Clonal propagation of Tetragonolobus palaestinus Bioss: A Jordanian medical plant

Authors

  • Mawadda MHERAT Faculty of Agricultural technology, Al Balqa Applied University, Salt, Jordan
  • Mohammad SHATNAWI Faculty of Agricultural technology, Al Balqa Applied University, Salt, Jordan
  • Rida SHIBLI Al Ahliyya Amman University (and The University of Jordan), Faculty of Agricultural technology, Amman, Jordan
  • Tamara QUDAH Hamdi Mango Centre for Scientific Research, University of Jordan, Jordan
  • Saida ABU MALLOH Hamdi Mango Centre for Scientific Research, University of Jordan, Jordan
  • Tamadour AL-QUDAH Department of Nutrition and Food Technology, Faculty of Agriculture, Mutah University, Karak, Jordan

DOI:

https://doi.org/10.14720/aas.2022.118.3.1208

Keywords:

acclimatization, in vitro, shoot multiplication, rooting

Abstract

Tetragonolobus palaestinus Bioss (Aljalaton) is one of the Jordanian medicinal plants that can be used to treat stomach pain and some infections. This study was done in order to establish optimal in vitro propagation method for T. palaestinus. Factors of in vitro shooting, rooting, and acclimatization of the in vitro Tetragonolobus palaestinus seedlings were studied using different growth regulators. For in vitro shooting, different cytokinins including benzylamino purine (BAP), kinetin, TDZ, and zeatin were used in increasing concentrations (0.0, 0.3, 0.6, 0.9, 1.2, 1.5, and 2.0 mg l-1). Using benzylamino purine (BAP produced a maximum of 2.0 shoots/explants on Murashige and Skoog (MS) medium supplemented with 0.3 mg l-1. Moreover, the effect of different concentrations of IBA (indole-3-butyric acid), IAA (indole-3-acetic acid), andnaphthalene acetic acid (NAA) was evaluated for in vitro rooting. The highest number of roots (4.06 roots/explant) was obtained on MS medium supplemented with 0.3 mg l-1 IBA. All of the plants (100 %) were grown normally after the acclimatization process. Based on these results simple protocol of T. palaestinus in vitro culture was optimized for the first time which can be utilized to do more studies on cell culture and production of active secondary metabolites.

References

Adugna, A. Y., Feyissa, T., & Tasew, F. S. (2020). Optimization of growth regulators on in vitro propagation of Moringa stenopetala from shoot explants. BMC Biotechnology, 20(1), 1-10. https://doi.org/10.1186/s12896-020-00651-w

Adwan, G., Abu-shanab, B., & Adwan, K. (2009). In vitro activity of certain drugs in combination with plant extracts against Staphylococcus aureus infections. African Journal of Biotechnology, 8(17), 4239-4241

Afifi, F. U., & Abu-Irmaileh, B. (2000). Herbal medicine in Jordan with special emphasis on less commonly used medicinal herbs. Journal of Ethnopharmacology, 72(1-2), 101-110. https://doi.org/10.1016/S0378-8741(00)00215-4

Ahmad, N., Faisal, M., Anis, M., & Aref, I. M. (2010). In vitro callus induction and plant regeneration from leaf explants of Ruta graveolens L.. South African Journal of Botany, 76(3), 597-600. https://doi.org/10.1016/j.sajb.2010.03.008

Ahmadi Hesar, A., Kaviani, B., Tarang, A. R., & Zanjani, S. B. (2011). Effect of different concentrations of kinetin on regeneration of ten weeks (Matthiola incana). Plant Omics Journal, 4(5), 236-38.

Ahmed MR, Anis M (2012) Role of TDZ in the quick regeneration of multiple shoots from nodal explant of Vitex trifolia L. an important medicinal plant. Applied Biochemistry and Biotecnology, 168, 957–966. https://doi.org/10.1007/s12010-012-9799-0

Al-Bakri, J. T., Al-Eisawi, D., Damhoureyeh, S., & Oran, S. (2011). GIS-based analysis of spatial distribution of medicinal and herbal plants in arid and semiarid zones in the Northwest of Jordan. Annals of Arid Zone, 50(2), 99-115.

Alenizi, A., Shibli, R. A., Tahtamouni, R. W., Al-Qudah, T. S., & Abu-Iramaileh, B. (2020). In vitro propagation and enhancement of quercetins and isorhamnetin production in wild Paronychia argentea L. Jordan Journal of Pharmaceutical Sciences, 13(1).

Al‐Karaki, G. N. (2000). Morphological and yield traits of wild legume (Tetragonolobus palaestinus Boiss) populations. Journal of Agronomy and Crop Science, 184(4), 267-270. https://doi.org/10.1046/j.1439-037x.2000.00393.x

Al-Mahmood, H. J., Shatnawi, M. A., Shibli, R. A., Makhadmeh, I. M., Abubaker, S. M., & Shadiadeh, A. N. (2012). Clonal propagation and medium-term conservation of Capparis spinosa: a medicinal plant. Journal of Medicinal Plants Research, 6(22), 3826-3836. https://doi.org/10.5897/JMPR11.547

Al-Qudah, T. S., Shibli, R. A., & Alali, F. Q. (2011). In vitro propagation and secondary metabolites production in wild germander (Teucrium polium L.). In Vitro Cellular & Developmental Biology-Plant, 47(4), 496-505. https://doi.org/10.1007/s11627-011-9352-9

Arafeh, R. M., Shibli, R. A., Al-Mahmoud, M., & Shatnawi, M. A. (2006). Callusing, cell suspension culture and secondary metabolites production in Persian oregano (Origanum vulgare L.) and Arabian oregano (O. syriacum L.). Jordan Journal of Agricultural Sciences, 2(3), 274-81.

Cenkci, S., Kargioglu, M., Dayan, S., & Konuk, M. (2008). In vitro propagation of an endangered plant species, Thermopsis turcica (Fabaceae). Biologia, 63(5), 652-657. https://doi.org/10.2478/s11756-008-0125-9

Dewir, Y. H., Murthy, H. N., Ammar, M. H., Alghamdi, S. S., Al-Suhaibani, N. A., Alsadon, A. A., & Paek, K. Y. (2016). In vitro rooting of leguminous plants: Difficulties, alternatives, and strategies for improvement. Horticulture, Environment, and Biotechnology, 57(4), 311-322. https://doi.org/10.1007/s13580-016-0060-6

Ebrahim, N., Shibli, R., Makhadmeh, I., Shatnawi, M., & Abu-Ein, A. (2007). In vitro propagation and in vivo acclimatization of three coffee cultivars (Coffea arabica L.) from Yemen. World Applied Sciences Journal, 2(2), 142-150.

García-Fortea, E., Lluch-Ruiz, A., Pineda-Chaza, B. J., García-Pérez, A., Bracho-Gil, J. P., Plazas, M., ... & Prohens, J. (2020). A highly efficient organogenesis protocol based on zeatin riboside for in vitro regeneration of eggplant. BMC plant Plant Biology, 20(1), 1-16. https://doi.org/10.1186/s12870-019-2215-y

George, E. F., Hall, M. A., & De Klerk, G. J. (2008). Micropropagation: uses and methods. In Plant propagation by tissue culture, (pp. 29-64). Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5005-3_2

Haygert-Lencina, K., Antônio-Bisognin, D., Kielse, P., & Pimentel, N. (2017). Rooting and acclimatization of Apuleia leiocarpa plantlets. Agrociencia, 51(8), 909-920.

Kumar S, Singh N (2010). Micropropagation of Prosopis cineraria (L.) Druce.: a multipurpose desert tree. Report and Opinion, 2, 44–47.

Lee, R. X., Hassan, Z., Subramaniam, S., & Chew, B. L. (2021). Adventitious root cultures of Clitoria ternatea L. and its potential as a memory enhancer alternative. Plant Biotechnology Reports, 15(2), 163-176. https://doi.org/10.1007/s11816-021-00664-7

Lijalem, T., & Feyissa, T. (2020). In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant. Journal of Genetic Engineering and Biotechnology, 18(1), 1-10. https://doi.org/10.1186/s43141-019-0017-0

Makhadmeh, I. M., & Shatnawi, M. A. (2008). In vitro propagation of threatened Pimelea spicata from mature plant material. Advances in Horticultural Science, 212-217.

Malik KA, Saxena PK (1992) Regeneration in Phaseolus vulgaris L.: high frequency induction of direct shoot formation in intact seedlings by N6- benzylaminopurine and thidiazuron. Planta, 186,384–389. https://doi.org/10.1007/BF00195319

Mineo, L. (1990). Plant tissue culture techniques. Tested studies for laboratory teaching, 11, 151-174.

Mony, S. A., Haque, M. S., Alam, M. M., Hasanuzzaman, M., & Nahar, K. (2010). Regeneration of blackgram (Vigna mungo L.) on changes of hormonal condition. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38(3), 140-145.

Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Naeem, M., Bhatti, I. R. A. M., Ahmad, R. H., & Ashraf, M. Y. (2004). Effect of some growth hormones (GA3, IAA and Kinetin) on the morphology and early or delayed initiation of bud of lentil (Lens culinaris Medik). Pakistan Journal of Botany, 36(4), 801-809.

Qrunfleh, I. M., Shatnawi, M. M., & Al-Ajlouni, Z. I. (2013). Effect of different concentrations of carbon source, salinity and gelling agent on in vitro growth of fig (Ficus carica L.). African Journal of Biotechnology, 12 (90), 936-940.

Quisen, R. C., Raizer, M. D. M., & Iriarte-Martel, J. H. (2013). Acclimatization of micropropagated plantlets of Heliconia sexy Pink. Embrapa Amazônia Ocidental-Artigo em Periódico Indexado (ALICE), 56, Supl., 1-5. https://doi.org/10.4322/rca.2013.073

Rout, G. R., Samantaray, S., & Das, P. (2000). In vitro rooting of Psoralea corylifolia Linn: Peroxidase activity as a marker. Plant Growth Regulation, 30(3), 215-219. https://doi.org/10.1023/A:1006336819887

Shaheen, A., Ali, M., Ahmad, N., Hassan Dewir, Y., El-Hendawy, S., Gawad, A. E., & Ahmed, M. (2020). Micropropagation of licorice (Glycyrrhiza glabra L.) by using intermediate nodal explants. Chilean Journal of Agricultural Research, 80(3), 326-333. https://doi.org/10.4067/S0718-58392020000300326

Sharma, R., & Shahzad, A. (2008). Thidiazuran (TDZ) induced regeneration from cotyledonary node explant of Abelmoschus moschatus Medik. L., (A valuable medicinal plant).World Journal of Agricultural Sciences, 4(4), 449-452.

Shatnawi, M. A. (2006). Micropropagation and germplasm storage of Prunus amygdalus by the vitrification method. Jordan Journal of Agricultural Sciences, 2(3), 222-233.

Shatnawi, M. A. (2013). Multiplication and cryopreservation of yarrow (Achillea millefolium L., Astraceae). Journal of Agriculture Science and Technology. 15, 163-173.

Shatnawi, M. A., Shibli, R. A., Abu-Romman, S. M., Al-Mazra, M. S., Al Ajlouni, Z. I., Shatanawi, A., & Odeh, W. H. (2011). Clonal propagation and cryogenic storage of the medicinal plant Stevia rebaudiana. Spanish Journal of Agricultural Research, 9(1), 213-220. https://doi.org/10.5424/sjar/20110901-021-10

Shibli, R. A., Sharaf, S. A., Kasrawi, M. A., & Al-Qudah, T. S. (2018). In vitro multiplication of the white wormwood, Artemisia herba-alba Asso. Jordan Journal of Biological Sciences, 11(3).

Shibli, R. A., Shatnawi, M. A., & Swaidat, I. Q. (2003). Growth, osmotic adjustment, and nutrient acquisition of bitter almond under induced sodium chloride salinity in vitro. Communications in Soil Science and Plant Analysis, 34(13-14), 1969-1979. https://doi.org/10.1081/CSS-120023231

Silva, D., Imakawa, A. M., Bruno, F. M. S., de Souza Costa, S., & Sampaio, P. D. T. B. (2018). In vitro propagation and seedling acclimatization of Caesalpinia ferrea Mart., a valuable medicinal plant in the Amazon (Fabaceae). Boletin do Museu Paraense Emílio Goeldi-Ciências Naturais, 13(1), 57-65. https://doi.org/10.46357/bcnaturais.v13i1.368

Singh, R. K., Anandhan, S., García-Pérez, L. M., Ruiz-May, E., Pérez, E. N., & Quiroz-Figueroa, F. R. (2019). An efficient protocol for in vitro propagation of the wild legume Cicer microphyllum Benth., a crop wild relative of chickpea (Cicer arietinum L.). In Vitro Cellular & Developmental Biology-Plant, 55(1), 9-14. https://doi.org/10.1007/s11627-018-09958-y

Singh, V., Chauhan, N. S., Singh, M., Idris, A., Madanala, R., Pande, V., & Mohanty, C. S. (2014). Establishment of an efficient and rapid method of multiple shoot regeneration and a comparative phenolics profile in in vitro and greenhouse-grown plants of Psophocarpus tetragonolobus (L.) DC. Plant Signaling & Behavior, 9(10), e970443. https://doi.org/10.4161/15592316.2014.970443

Thảo, N. T., Thảo, N. T. P., Hassan, F., & Jacobsen, H. J. (2013). In vitro propagation of common bean (Phaseolus vulgaris L.). Journal of Science and Development, 11(6), 868-876.

Tiwari AK, Singh D, Tripathi S, Mishra N, Singh RB (2010) Effect of BAP and kinetin on shoot initiation of Trichosanthes dioica Roxb: An important medicinal plant. Journal of Medicinal Plants, 2, 59–61. https://doi.org/10.5958/j.0975-4261.2.1.008

Ugandhar, T., Venkateshwarlu, M., Sammailah, D., & Reddy, J. M. K. (2012). Rapid in vitro micro propagation of chick pea (Cicer arietinum L.) from shoot tip and cotyledonary node explants. Journal Biotechnol Biomater, 2(6), 1-6.

Veltcheva, M. R., & Lilova Svetleva, D. (2005). In vitro regeneration of Phaseolus vulgaris L. via organogenesis from petiole explants. Journal of Central European Agriculture, 6(1), 53-58.

Vidoz, M. L., Quesenberry, K. H., Real, D., & Gallo, M. (2012). Plant regeneration of Lotononis bainesii Baker (Fabaceae) through cotyledon and leaf culture. African Journal of Biotechnology, 11(41), 9724-9731. https://doi.org/10.5897/AJB11.2680

Vikram, G., Madhusudhan, K., Srikanth, K., Laxminarasu, M., & Swamy, N. R. (2012). Zeatin induced direct multiple shoots development and plant regeneration from cotyledon explants of cultivated tomato (Solanum lycopersicum L.). Australian Journal of Crop Science, 6(1), 31-35.

Downloads

Published

20. 10. 2022

Issue

Section

Original Scientific Article

How to Cite

MHERAT, M., SHATNAWI, M., SHIBLI, R., QUDAH, T., ABU MALLOH, S., & AL-QUDAH, T. (2022). Clonal propagation of Tetragonolobus palaestinus Bioss: A Jordanian medical plant. Acta Agriculturae Slovenica, 118(3), 1–9. https://doi.org/10.14720/aas.2022.118.3.1208

Similar Articles

1-10 of 123

You may also start an advanced similarity search for this article.