SUSCEPTIBILITY OF SPOTTED WING DROSOPHILA (Drosophila suzukii (Matsumura, 1931)) PUPAE TO ENTOMOPATHOGENIC FUNGI

Authors

  • Jaka Razinger Oddelek za varstvo rastlin, Kmetijski inštitut Slovenije, Hacquetova ulica 17, 1000 Ljubljana, Slovenija
  • Katja FINK univ. dipl. prof. biol. kem, Slovenia
  • Ana KERIN univ. dipl. biol., Slovenia
  • Špela MODIC Oddelek za varstvo rastlin, Kmetijski inštitut Slovenije, Hacquetova ulica 17, 1000 Ljubljana, Slovenija
  • Gregor UREK Oddelek za varstvo rastlin, Kmetijski inštitut Slovenije, Hacquetova ulica 17, 1000 Ljubljana, Slovenija

DOI:

https://doi.org/10.14720/aas.2017.109.1.12

Keywords:

biological control, entomopathogenic fungi, insect-pathogenic soil fungi, insect, organic, pest, soft fruit, virulence

Abstract

Spotted wing drosophila (Drosophila suzukii (Matsumura, 1931), Diptera, Drosophilidae) management is difficult mainly because of its short generation time, polyphagy and serrated ovipositor, but also because its larvae can pupate in the orchard soil and are thus protected from insecticide applications. We hypothesized that insect-pathogenic soil fungi could successfully infect Drosophila suzukii pupae in soil environment. We tested several entomopathogenic or soil fungi against pupae in a) conidia-spiked soil, b) via direct applications of conidia, and c) by dipping pupae into conidial suspensions. Metarhizium brunneum Petch strain H.J.S. 1154 significantly reduced fly emergence in conidia spiked soil and bioinsecticide Naturalis (based on entomopathogenic fungus Beauveria bassiana (Bals.-Criv.) Vuill. in direct exposure tests. Our attempt to determine IC50 of pupal hatching rate by dipping D. suzukii pupae into conidial suspensions was unsuccessful. We conclude that the pupal stage is probably too brief to allow entomopathogens to cause a significant reduction of fly emergence. According to our results and published articles, the fungal biocontrol potential would probably best be evaluated in spray applications against adult flies.

Author Biography

  • Jaka Razinger, Oddelek za varstvo rastlin, Kmetijski inštitut Slovenije, Hacquetova ulica 17, 1000 Ljubljana, Slovenija

    raziskovalec

    Oddelek za varstvo rastlin

    Skupina za entomologijo

References

Bohinc, T., Trdan, S. 2014. Zatiranje plodove vinske mušice (Drosophila suzukii [Matsumura], Diptera, Drosophilidae) s poudarkom na okoljsko sprejemljivih načinih. Acta agriculturae Slovenica, 103, 2: 323-329. Doi: 10.14720/aas.2014.103.2.17

Bruck, D. J., Bolda, M., Tanigoshi, L., Klick, J., Kleiber, J., DeFrancesco, J., Spitler, H. (2011). Laboratory and field comparisons of insecticides to reduce infestation of Drosophila suzukii in berry crops. Pest Management Science, 67(11), 1375–85. Doi: 10.1002/ps.2242

Chabert, S., Allemand, R., Poyet, M., Eslin, P., & Gibert, P. (2012). Ability of European parasitoids (Hymenoptera) to control a new invasive Asiatic pest, Drosophila suzukii. Biological Control, 63(1), 40–47. Doi: 10.1016/j.biocontrol.2012.05.005

Cini, A., Anfora, G., Escudero-Colomar, L. A., Grassi, A., Santosuosso, U., Seljak, G., & Papini, A. (2014). Tracking the invasion of the alien fruit pest Drosophila suzukii in Europe. Journal of Pest Science, 87, 559–566. Doi: 10.1007/s10340-014-0617-z

Cini, A., Ioriatti, C., & Anfora, G. (2012). A review of the invasion of Drosophila suzukii in Europe and a draft research agenda for integrated pest management. Bulletin of Insectology, 65(1), 149–160.

Cuthbertson, A. G. S., Blackburn, L. F., & Audsley, N. (2014). Efficacy of Commercially Available Invertebrate Predators against Drosophila suzukii. Insects, 5(4), 952–960. Doi: 10.3390/insects5040952

Cuthbertson, A. G. S., Collins, D. A., Blackburn, L. F., Audsley, N., & Bell, H. A. (2014). Preliminary screening of potential control products against Drosophila suzukii. Insects, 5(2), 488–498. Doi: 10.3390/insects5020488

Gabarra, R., Riudavets, J., Rodríguez, G. a., Pujade-Villar, J., & Arnó, J. (2015). Prospects for the biological control of Drosophila suzukii. BioControl, 60(3), 331–339. Doi: 10.1007/s10526-014-9646-z

Gaddum, J. H. (1948). Probit Analysis. Nature, 161(4090), 417–418. Doi: 10.1038/161417a0

Gargani, E., Tarchi, F., Frosinini, R., Mazza, G., & Simoni, S. (2013). Notes on Drosophila suzukii Matsumura (Diptera Drosophilidae): field survey in Tuscany and laboratory evaluation of organic products. Redia. Retrieved from http://cra-journals.cineca.it/index.php/redia/article/view/769

Lee, J. C., Bruck, D. J., Dreves, A. J., Ioriatti, C., Vogt, H., & Baufeld, P. (2011). In Focus: Spotted wing drosophila, Drosophila suzukii, across perspectives. Pest Management Science, 67(11), 1349–1351. Doi: 10.1002/ps.2271

Motulsky, H. (1995). Intuitive biostatistics. Oxford: Oxford University Press.

Naranjo-Lázaro, J. M., Mellín-Rosas, M. A., González-Padilla, V. D., Sánchez-González, J. A., Moreno-Carrillo, G., & Arredondo-Bernal, H. C. (2014). Susceptibility of Drosophila suzukii Matsumura (Diptera: Drosophilidae) to Entomophatogenic Fungi. Southwestern Entomologist, 39(1), 201–203. Doi: 10.3958/059.039.0119

Razinger, J., Lutz, M., Schroers, H. J., Palmisano, M., Wohler, C., Urek, G., & Grunder, J. (2014b). Direct plantlet inoculation with soil or insect-associated fungi may control cabbage root fly maggots. Journal of Invertebrate Pathology, 120, 59–66. Doi: 10.1016/j.jip.2014.05.006

Razinger, J., Lutz, M., Schroers, H.-J., Urek, G., & Grunder, J. (2014a). Evaluation of Insect Associated and Plant Growth Promoting Fungi in the Control of Cabbage Root Flies. Journal of Economic Entomology, 107(4), 1348–1354. Journal Article. Doi: 10.1603/EC14004

Rossi Stacconi, M. V., Buffington, M., Daane, K. M., Dalton, D. T., Grassi, A., Kaçar, G., Anfora, G. (2015). Host stage preference, efficacy and fecundity of parasitoids attacking Drosophila suzukii in newly invaded areas. Biological Control, 84, 28–35. Doi: 10.1016/j.biocontrol.2015.02.003

Rota-Stabelli, O., Blaxter, M., & Anfora, G. (2013). Drosophila suzukii. Current Biology, 23(1), R8–R9. Doi: 10.1016/j.cub.2012.11.021

Van Timmeren, S., & Isaacs, R. (2013). Control of spotted wing drosophila, Drosophila suzukii, by specific insecticides and by conventional and organic crop protection programs. Crop Protection, 54, 126–133. Doi: 10.1016/j.cropro.2013.08.003

Walse, S. S., Krugner, R., & Tebbets, J. S. (2012). Postharvest treatment of strawberries with methyl bromide to control spotted wing drosophila, Drosophila suzukii. Journal of Asia-Pacific Entomology, 15(3), 451–456. Doi: 10.1016/j.aspen.2012.05.003

Woltz, J. M., Donahue, K. M., Bruck, D. J., & Lee, J. C. (2015). Efficacy of commercially available predators, nematodes and fungal entomopathogens for augmentative control of Drosophila suzukii. Journal of Applied Entomology, n/a-n/a. Doi: 10.1111/jen.12200

Published

24. 01. 2017

Issue

Section

Agronomy section

How to Cite

Razinger, J., FINK, K., KERIN, A., MODIC, Špela, & UREK, G. (2017). SUSCEPTIBILITY OF SPOTTED WING DROSOPHILA (Drosophila suzukii (Matsumura, 1931)) PUPAE TO ENTOMOPATHOGENIC FUNGI. Acta Agriculturae Slovenica, 109(1), 125-134. https://doi.org/10.14720/aas.2017.109.1.12

Similar Articles

1-10 of 678

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)

1 2 3 > >>