Comparative effects of cantharidin and endothall on gene expression and activity of antioxidant enzymes in Cichorium intybus L.

Authors

  • Yalda GHOTBZADEH KERMANI Science and Research University Of Tehran,Iran.
  • Ramazan Ali KHAVARI NEJAD Science and Research University Of Tehran,Iran.
  • Homa MAHMOODZADEH Mashhad branch, Islamic Azad University , Mashhad , Iran.
  • Khadijeh SHAHROKH ABADI Mashhad branch, Islamic Azad University , Mashhad , Iran.

DOI:

https://doi.org/10.14720/aas.2020.116.1.1232

Keywords:

cantharidin, catalase, Cichorium intybus L., endothall, glutathione reroxidase

Abstract

Cantharidin and its analog, endothall, are known to have phytotoxic effects and their mechanism of action involves the inhibition of phosphatases. Enzymes and antioxidant compounds act as barriers against phytotoxic compounds. Catalase and peroxidase are among the most important antioxidant enzymes. Cichorium intybus L. has traditionally been used for its medicinal properties and contains various phytochemical and enzymatic compounds. The present study aimed to investigate the comparative effects of cantharidin and endothall with concentration of 2.5, 5.5 and 10 µg ml-1 on the changes in the gene expression of catalase and glutathione peroxidase. Furthermore, we assess the activities of these enzymes in the shoots and roots of Cichorium intybus L.. According to the findings, the expression of catalase and glutathione peroxidase increased in the samples treated with cantharidin more than endothall compared to the controls in both shoot (the most significant is in cantharidin with 2.5 µg ml-1 concentration) and root samples (the most significant is in cantharidin with 5.5 µg ml-1  concentration). In addition, the activity of catalase and concentrations of cantharidin (2.5 µg ml-1) in shoot samples led to the more intense stimulation of catalase and glutathione peroxidase compared to root samples. We suggest that cantharidin and endothall have negative effect on expression and absorption of antioxidant enzymes.

Author Biographies

  • Yalda GHOTBZADEH KERMANI, Science and Research University Of Tehran,Iran.

    Department of Botany, College of Basic Science and Research Branch, Islamic Azad University, Tehran, Iran

  • Ramazan Ali KHAVARI NEJAD, Science and Research University Of Tehran,Iran.
    Department of  Botany , College of Basic Science and Research Branch , Islamic Azad University , Tehran , Iran.
  • Homa MAHMOODZADEH, Mashhad branch, Islamic Azad University , Mashhad , Iran.

    Department of  Biology, Mashhad branch,  Islamic Azad University , Mashhad , Iran.

  • Khadijeh SHAHROKH ABADI, Mashhad branch, Islamic Azad University , Mashhad , Iran.

    Department of  Biology, Mashhad branch,  Islamic Azad University , Mashhad , Iran.

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Published

25. 09. 2020

Issue

Section

Agronomy section

How to Cite

GHOTBZADEH KERMANI, Y., KHAVARI NEJAD, R. A., MAHMOODZADEH, H., & SHAHROKH ABADI, K. (2020). Comparative effects of cantharidin and endothall on gene expression and activity of antioxidant enzymes in Cichorium intybus L. Acta Agriculturae Slovenica, 116(1), 189–197. https://doi.org/10.14720/aas.2020.116.1.1232

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