Some important aspects in Moringa oleifera Lam. micropropagation


  • Abdullah EL-NAGISH Botany and Microbiology Department, Faculty of Science, Sohag University, 82524 Sohag, Egypt
  • Ahmed HASSANEIN Botany and Microbiology Department, Faculty of Science, Sohag University, 82524 Sohag, Egypt
  • Jehan SALEM Botany and Microbiology Department, Faculty of Science, Sohag University, 82524 Sohag, Egypt
  • Fayza FAHEED Botany and Microbiology Department, Faculty of Science, Sohag University, 82524 Sohag, Egypt



medium type, shoot multiplication, cytokinins, gene expression, isoenzymes, micropropagation, antioxidant enzymes, type of explant


Type and source of explant as well as the type of cytokinin were important factors for successful moringa micropropagation. Explants obtained from in vitro grown plant materials were better than others obtained from soil growing seedlings. In addition, nodal segments were better than shoot cuttings in terms of number of shoots/ explants, frequency of shoot formation and number of nodes/shoot. While callus formation on the base of nodal segment on BAP containing media were higher than those of KIN, especially under the influence of high concentration as an aspect of vitrification, BAP was better than KIN in moringa multiplication. Low nutrient medium (half strength MS) supplemented with 0.5 mg l-1 IAA was essential for successful root formation. The suitable conditions for moringa micropropagation on full strength MS or SH may exert low stress and low need to raise the expression of SOD and POX. On the other side, stress due to over increase of chemical components of double MS medium or low nutrient content of half strength MS, B5 or WPM expressed the highest number and staining intensity of SOD and POX bands, vice versa was detected in case of CAT.

Author Biography

  • Abdullah EL-NAGISH, Botany and Microbiology Department, Faculty of Science, Sohag University, 82524 Sohag, Egypt

    Assistant lecturer at Botany and Microbiology Department, Faculty of Science, Sohag University, Sohag, Egypt


Anwar, F., & Bhanger, M. I. (2003). Analytical characterization of Moringa oleifera seed oil grown in temperate regions of Pakistan. Journal of Agricultural and food Chemistry, 51(22), 6558-6563.

Beauchamp, C., & Fridovich, I. (1971). Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical biochemistry, 44(1), 276-287.

Bhatia, S., Othman, Z., & Ahmad, A. L. (2007). Coagulation–flocculation process for POME treatment using Moringa oleifera seeds extract: optimization studies. Chemical Engineering Journal, 133(1), 205-212.

Bhatt, I. D., & Dhar, U. (2004). Factors controlling micropropagation of Myrica esculenta buch.–Ham. ex D. Don: a high value wild edible of Kumaun Himalaya. African Journal of Biotechnology, 3(10), 534-540.

Brewer, G. J., & Sing, C. F. (1970). Introduction to isozyme techniques.

Caceres, A., Saravia, A., Rizzo, S., Zabala, L., De Leon, E., & Nave, F. (1992). Pharmacologie properties of Moringa oleifera. 2: Screening for antispasmodic, antiinflammatory and diuretic activity. Journal of Ethnopharmacology, 36(3), 233-237.

Chen, J., & Ziv, M. (2001). The effect of ancymidol on hyperhydricity, regeneration, starch and antioxidant enzymatic activities in liquidcultured Narcissus. Plant Cell Reports, 20. 22–27.

Church World Service. 2000. Moringa oleifera-the miracle tree. Church World Service. 3.

Classic Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-97.

Cohen, D. (1994). The culture medium. Environmental Effects and their Control in Plant Tissue Culture 393, 15-24.

Devi, P., Satyanarayana, B., Arundhati, A., Rao, T. (2013). Activity of antioxidant enzymes and secondary metabolites during in vitro regeneration of Sterculia urens. Biologia Plantarum, 57, 778–782.

Dillard, C. J., & German, J. B. (2000). Phytochemicals: nutraceuticals and human health. Journal of the Science of Food and Agriculture, 80(12), 1744-1756.<1744::AID-JSFA725>3.0.CO;2-W

Fahey, J. W. (2005). Moringa oleifera: A Review of the Medical Evidence for Its Nutritional, Therapeutic, and Prophylactic Properties. Part 1. Trees for life Journal, 1(5).

Förster, N., Mewis, I., & Ulrichs, C. (2013). Moringa Oleifera—Establishment and Multiplication of Different Ecotypes In Vitro. Gesunde Pflanzen, 65(1), 21-31.

Gamborg, O. L., Miller, R., & Ojima, K. (1968). Nutrient requirements of suspension cultures of soybean root cells. Experimental cell research, 50(1), 151-158.

George, E. F. (1993). Plant propagation by tissue culture. Part 1: the technology (No. Ed. 2). Exegetics limited.

Gnamien, Y. G., Bi, Z., Arsène, I., Kouadio, Y. J., Brostaux, Y., & Baudoin, J. P. (2013). Medium effects on micropropagation and genetic stability of Citrullus lanatus oleaginous type. Agricultural Sciences, 4(07), 32-44.

Guevara, A. P., Vargas, C., Sakurai, H., Fujiwara, Y., Hashimoto, K., Maoka, T., ... & Nishino, H. (1999). An antitumor promoter from Moringa oleifera Lam. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 440(2), 181-188.

Hassanein, A. M., Ahmed, A. M., & Soltan, D. M. (2008). Study of somaclonal variation and gene expression as influenced by long term culture in sorghum. Current Opinion in Biotechnology, 4, 13-20.

Hassanein, A. M., El-Sherbeeny, G. R., Kalid, A. S. G., & Gaboor, G. M. (2015). Seed propagation increases genetic variation and micropropagation to multiply selected shrub with desirable characters. Journal of International Scientific Publications, 3, 325-339.

Hassanein, A.M., Salem, J.M., Faheed, F.A., & El-nagish A. (2018). Effect of anti-ethylene compounds on isoenzyme patterns and genome stability during long term culture of Moringa oleifera. Plant Cell ,Tissue and Organ Cultutre, 132(1), 201-212.

Ibrahim, M. A., Al-Taha, H., & Seheem, A. A. (2013). Effect of cytokinin type and concentration, and source of explant on shoot multiplication of pineapple plant (Ananas comosus' Queen') in vitro/Ucinek vrst in koncentracij citokininov ter vira stebelnih izseckov na in vitro razmnozevanje ananasa (Ananas comosus' Queen'). Acta agriculturae Slovenica, 101(1), 15.

Islam, S., Jahan, M. A. A., & Khatun, R. (2005). In vitro regeneration and multiplication of year-round fruit bearing Moringa oleifera L. Journal of Biological Sciences , 5, 145-148.

Kevers, C., Franck, T., Strasser, R. J., Dommes, J., & Gaspar, T. (2004). Hyperhydricity of micropropagated shoots: a typically stress-induced change of physiological state. Plant Cell, Tissue and Organ Culture, 77(2), 181-191.

Kumar, N., & Reddy, M. P. (2011). In vitro plant propagation: a review. Journal of Forest and Environmental Science, 27(2), 61-72.

Lloyd, G., & McCown, B. (1980). Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Combined Proceedings. International Plant Propagators’ Society, 30, 421-427.

McCown, B. H., & Sellmer, J. C. (1987). General media and vessels suitable for woody plant culture. In: Cell and tissue culture in forestry (pp. 4-16). Springer Netherlands.

Mirzai, F., Uliaie, E.D., & Hagh, A.B. (2015). Stimulation Effect of AgNO3 and CoCl2 as Ethylene Inhibitors on in-Vitro Organogenesis of Sunflower (Helianthus annuus L.). Journal of Agricultural Science, 25(2), 113-118.

Morton, J. F. (1991). The horseradish tree, Moringa pterygosperma (Moringaceae)—a boon to arid lands?. Economic botany, 45(3), 318-333.

Murakami, A., Kitazono, Y., Jiwajinda, S., Koshimizu, K., & Ohigashi, H. (1998). Niaziminin, a thiocarbamate from the leaves of Moringa oleifera, holds a strict structural requirement for inhibition of tumor-promoter-induced Epstein-Barr virus activation. Planta Medica, 64(04), 319-323.

Ndabigengesere, A., & Narasiah, K. S. (1998). Quality of water treated by coagulation using Moringa oleifera seeds. Water research, 32(3), 781-791.

Parzymies, M., & Dąbski, M. (2012). The effect of cytokinin types and their concentration on in vitro multiplication of Clematis viticella (L.) and Clematis integrifolia ‘Petit Faucon’. Acta Scientiarum . Polonorum, Hortorum Cultus, 11(1), 81-91.

Pawar, U. R., & Panneerselvam, R. (2012). Changes of protein content, activity and active isoforms of antioxidative enzymes in Excoecaria agallocha under salt stress. International Journal of Research in Plant Science, 2(4), 62-66.

Pierik, R. L. M. (1997). In vitro culture of higher plants. Springer Science & Business Media.

Reddy, M. P., Kumar, N., Vijay Anand, K. G., Singh, A. H., & Singh, S. (2008). Method for micropropagation of Jatropha curcas plants from leaf explants. Patent filed US and PCT, Application, (2537).

Riyathong, T., Dheeranupattana, S., Palee, J., & Shank, L. (2010). Shoot Multiplication and Plant Regeneration from In vitro cultures of drumstick tree (Moringa oleifera Lam.). In: Proceedings of the 8th International Symposium on Biocontrol and Biotechnology. King Mongkut’s Institute of Technology Ladkrabang and Khon Kaen University, Nongkhai Campus, Thailand, 99–104

Rojas-Martínez, L., Visser, R. G., & de Klerk, G. J. (2010). The hyperhydricity syndrome: waterlogging of plant tissues as a major cause. Propagation of Ornamental Plants, 10(4), 169-175.

Rout, J., Ram, S., Das, R., Chakraboty, A., Sudarshan, M., & Sahoo, S. (2013). Copper-stress induced alterations in protein profile and antioxidant enzymes activities in the in vitro grown Withania somnifera L. Physiology and Molecular Biology of Plants, 19(3),353–361.

Salem, J. M. (2016). In vitro propagation of Moringa oleifera L. under salinity and ventilation conditions. Genetics and Plant Physiology, 6(1-2), 54-64.

Sánchez, N. R., Ledin, S., & Ledin, I. (2006). Biomass production and chemical composition of Moringa oleifera under different management regimes in Nicaragua. Agroforestry Systems, 66(3), 231-242.

Sauer, A., Walther, F., & Preil, W. (1985). Different Suitability for in vitro Propagation of Rose Cultivars/Sortentypische Eignung von Rosen für in vitro Vermehrung. Gartenbauwissenschaft, 133-138.

Schenk, R. U., & Hildebrandt, A. C. (1972). Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Canadian Journal of Botany, 50(1), 199-204.

Sharma, G. K., & Raina, V. (1982). Propagation techniques of Moringa oleifera Lam. In: Improvement of forest biomass: symposium proceedings/edited by PK Khosla. Solan, India: Indian Society of Tree Scientists, c1982.

Siddhuraju, P., & Becker, K. (2003). Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. Journal of agricultural and food chemistry, 51(8), 2144-2155.

Siegel, B. Z., & Galston, A. W. (1967). The isoperoxidases of Pisum sativum. Plant Physiology, 42(2), 221-226.

Snedecor, G.W., & Ochran, W.G. (1980). Statistical methods. Oxford and J. B. H. Publishing Com. 7th. In: I. A. Ames (ed.). - Iowa State University, 166-190.

Stephenson, K. K., & Fahey, J. W. (2004). Development of tissue culture methods for the rescue and propagation of endangered Moringa spp. germplasm. Economic botany, 58(sp1), 116-124.[S116:DOTCMF]2.0.CO;2

Suarez, M., Entenza, J. M., Doerries, C., Meyer, E., Bourquin, L., Sutherland, J., ... & Mermod, N. (2003). Expression of a plant‐derived peptide harboring water‐cleaning and antimicrobial activities. Biotechnology and Bioengineering, 81(1), 13-20.

Taiz, L., & Zeiger, E. (1991). Ethylene and abscisic acid. Plant Physiology. The Benjamin/Cummings Publishing Company, Redwood City, CA, 473-489.

Thorpe, T. A. (1982). Physiological and biochemical aspects of organogenesis in vitro. In Plant tissue culture 1982: proceedings, 5th International Congress of Plant Tissue and Cell Culture held at Tokyo and Lake Yamanake, Japan, July 11-16, 1982/edited by Akio Fujiwara. Tokyo: Japanese Association for Plant Tissue Culture,[1982?].

Ueda, Y., Uehara, N., Sasaki, H., Kobayashi, K., &Yamakawa, T. (2013). Impacts of acute ozone stress on superoxide dismutase (SOD) expression and reactive oxygen species (ROS) formation in rice leaves. Plant Physiology and Biochemistry, 70C,396-402.

Wojtania, A., & Skrzypek, E. (2014). Effects of cytokinins on antioxidant enzymes in in vitro grown shoots of Pelargonium hortorum LH Bayley. Acta agrobotanica, 67(4).

Zhang, J., Lin, M., Chen, H., & Chen, X. (2017). Agrobacterium tumefaciens-mediated transformation of drumstick (Moringa oleifera Lam.). Biotechnology and Biotechnological Equipment, 31(6), 1126–1131.



1. 04. 2019



Agronomy section

How to Cite

EL-NAGISH, A., HASSANEIN, A., SALEM, J., & FAHEED, F. (2019). Some important aspects in Moringa oleifera Lam. micropropagation. Acta Agriculturae Slovenica, 113(1), 13–27.

Similar Articles

1-10 of 461

You may also start an advanced similarity search for this article.