Some important aspects in Moringa oleifera Lam. micropropagation

Authors

  • Abdullah EL-NAGISH Botany and Microbiology Department, Faculty of Science, Sohag University, 82524 Sohag, Egypt
  • Ahmed HASSANEIN Botany and Microbiology Department, Faculty of Science, Sohag University, 82524 Sohag, Egypt
  • Jehan SALEM Botany and Microbiology Department, Faculty of Science, Sohag University, 82524 Sohag, Egypt
  • Fayza FAHEED Botany and Microbiology Department, Faculty of Science, Sohag University, 82524 Sohag, Egypt

DOI:

https://doi.org/10.14720/aas.2019.113.1.02

Keywords:

medium type, shoot multiplication, cytokinins, gene expression, isoenzymes, micropropagation, antioxidant enzymes, type of explant

Abstract

Type and source of explant as well as the type of cytokinin were important factors for successful moringa micropropagation. Explants obtained from in vitro grown plant materials were better than others obtained from soil growing seedlings. In addition, nodal segments were better than shoot cuttings in terms of number of shoots/ explants, frequency of shoot formation and number of nodes/shoot. While callus formation on the base of nodal segment on BAP containing media were higher than those of KIN, especially under the influence of high concentration as an aspect of vitrification, BAP was better than KIN in moringa multiplication. Low nutrient medium (half strength MS) supplemented with 0.5 mg l-1 IAA was essential for successful root formation. The suitable conditions for moringa micropropagation on full strength MS or SH may exert low stress and low need to raise the expression of SOD and POX. On the other side, stress due to over increase of chemical components of double MS medium or low nutrient content of half strength MS, B5 or WPM expressed the highest number and staining intensity of SOD and POX bands, vice versa was detected in case of CAT.

Author Biography

  • Abdullah EL-NAGISH, Botany and Microbiology Department, Faculty of Science, Sohag University, 82524 Sohag, Egypt

    Assistant lecturer at Botany and Microbiology Department, Faculty of Science, Sohag University, Sohag, Egypt


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Published

1. 04. 2019

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Agronomy section

How to Cite

EL-NAGISH, A., HASSANEIN, A., SALEM, J., & FAHEED, F. (2019). Some important aspects in Moringa oleifera Lam. micropropagation. Acta Agriculturae Slovenica, 113(1), 13–27. https://doi.org/10.14720/aas.2019.113.1.02

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