Impact of different fermentation characteristics on the production of mycelial biomass, extra-cellular polysaccharides, intra-cellular polysaccharides, and on the antioxidant activities of Cordyceps militaris (L.) Fr. (strains AG-1, PSJ-1)

Authors

  • Dang Ngoc HUNG National Pingtung University of Science and Technology, Department of Tropical Agriculture and International Cooperation, Pingtung, 91201, Taiwan
  • Li Chun WANG National Pingtung University of Science and Technology, Department of Plant Industry, Pingtung, 91201, Taiwan
  • Liang Horng LAY National Pingtung University of Science and Technology, Department of Plant Industry, Pingtung, 91201, Taiwan
  • Vu Thi PHUONG Thai Nguyen University of Sciences, Thai Nguyen, 3Faculty of Tourism, 24000, Vietnam.

DOI:

https://doi.org/10.14720/aas.2020.116.2.1104

Keywords:

antioxidant activities, Cordyceps militaris, extra-cellular polysaccharides and intra-cellular polysaccharides, oven drying, pH

Abstract

The mycelial biomass, antioxidant activity and production of extra- and intra-cellular polysaccharides production [EPS, IPS] of Cordyceps militaris strains AG-1, PSJ-1 were evaluated under different submerged liquid culture (SLC) conditions. At 24 0C mycelial biomass and polysaccharide production of AG-1, PSJ-1 was optimal using PVC media and static culture conditions; (AG-1: 21.85 ± 1.00; PSJ-1: 18.20 ± 1.84 g l-1 ), and oven drying at 40 oC (AG-1: 25.95 ± 0.84, PSJ-1: 23.55 ± 0.69 mg g-1) compared with hot water extraction (AG-1: 7.07 ± 0.15, PSJ-1: 7.39 ± 0.61 mg g-1). Maximum biomass, EPS and IPS production were observed when the initial pH was 6.7: AG-1: 12.92 ± 0.33, 209.70 ± 1.56, 32.62 ± 0.87; PSJ-1: 9.03 ± 0.24 g l-1 , 198.16 ± 0.85 mg g-1, 30.63 ± 1.96 mg g-1, respectively. The use 3.5 % coconut oil improved biomass, EPS, IPS production, which were 8.27 ± 0.09, 8.01 ± 0.01 g l-1 ; 1208.00 ± 8.60, 1110.40 ± 7.20 mg g-1; 32.43 ± 0.49, 29.74 ± 0.44, for AG-1 and PSJ-1, respectively. Both culture condition and oven drying methods had significant effects on H2O2 and radical scavenging activity, ABTS.+ radical activity, lipid peroxidation, and also had effects on total flavonoid and, total phenolic contents. The use of crude submerged liquid culture and oven drying on strains AG-1, PSJ-1 led to extracts with potent antioxidant activity, suggesting the therapeutic use of polysaccharides from strains AG-1, PSJ-1. 

Author Biographies

  • Dang Ngoc HUNG, National Pingtung University of Science and Technology, Department of Tropical Agriculture and International Cooperation, Pingtung, 91201, Taiwan
    Department of Tropical Agriculture and International Cooperation
  • Li Chun WANG, National Pingtung University of Science and Technology, Department of Plant Industry, Pingtung, 91201, Taiwan
    Department of Plant Industry
  • Liang Horng LAY, National Pingtung University of Science and Technology, Department of Plant Industry, Pingtung, 91201, Taiwan
    Department of Plant Industry
  • Vu Thi PHUONG, Thai Nguyen University of Sciences, Thai Nguyen, 3Faculty of Tourism, 24000, Vietnam.
    Faculty of Tourism

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Published

23. 12. 2020

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Section

Agronomy section

How to Cite

HUNG, D. N., WANG, L. C., LAY, L. H., & PHUONG, V. T. (2020). Impact of different fermentation characteristics on the production of mycelial biomass, extra-cellular polysaccharides, intra-cellular polysaccharides, and on the antioxidant activities of Cordyceps militaris (L.) Fr. (strains AG-1, PSJ-1). Acta Agriculturae Slovenica, 116(2), 337–350. https://doi.org/10.14720/aas.2020.116.2.1104