CUPRAC assay-guided profiling of antioxidant compounds in methanol extract of Lentinus squarrosulus Mont. mycelium

Sumaiyah ABDULLAH; Noorlidah ABDULLAH; Vikineswary SABARATNAM; Ken Choy YAP

doi:10.14720/aas.2018.111.1.10

Authors

Sumaiyah ABDULLAH Universiti Putra Malaysia, Malaysia
Noorlidah ABDULLAH University of Malaya, Malaysia
Vikineswary SABARATNAM University of Malaya, Malaysia
Ken Choy YAP Advanced Chemical Solution, Malaysia

DOI:

https://doi.org/10.14720/aas.2018.111.1.10

Keywords:

medicinal mushroom, active compounds, Lentinus squarrosulus

Abstract

A cupric reducing antioxidant capacity (CUPRAC)-guided purification approach was performed on a methanol extract of Lentinus squarrosulus (LsqMeOH) by using reversed phase-high performance liquid chromatography. Using reversed phase-high performance liquid chromatography, three fractions were separated arbitrarily named FR1, FR2 and FR3. Results showed that FR2 exhibited the highest antioxidant activity in CUPRAC assay (A₄₅₀, 0.86) but not significantly different from LsqMeOH (A₄₅₀, 0.84). FR1 and FR3 showed much lower absorbance, with values (A₄₅₀, 0.21) and (A₄₅₀, 0.36) respectively at 1 mg ml^-1. The most active fraction (F3) was further subjected to LC-MS/MS to obtain its detailed chemical profile. Uridine, ganoderic acid derivative, and flavonoids were the first time being found in L. squarrosulus antioxidative fractions. The present results indicate that the fraction extracts of L. squarrosulus possess antioxidant properties and can be used as free radical inhibitors. Therefore, this research suggested the potentials of L. squarrosulus as a source of antioxidant extract to be used in food industries (functional food).

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