Symbiotic and physiological indicators of soybean inoculated of Bradyrhizobium japonicum single-strain in 7 days before sowing

Authors

  • Nadiya VOROBEY Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine, Ukraine
  • Kateryna KUKOL Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine, Ukraine
  • Petro PUKHTAIEVYCH The Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine, Ukraine
  • Tetyana KOTS Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine, Institute of the Ukrainian Language, National Academy of Sciences of Ukraine, Ukraine

DOI:

https://doi.org/10.14720/aas.2022.118.2.1867

Keywords:

rhizobia, Bradyrhizobium japonicum, bacterial preparations, pre-sowing inoculation, nitrogen fixing activity, soybean

Abstract

Results of investigation of soybean of the Almaz variety in inoculation with preparations based on nodule bacteria Bradyrhizobium japonicum (Kirchner, 1896), Jordan, 1982 B78, B157, D37, D87 are presented. Different periods of the soybean seeds inoculation were used - on the sowing day (control) and in 7 days before sowing (experimental variants). The differences between control and experimental plants in the formation and functioning of the symbiotic apparatus and its functional activity, depending on the period between from seed inoculation to sowing were analysed. It was determined that the number of root nodules in the control plants was higher. The mass of nodules at the stage of 3 true leaves exceeded the control by 1.5–2.0 times in plants inoculated in 7 days before sowing, and the intensity of nitrogen fixation by 1.7–6.6 times. At the budding-beginning of flowering stage, the mass and intensity of N2 fixation by the nodules of control plants increased. As a result, the difference between the nitrogen fixing activity of control and experimental plants decreased significantly. Stimulating effect on aboveground mass of Bradyrhizobium japonicum strains with increased nitrogen fixing activity was noted. Optimal conditions for the formation and functioning of bean-rhizobial symbiosis were provided at the use of both of these terms of soybean inoculation. This reveals the possibility of effective application of early inoculation of soybean seeds with preparations based on nodule bacteria Bradyrhizobium japonicum active strains.

Author Biographies

  • Nadiya VOROBEY, Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine, Ukraine
    Senior researcher of the department of symbiotic nitrogen fixationCandidate of Biological Sciences (Ph. D.)
  • Kateryna KUKOL, Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine, Ukraine
    Research Officer of the department of symbiotic nitrogen fixationCandidate of Biological Sciences (Ph. D.)
  • Petro PUKHTAIEVYCH, The Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine, Ukraine
    Research Officer of the department of symbiotic nitrogen fixationCandidate of Biological Sciences (Ph. D.)
  • Tetyana KOTS, Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine, Institute of the Ukrainian Language, National Academy of Sciences of Ukraine, Ukraine
    Doctor of Philological Sciences

References

Agritema. Catalog of products. (2017). Retrieved from https://agritema.com/wp-content/uploads/2017/09/AGRITEMA-Catalog_UA.pdf

Anghinoni, F. B. G., Braccini, A. L., Scapim, C. A., Anghinoni, G., Ferri, G. C., Suzukawa, A. K. & Tonin, T. A. (2017). Pre-inoculation with Bradyrhizobium spp. in industrially treated soybean seeds. Agricultural Science, 8(7), 582–590. https://doi.org/10.4236/as.2017.87044

Berbenets, О. V. (2019). World-wide production of soya as an inexhaustible source of vegetable proteins and Ukraine’s place in the global trading market. Agrosvit, 10, 41–45. https://www.doi.org/10.32702/2306-6792.2019.10.41

Deaker, R., Hartley, E., & Gemell, G. (2012). Conditions affecting shelf-life of inoculated legume seed. Agriculture, 2(1), 38–51. https://doi.org/10.3390/agriculture2010038

Gemell, L. G., Hartley, E. J., & Herridge, D. F. (2005). Point-of-sale evaluation of preinoculated and custom-inoculated pasture legume seed. Australian Journal of Experimental Agriculture, 45(3), 161–169. https://doi.org/10.1071/EA03151

Grishechkin, V. V. & Golovina, E. V. (2014). Use of new organic film-former (PPO) for conservation of viability of rhizobia at inoculation of seeds of soya and their influence on formation of nodules and productivity. Legumes and Groat Crops, 1(9), 41–44.

Grodzinsky, A. M. & Grodzinsky, D. M. (1964). Short reference book on plant physiology, Kyiv: Nauk. Dumka.

Hardy, R. W. F., Holsten, R. D., Jackson, E. K. & Burns, R. C. (1968). The acetylene – ethylene assay for N2 fixation: laboratory and field evaluation. Plant Physiology, 42(8), 1185–1207. https://doi.org/10.1104/pp.43.8.1185

Kirizii, D. A., Vorobei, N. A. & Kots, S. Ya. (2007). Relationships between nitrogen fixation and photosynthesis as the main components of the productivity in alfalfa. Russian Journal of Plant Physiology. 54(5), 666–671. https://doi.org/10.1134/S1021443707050032

Kokorina, A. L. & Kozhemyakov, A. P. (2010). The Rhizobium-legume symbiosis and the use of microbiological preparations of complex action are an important reserve for increasing the productivity of arable land. Saint Petersburg: All-Russia Research Institute for Agricultural Microbiology.

Kots, S & Mamenko, P. (2015). Soybean inoculation and incrustation: a review of application technology and market of prerarats. Proposition. Special issue. Modern agrotechnologies for the use of biological products and growth regulators, 24-28.

Kots, S. Ya. (2011). Сurrent state of biological nitrogen fixation studies. Physiology and Biochemistry of Cultivated Plants, 43(3), 212–225.

Kots, S. Ya., Vorobey, N. A., Kyrychenko, O. V., Melnykova, N. N., Mykhalkiv, L. M. & Pukhtaievych, P. P. (2016). Microbiological Preparations for Agriculture, Kyiv: Logos.

Kramarev, S. M. & Artemenko, S. F. (2016). The productivity of maize in crop short rotations with soybeans in the conditions of northern Steppe of Ukraine. News of Dnipropetrovsk State Agrarian and Economic University, 42(4), 68–71.

Laktionov, Yu. V., Kosulnikov, Y. V., Dudnicova, D. V., Yahno, V. V. & Kojemyakov, A. P. (2019). Pre-sowing protection of inoculated soybean Glycine max (L.) Merr. seeds by water-soluble polymer compositions and their solid-phase modification. Agricultural Biology, 54(5), 1052–1059 https://doi.org/10.15389/agrobiology.2019.5.1052eng

Laktionov, Yu. V., Kosulnikov, Yu. V. & Dudnikova, D. V. (2018). The effect of water-soluble polymers on the survival of nodule lupine bacteria (Rhizobium lupini). Grain Economy of Russia, 3(57), 22–26. https://doi.org/10.31367/2079-8725-2018-57-3-22-26

Martyniuk, S., Oron, J., Martyniuk, M. & Wozniakowska, A. (2002). Effects of interactions between chemical seed dressings and Bradyrhizobium japonicum on soybean seeds. Archives of Agronomy and Soil Science, 48(4), 305–310. https://doi.org/10.1080/03650340214202

Melnykova, N. M. (2019). Effect of rhizobial exopolysaccharides on soybean seed germination and nodule development in the soybean-rhizobia symbiosis. Plant Physiology and Genetics, 51(5), 436–446. https://doi.org/10.15407/frg2019.05.436

Morgun, V. V. & Kots, S. Ya. (2008). Symbiotic nitrogen fixation and its significance in nitrogen plant nutrition: research status and prospects. Physiology and Biochemistry of Cultivated Plants, 40(3), 187–205.

Netrusov, A. I., Yegorova, M. A., Zakharchuk, L. M., Kolotilova, N. N., Kotova, I. B., Semenova, E. V., … Judina, T. G. (2005). Workshop on Microbiology. Moscow: Akademiya Publ.

Patyka, V. P. & Petrychenko, V. F. (2004). Microbial nitrogen fixation in modern fodder production. Feeds and Feed Production, 53, 3–6.

Pavlyshche, A. V., Kiriziy, D. A. & Kots, S. Ya. (2017). The reaction of symbiotic soybean systems to the action of fungicides under various treatment. Plant Physiology and Genetics, 49(3), 237‒247. https://doi.org/10.15407/frg2017.03.237

Slobodyanyuk, O. (2017). The nodules for soybean. Rizoline is a new answer for an old question. Agri Business Today, 347(5), 64–65.

Spaink, H., Kondorosi, A & Hooykaas, P. (2002). The Rhizobiaceae Molecular Biology of Model Plant – Assosiated Bacteria. Translated in rus. by Tikhonovich, I. A. & Provorov, N. A. St. Petersburg: Biont.

Zilli, J. E., Campo, R. J. & Hungria, M. (2010). Effectiveness of Bradyrhizobium inoculation at pre-sowing of soybean. Pesquisa Agropecuaria Brasileira, 45(3), 335–337. https://doi.org/10.1590/S0100-204X2010000300015

Downloads

Published

8. 07. 2022

Issue

Section

Original Scientific Article

How to Cite

VOROBEY, N., KUKOL, K., PUKHTAIEVYCH, P., & KOTS, T. (2022). Symbiotic and physiological indicators of soybean inoculated of Bradyrhizobium japonicum single-strain in 7 days before sowing. Acta Agriculturae Slovenica, 118(2), 1–11. https://doi.org/10.14720/aas.2022.118.2.1867

Similar Articles

1-10 of 618

You may also start an advanced similarity search for this article.