Detection and characterization of endophytic bacteria causing knot in young olive trees

Authors

  • Maryam KHEZRI Assistant Professor of Plant Pathogenic Prokaryotes, Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia, Iran
  • Abolghasem GHASEMI
  • Akbar AHANGARAN

DOI:

https://doi.org/10.14720/aas.2019.113.1.10

Keywords:

Pseudomonas savastanoi pv. savastanoi, Phenotypic identification, 16S rRNA, rpoB, cultivar resistance

Abstract

Olive knot is an important disease in most countries where olives are commercially grown. In the spring of 2015, some galls were observed on the trunk and branches of 4-year-old olive trees in the north of Iran. The bacteria were isolated from galls and all isolates were gram-negative, aerobic, and capable of producing florescent pigment. Other phenotypic characteristics of the isolates were assessed. Pathogenicity tests were carried out on olive branches incubated with different isolates. Primary symptoms were observed after two weeks. Sequences of 16S rRNA and RNA polymerase beta subunit genes of pathogenic isolates were completely similar to Pseudomonas savastanoi pv. savastanoi (Smith 1908) Young et al. 1978 in GenBank. Based on the results from phenotypic analyses, pathogenicity tests and phylogenetic data, the isolates were identified as P. savastanoi pv. savastanoi. The host range of our isolates was specific to olive trees. None of the inoculated oleander (Nerium oleander L.), winter jasmine (Jasminum nudiflorum Lindl.), Japanese privet (Ligustrum japonicum Thunb.) and ash (Fraxinus excelsior L.) developed disease symptoms. No difference in disease resistance was observed between six studied olive cultivars. There was no olive tree or orchard around the studied orchard as far as more than one kilometer. As the disease agent listed in Iran’s foreign quarantine pests and diseases list, appropriate quarantine and phytosanitary measures were undertaken to eradicate the disease.

Author Biographies

  • Maryam KHEZRI, Assistant Professor of Plant Pathogenic Prokaryotes, Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia, Iran
    Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia, Iran
  • Abolghasem GHASEMI
    Department of Plant Pathology Research, Iranian Research Institute of Plant Protection (IRIPP), Tehran, Iran
  • Akbar AHANGARAN
    Director General, Bureau of Plant Quarantine, Plant Protection Organization (PPO), Tehran, Iran.

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Published

1. 04. 2019

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Agronomy section

How to Cite

KHEZRI, M., GHASEMI, A., & AHANGARAN, A. (2019). Detection and characterization of endophytic bacteria causing knot in young olive trees. Acta Agriculturae Slovenica, 113(1), 109–119. https://doi.org/10.14720/aas.2019.113.1.10

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