Genetic studies of soybean [Glycine max (L.) Merr.] response to seed storage stress factors

Authors

  • Gbemisola Oluseun OKUNLOLA Federal university of Agriculture, Abeokuta, ogun State, Nigeria
  • Emmanuel IDEHEN Federal University of Agriculture, PMB 5320, Abeokuta, Nigeria
  • Johnson Adedayo ADETUMBI Institute of Agricultural Research and Training, Obafemi Awolowo University P.M.B. 5029, Moor Plantation, Ibadan, Nigeria
  • Christopher ALAKE Federal University of Agriculture, PMB 5320, Abeokuta, Nigeria
  • Solomon Tayo AKINYOSOYE Institute of Agricultural Research and Training, Obafemi Awolowo University P.M.B. 5029, Moor Plantation, Ibadan, Nigeria
  • Oluwafemi Daniel AMUSA Department of Cell Biology and Genetics, University of Lagos, Nigeria

DOI:

https://doi.org/10.14720/aas.2020.116.1.1181

Keywords:

soybean, seed accelerated aging, principal components analysis (PCA), dendrogram, SSR markers, tolerance

Abstract

Soybean [Glycine max (L.) Merr.] responded differently to storage stress factors. This research work aimed at assessing genetic potentials of fifteen soybean varieties to storage stress via accelerated aging technique and grouping them based on their levels of tolerance to storage stress using simple sequence repeat (SSR). Tolerance of the seed of 15 soybean varieties to storage stress was assessed by subjecting them to accelerated aging for 0, 6, 12, and 24 hours at 42 °C temperature and 100 % relative humidity (RH), after which their quality was assessed to determine their tolerance to storage stress. Same varieties were also stored for 6 months under ambient environment at 65 ± 5 % R.H and 25 ± 2 °C and they were genotyped with 19 simple sequence repeat (SSR) markers. The varieties were grouped on the basis of their levels of tolerance to storage stress. The principal components analysis (PCA) showed that germination rate index (GRI) and germination index (GI) were the major indices responsible for the significant variation in the seedling vigor characters. TGX1835-10E and TGX1448-2E were identified as varieties with good storage ability and therefore recommended for storage improvement in soybean breeding programs. Six SSR markers (Satt 565, Satt 175, Satt 281, Satt 600, Satt 160 and Satt 281) were identified as candidate markers for detection of alleles for tolerance to storage stress.

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Published

25. 09. 2020

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Section

Agronomy section

How to Cite

OKUNLOLA, G. O., IDEHEN, E., ADETUMBI, J. A., ALAKE, C., AKINYOSOYE, S. T., & AMUSA, O. D. (2020). Genetic studies of soybean [Glycine max (L.) Merr.] response to seed storage stress factors. Acta Agriculturae Slovenica, 116(1), 23–34. https://doi.org/10.14720/aas.2020.116.1.1181

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