First report of nematodes Parasitylenchus bifurcatus Poinar & Steenberg, 2012 parasitizing multicolored Asian lady beetle Harmonia axyridis (Pallas, 1773) in Slovenia

Authors

  • Barbara Gerič Stare Kmetijski inštitut Slovenije, Slovenija
  • Saša ŠIRCA Kmetijski inštitut Slovenije, Slovenija
  • Gregor UREK Kmetijski inštitut Slovenije, Slovenija

DOI:

https://doi.org/10.14720/aas.2017.109.2.28

Keywords:

parasitic nematodes, ladybirds beetles, Parasitylenchus bifurcatus, Harmonia axyridis, harlequin ladybird, biological control

Abstract

In years 2015 and 2016 around 200 specimens of ladybird species Harmonia axyridis were collected in Slovenia. Ladybirds were dissected and checked under stereomicroscope for presence of nematodes. Nematodes were discovered in samples of ladybirds H. axyridis collected at Brdo pri Lukovici and Trboje. The incidence of parasitized ladybirds was around 30 %. Nematodes were analysed morphometrically and the characteristic features of Parasitylenchus bifurcatus nematodes were observed: straight stylet lacking basal thickenings, a bursa and a forked tail tip in the vermiform females and juvenile males. The identity was confirmed with 18S rDNA region sequence (acc. no. LT629306 and LT629307) which showed high similarity (>99.9 % nucleotide identity) to the P. bifurcatus sequences in the public domain. This is a first report of P. bifurcatus nematode species found parasitizing ladybird Harmonia axyridis in Slovenia. The species compromises fecundity of its host and has therefore a potential to be used as a biological control agent to control high abundance of invasive H. axyridis ladybirds.

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Published

26. 09. 2017

Issue

Section

Agronomy section

How to Cite

Gerič Stare, B., ŠIRCA, S., & UREK, G. (2017). First report of nematodes Parasitylenchus bifurcatus Poinar & Steenberg, 2012 parasitizing multicolored Asian lady beetle Harmonia axyridis (Pallas, 1773) in Slovenia. Acta Agriculturae Slovenica, 109(2), 457–463. https://doi.org/10.14720/aas.2017.109.2.28

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