Physiological and biochemical changes in Matricaria chamomilla induced by Pseudomonas fluorescens and water deficit stress

Authors

  • Hamid MOHAMMADI Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran
  • Mohammad ESMAILPOUR Department of Medicinal and Aromatic plants, Jahrom Universtiy, Jahrom, Iran.
  • Samaneh GHORBI Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran
  • Mehrnaz HATAMI Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran

DOI:

https://doi.org/10.14720/aas.2018.111.1.07

Keywords:

PGPR, chamomile, essential oil, injury indices, water deficit stress

Abstract

Environmental stresses and rhizosphere microorganisms affect growth parameters and accumulation of active ingredients especially in plants with medicinal properties. The present study examined the effects of chamomile (Matricaria chamomilla L.) seedling inoculation with Pseudomonas fluorescens PF-135 strain on its growth parameters, photosynthetic pigments, proline, malondialdehyde (MDA), and hydrogen peroxide (H2O2) content, and essential oil concentration at both regular watering and water deficit experiments. Based on the obtained results, water deficit stress reduced root dry mass, and flower fresh and dry mass as well. However, amount of H2O2 and MDA in root and shoot tissues were considerably lower in inoculated plants compared to non-inoculated ones under both normal watering and water deficit regimes. It indicates that lipid peroxidation and production of reactive oxygen species has been diminished in inoculated plants. Also, essential oil content in inoculated plants significantly increased compared with that of non-inoculated ones under water deficit stress condition. It can be concluded that P. fluorescens PF-135 strain has an outstanding potential to alleviate adverse effects of water deficit on plant growth, and hence can be used as an excellent PGPR in order to boost chamomile productivity especially under water deficit stress condition.

References

Ashraf, M., & Foolad, M.R. (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany, 59, 206-216. https://doi.org/10.1016/j.envexpbot.2005.12.006

Bates, L., Waldren, R. & Teare, I. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39, 205-207. https://doi.org/10.1007/BF00018060

Charousaei, F., Dabirian, A. & Mojab, F. (2011). Using chamomile solution or a 1% topical hydrocortisone ointment in the management of peristomal skin lesions in colostomy patients: results of a controlled clinical study. Ostomy-Wound Management, 57, 28-36.

Chauhan, H., Bagyaraj, D.J., Selvakumar, G. & Sundaram, S.P. (2015). Novel plant growth promoting rhizobacteria—Prospects and potential. Applied Soil Ecology, 95, 38–53. https://doi.org/10.1016/j.apsoil.2015.05.011

Christians, M.J., Gingerich, D.J., Hansen, M., Binder, B.M., Kieber, J.J. & Vierstra, R.D. (2009). The BTB ubiquitin ligases ETO1, EOL1 and EOL2 act collectively to regulate ethylene biosynthesis in Arabidopsis by controlling type-2 ACC synthase levels. The Plant Journal, 57, 332-345. https://doi.org/10.1111/j.1365-313X.2008.03693.x

Cruz de Carvalho, M.H. (2008). Drought stress and reactive oxygen species: production, scavenging and signaling. Plant Signaling & Behavior, 3, 156-165. https://doi.org/10.4161/psb.3.3.5536

Farooq, M., Wahid, A., Kobayashi, N., Fujita, D. & Basra, S. (2009). Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development, 29, 185-212. https://doi.org/10.1051/agro:2008021

Gill, S.S. & Tuteja, N. (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48, 909-930. https://doi.org/10.1016/j.plaphy.2010.08.016

Glick, B.R. (1995). The enhancement of plant growth by free-living bacteria. Canadian Journal of Microbiology, 41, 109-117. https://doi.org/10.1139/m95-015

Glick, W.H., Miller, C.C. & Cardinal, L.B. (2007). Making a life in the field of organization science. Journal of Organizational Behavior, 28, 817-835. https://doi.org/10.1002/job.455

Glick, B.R. (2014). Bacteria with ACC deaminase can promote plant growth and help to feed the world. Microbiological Research, 169(1), 30-39. https://doi.org/10.1016/j.micres.2013.09.009

Ghorbanpour, M., Hatami, M., Kariman, K. & Khavazi, K. (2015). Enhanced Efficiency of Medicinal and Aromatic Plants by PGPRs. In: Plant-Growth-Promoting Rhizobacteria (PGPR) and Medicinal Plants, pp. 43-70. E. Dilfuza, Sh. Smriti, V. Ajit (eds.). Springer International Publishing. Switzerland. https://doi.org/10.1007/978-3-319-13401-7_3

Ghorbanpour, M., Hatami, M., Kariman, K. & Abbaszadeh, P. (2016). Phytochemical variations and enhanced efficiency of antioxidant and antimicrobial ingredients in Salvia officinalis as inoculated with different rhizobacteria. Chemistry & Biodiversity, 13, 319-330. https://doi.org/10.1002/cbdv.201500082

Heath, R.L. & Packer, L. (1968). Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125, 189-198. https://doi.org/10.1016/0003-9861(68)90654-1

Letchamo, W. (1993). Nitrogen application affects yield and content of the active substances in chamomile genotypes. New Crops. Willey. New York, 636-639.

Lichtenthaler, H.K., Wellburn, A.R. (1983). Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transactions, 11, 591-592. https://doi.org/10.1042/bst0110591

Maggio, A., Miyazaki, S., Veronese, P., Fujita, T., Ibeas, J.I., Damsz, B., Narasimhan, M.L., Hasegawa, P.M., Joly, R.J. & Bressan, R.A. (2002). Does proline accumulation play an active role in stress‐induced growth reduction? The Plant Journal, 31, 699-712. https://doi.org/10.1046/j.1365-313X.2002.01389.x

Mehrabi, Z., McMillan, V.E., Clark, I.M., Canning, G., Hammond-Kosack, K.E., Preston, G., Hirsch, P.R. & Mauchline, T.H. (2016). Pseudomonas spp. diversity is negatively associated with suppression of the wheat take-all pathogen. Scientific. Reports, 6, 1-9. https://doi.org/10.1038/srep29905

Mittler, R. (2002). Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science, 7, 405-410. https://doi.org/10.1016/S1360-1385(02)02312-9

Mohammadi, H., Dashi, R., Farzaneh, M., Parviz, L., & Hashempour, H. (2017). Effects of beneficial root pseudomonas on morphological, physiological, and phytochemical characteristics of Satureja hortensis (Lamiaceae) under water stress. Brazilian Journal of Botany, 40(1), 41-48. https://doi.org/10.1007/s40415-016-0319-2

Roby, M.H.H., Sarhan, M.A., Selim, K.A.H. & Khalel, K.I. (2013). Antioxidant and antimicrobial activities of essential oil and extracts of fennel (Foeniculum vulgare L.) and chamomile (Matricaria chamomilla L.). Industrial Crops and Products, 44, 437-445. https://doi.org/10.1016/j.indcrop.2012.10.012

Saleem, M., Arshad, M., Hussain, S. & Bhatti, A.S. (2007). Perspective of plant growth promoting rhizobacteria (PGPR) containing ACC deaminase in stress agriculture. Journal of Industrial Microbiology & Biotechnology, 34, 635–648. https://doi.org/10.1007/s10295-007-0240-6

Sánchez-Blanco, M.J., Ferrández, T., Morales, M.A., Morte, A. & Alarcón, J.J. (2004). Variations in water status, gas exchange, and growth in Rosmarinus officinalis plants infected with Glomus deserticola under drought conditions. Journal of Plant Physiology, 161, 675-682. https://doi.org/10.1078/0176-1617-01191

Downloads

Published

8. 04. 2018

Issue

Section

Agronomy section

How to Cite

MOHAMMADI, H., ESMAILPOUR, M., GHORBI, S., & HATAMI, M. (2018). Physiological and biochemical changes in Matricaria chamomilla induced by Pseudomonas fluorescens and water deficit stress. Acta Agriculturae Slovenica, 111(1), 63–72. https://doi.org/10.14720/aas.2018.111.1.07