Genotypic variation in response to drought stress is associated with biochemical and transcriptional regulation of ureides metabolism in common bean (Phaseolus vulgaris L.)

Authors

  • Motlalepula PHOLO-TAIT Botswana University of Agriculture and Natural Resources, Botswana
  • Thuto KGETSE Botswana International University of Science and Technology, Botswana
  • Gaone Nthabeleng TSHEKO Botswana International University Science and Technology, Botswana
  • Olerato Tshotlhe THEDI Boswana International University of Science and Technology, Botswana
  • Katso LETHOLA Department of Agricultural Research, Botswana
  • Ebenezer Oteng MOTLAMME Department of Agricultural Research Ministry of Agricultural Development and Food Security, Botswana
  • Moagisi Innocent ITHUTENG Department of Agricultural Research Ministry of Agricultural Development and Food Security, Botswana
  • Samodimo NGWAKO Botswana University of Agriculture and Natural Resources, Botswana

DOI:

https://doi.org/10.14720/aas.2022.118.2.2541

Keywords:

common bean, drought stress, ureides, allantonaise, allantoin, allantoate

Abstract

Ureidic legumes such as common bean (Phaseoulus valgaris L.) plants export nitrogen from the nodules to shoots and leaves as ureides during symbiotic biological nitrogen fixation. Common bean gene encoding allantoinase (allantoin amidohydrolase, EC 3.5.2.5), is a key enzyme that catalyses the hydrolysis of allantoin to allantoic acid. It plays a role in ureide generation for export and ureide catabolism to generate a nitrogen source in sinks tissues. As such, one of the adaptive mechanisms of plants to drought stress, is associated with ureides accumulation. To identify genetic variation of common bean in response to drought stress, changes in the expression of ALLANTONAISE (PvALN) gene and ureides content were examined in the leaf tissues of the three common bean genotypes (CAL96, DAB514 and DAB541) and one tepary bean genotype (Phaseolus acutifolius A.Gray). Amongst all the genotypes, the suggested drought susceptibility in DAB514 common bean genotype, was probably attributed to a repressed PvALN expression rate which were corroborated by an impaired ureides levels, and reduced plant growth. On contrary, drought stress induced an upregulated relative expression of PvALN coupled with an increase in allantoin and allantoate in DAB541 common bean genotype. In addition, the sustained plant growth in CAL96 was probably attributed to a steady amount of allantoin synthesized under drought stress. Taken together, DAB541 and CAL96 common bean genotypes are the promising genotypes with an induced upregulated transcriptional control of catabolism and/or biosynthesis of ureides, hence potential genotypes for selection and introduction under Botswana semi-arid conditions.

Author Biographies

  • Motlalepula PHOLO-TAIT, Botswana University of Agriculture and Natural Resources, Botswana

    Plant Biotechnology and Molecular Biology

    Manager-Research Development and Outreach

    Botswana  University of Agriculture and Natural Resources

     

  • Thuto KGETSE, Botswana International University of Science and Technology, Botswana

    BSc student

    Department of Chemistry & Forensic Sciences

    Botswana International University Science and Technology

  • Gaone Nthabeleng TSHEKO, Botswana International University Science and Technology, Botswana

    BSc student

    Department of Chemistry & Forensic Sciences

    Botswana International University Science and Technology

  • Olerato Tshotlhe THEDI, Boswana International University of Science and Technology, Botswana

    BSc student

    Department of Chemistry & Forensic Sciences

    Botswana International University Science and Technology

  • Katso LETHOLA, Department of Agricultural Research, Botswana

    Crop Researcher

    Department of Agricultural Research

  • Ebenezer Oteng MOTLAMME, Department of Agricultural Research Ministry of Agricultural Development and Food Security, Botswana

    Research Intern

    Deaprtment of Agricultural Research

  • Moagisi Innocent ITHUTENG, Department of Agricultural Research Ministry of Agricultural Development and Food Security, Botswana

    Biometry and Statistics

    Research officer

    Department of Agriculture and Natural Resources

    Ministry of Agricultural Development and Food Security

  • Samodimo NGWAKO, Botswana University of Agriculture and Natural Resources, Botswana

    Professor-Agronomy and Bbreeding

    Dean-Research and Graduate Studies

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Published

8. 07. 2022

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Original Scientific Article

How to Cite

PHOLO-TAIT, M., KGETSE, T., TSHEKO, G. N., THEDI, O. T., LETHOLA, K., MOTLAMME, E. O., ITHUTENG, M. I., & NGWAKO, S. (2022). Genotypic variation in response to drought stress is associated with biochemical and transcriptional regulation of ureides metabolism in common bean (Phaseolus vulgaris L.). Acta Agriculturae Slovenica, 118(2), 1–9. https://doi.org/10.14720/aas.2022.118.2.2541

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