulphur interferes with selenium accumulation in tartary buckwheat plants. Plant Physiology and Biochemistry, 108, 32-36. Doi: 10.1016/j.plaphy.2016.07.001

Golob, A., Germ, M., Kreft, I., Zelnik, I., Kristan, U., Stibilj, V. (2016c). Selenium uptake and Se compounds in Se-treated buckwheat. Acta Botanica Croatica, 75(1), 17-24. Doi: 10.1515/botcro-2016-0016

Golob, A., Stibilj, V., Kreft, I., Germ, M. (2015). The feasibility of using tartary buckwheat as Se-containing food material. Journal of Chemistry (Hindawi) (Online), 1-4. Doi: 10.1155/2015/246042

Golubkina, N. A., Alfthan, G. V. (1999). The human selenium status in 27 regions of Russia. Journal of Trace Elements in Medicine and Biology, 13(1-2), 15-20. Doi: 10.1016/S0946-672X(99)80018-2

Hatfield, D. L. (ur.), Berry, M. J. (ur.), Gladyshev, N. V. (ur.). (2012). Selenium: Its Molecular Biology and Role in Human Health. 3. izdaja. New York, NY: Springer-Verlag. Doi: 10.1007/978-1-4614-1025-6

Hatfield, D. L. (ur.), Schweizer, U. (ur.), Tsuji, P.A. (ur.), Gladyshev, N. V. (ur.). (2016). Selenium: Its Molecular Biology and Role in Human Health. 4. izdaja. New York, NY: Springer-Verlag. Doi: 10.1007/978-3-319-41283-2

Johnson, C. C., Fordyce, F. M., Rayman, M. P. (2010). Symposium on »Geographical and geological influences on nutrition«: Factors controlling the distribution of selenium in the environment and their impact on health and nutrition. Proceedings of the Nutrition Society, 69(1), 119-32. Doi: 10.1017/S0029665109991807

Kreft, I. (1995). Ajda. Ljubljana, ČZD Kmečki glas.

Kreft., I., Mechora, Š., Germ, M., Stibilj, V. (2013). Impact of selenium on mitochondrial activity in young tartary buckwheat plants. Plant Physiology and Biochemistry, 63, 196-199. Doi: 10.1016/j.plaphy.2012.11.027

Lobinski, R., Edmonds, J., Suzuki, K. T., Uden, P. C. (2000). Species-selective determination of selenium compounds in biological materials (Technical report). Pure and Applied Chemistry, 72(3), 447–461. Doi: 10.1351/pac200072030447

Ngo, D.B., Dikassa, L., Okitolonda, W., Kashala, T. D., Gervy, C., Dumont, J., Vanovervelt, N., Contempré, B., Diplock, A. T., Peach, S., Vanderpas, J. (1997). Selenium status in relation to iodine deficiency. Tropical Medicine and International Health, 2(6), 572-581. Doi: 10.1046/j.1365-3156.1997.d01-326.x

NIJZ. (2014). Opisi kemijskih parametrov, ki jih najdemo v pitni vodi. Nacionalni inštitut za javno zdravje, verzija 2, 29.12.2014, s. 35.

Ožbolt, L., Kreft, S., Kreft, I., Germ, M., Stibilj, V. (2008). Distribution of selenium and phenolics in buckwheat plants grown from seeds soaked in Se solution and under different levels of UV-B radiation. Food Chemistry, 110(3), 691-696. Doi: 10.1016/j.foodchem.2008.02.073

Pirc, S., Šajn, R. (1997). Vloga geokemije v ugotavljanju kemične obremenitve okolja. V Lah, A. (ur.), Kemizacija okolja in življenja - do katere meje? Projekt evropskega leta varstva narave 1995 (s. 165-186). Ljubljana: Slovensko ekološko društvo.

Pokorn, D., Gregorič, B., Poklar, T., Eržen T. (1991). Ocena prehrane v domovih za starejše občane v Ljubljani. Zbornik Biotehniške fakultete Univerze v Ljubljani, 57, 259-271.

Power, R. (1995). Selenium-enriched yeast: form and applications. V Proceedings of the Selenium-Tellurium Development Association 5th International Symposium, Brussels, 8–10 May, (s. 89–94). Grimbergen, Belgium: Selenium–Tellurium Development Association.

Pyrzyńska, K. (2002). Determination of selenium species in environmental samples. Microchimica Acta, 140(1), 55-62. Doi: 10.1007/s00604-001-0899-8

Rayman, M.P. (2004). The use of high-selenium yeast to raise selenium status: how does it measure up? British Journal of Nutrition, 92(4), 557–573. Doi: 10.1079/BJN20041251

Reeves, P. G., Leary, P. D., Gregoire, B. R., Finley, J. W., Lindlauf, J. E., Johnson, L. K. (2005). Selenium bioavailability from buckwheat bran in rats fed a modified AIN-93G torula yeast-based diet. The Journal of Nutrition, 135(11), 2627-2633.

Reilly, C. (2006). Selenium in food and health. 2. izdaja. New York, NY: Springer.

Smrkolj, P., Germ, M., Kreft, I., Stibilj, V. (2006a). Respiratory potential and Se compounds in pea (Pisum sativum L.) plants grown from Se-enriched seeds. Journal of Experimental Botany, 57(14), 3595-3600. Doi: 10.1093/jxb/erl109

Smrkolj, P., Pograjc, L., Hlastan-Ribič, C., Stibilj, V. (2005a). Selenium content in selected Slovenian foodstuffs and estimated daily intakes of selenium. Food Chemistry, 90(4), 691-697. Doi: 10.1016/j.foodchem.2004.04.028

Smrkolj, P., Stibilj, V., Kreft, I., Germ, M., (2006b). Selenium species in buckwheat cultivated with foliar addition of Se(VI) and various levels of UV-B radiation. Food Chemistry, 96(4), 675-681. Doi: 10.1016/j.foodchem.2005.05.002

Smrkolj, P., Stibilj, V., Kreft, I., Kapolna, E. (2005b). Selenium species determination in selenium-enriched pumpkin (Cucurbita pepo L.) seeds by HPLC-UV-HG-AFS. Analytical Sciences, 21(12), 1501-1504. Doi: 10.2116/analsci.21.1501

Smrkolj, P., Žgur, S., Stibilj, V. (2003). Selen v govejem mesu izbranih pasem. Meso in mesnine, 4, 5-8.

Stadlober, M., Sager, M., Irgolic, K. J. (2001). Effects of selenate supplemented fertilisation on the selenium level of cereals - identification and quantification of selenium compounds by HPLC-ICP-MS. Food Chemistry, 73(3), 357-366. Doi: 10.1016/S0308-8146(01)00115-7

Stibilj, V., Kreft, I., Smrkolj, P., Osvald, J. (2004). Enhanced selenium content in buckwheat (Fagopyrum esculentum Moench) and pumpkin (Cucurbita pepo L.) seeds by foliar fertilisation. European Food Research and Technology, 219(2), 142-144. Doi: 10.1007/s00217-004-0927-0

Sun, H., Feng, B. (2011). Speciation of organic and inorganic selenium in selenium-enriched eggs by hydride generation atomic fluorescence spectrometry. Food Analytical Methods, 4(2), 240-244. Doi: 10.1007/s12161-010-9145-5

Tadina, N., Germ, M., Kreft, I., Breznik, B., Gaberščik, A. (2005). Effect of water deficit and selenium on common buckwheat (Fagopyrum esculentum Moench) plants. Photosynthetica, 45(3), 472-476. Doi: 10.1007/s11099-007-0080-7

Tamás, M., Mándoki, Zs., Csapó J. (2010). The role of selenium content of wheat in the human nutrition. A literature review. Acta Universitatis Sapientiae, Alimentaria, 3, 5-34.

Uden, P. C., Boakye, H. T., Kahakachchi. C., Tyson, J. F. (2004). Selective detection and identification of Se containing compounds - review and recent developments. Journal of Chromatography A, 1050(1), 85-93. Doi: 10.1016/j.chroma.2004.05.077

Uradni list RS, št. 19/2004. 1. 3. 2004: 865. Pravilnik o pitni vodi, s. 2155.

Vincenti, M., Bonvicini, F., Bergomi, M., Malagoli, C. (2010). Possible involvement of overexposure to environmental selenium in the etiology of amyotrophic lateral sclerosis: a short review. Annali dell'Istituto Superiore di Sanità, 46, 279-283.

Vogrinčič M., Cuderman, P., Kreft, I., Stibilj, V. (2009). Selenium and its species distribution in above-ground plant parts of selenium enriched buckwheat (Fagopyrum esculentum Moench). Analytical Sciences, 25(11), 1357-1363. Doi: 10.2116/analsci.25.1357

Wang, W. C., Mäkelä, A. L., Näntö, V., Mäkelä, P., Lagström, H. (1998). The serum selenium concentrations in children and young adults: a long-term study during the Finnish selenium fertilization programme. European Journal of Clinical Nutrition, 52(7), 529-535. Doi: 10.1038/sj.ejcn.1600602

Wang, Z. J., Gao, Y. X. (2001). Biogeochemical cycling of selenium in Chinese environments. Applied Geochemistry, 16(11-12), 1345–1351. Doi: 10.1016/S0883-2927(01)00046-4

Watkinson, J. H. (1983). Prevention of selenium deficiency in grazing animals by annual topdressing of pasture with sodium selenate. The New Zealand Veterinary Journal, 31(5), 78-85. Doi: 10.1080/00480169.1983.34975

Wolf, W. R., Goldschmidt, R. J. (2007). Updated estimates of the selenomethionine content of NIST wheat reference materials by GC-IDMS. Analytical and Bioanalytical Chemistry, 387(7), 2449-2452. Doi: 10.1007/s00216-006-0839-x

Zhao, C., Roseland, J., Andrews, K.W., Feinberg, M., Middleton, A., Holden, J., Douglass, L., Dwyer, J., Picciano, M.F. (2009). Levels of the Antioxidant Nutrients, Vitamin C, Vitamin E, and Selenium in the Dietary Supplement Ingredient Database: NHANES Data Applications. American Institute of Cancer Research Annual Research Conference, November 5-6, 2009, Washington, DC.


24. 01. 2017



Agronomy section

How to Cite

Škrabanja, V. (2017). PLANT RESOURCES BASED SELENIUM SUPPLEMENTATION IN DAILY NUTRITION. Acta Agriculturae Slovenica, 109(1), 147-155.

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