Molecular diversity of rice (Oryza sativa L.) genotypes in Malaysia based on SSR markers

Authors

  • Mohammad ANISUZZAMAN Universiti Putra Malaysia, Malaysia
  • Mohammad Rafiqul ISLAM Bangladesh Rice Research Institute, Bangladesh
  • Hasina KHATUN Bangladesh Rice Research Institute, Bangladesh
  • Mohammad Amdadul HAQUE Bangladesh Agricultural Research Institute, Bangladesh
  • Mahammad Shariful ISLAM Bangladesh Agricultural Research Institute, Bangladesh
  • Mohammad Shamim AHSAN Bangladesh Agricultural Research Institute, Bangladesh

DOI:

https://doi.org/10.14720/aas.2022.118.4.2500

Keywords:

molecular diversity, SSR markers, polymorphic information content, rice

Abstract

Rice crop improvement is determined by the degree of genetic variability and the heritability of favorable genes. A total of twenty-five SSR markers were used to measure the level of polymorphism and genetic variation among the 65 rice genotypes. Twenty-one of the twenty-five SSRs were discovered to be polymorphic, whereas the rest were determined to be monomorphic. A total of 91 alleles were found in 21 SSR markers, with an average of 4.00 alleles which ranged from 3 (RM335, RM551, RM538 RM190, RM242 and RM270) to 7 (RM263). The average PIC value was 0.62 ranging from 0.28 (RM 270) to 0.76 (RM 481). The rice genotypes were divided into nine primary clusters by a dendrogram based on NTSYS software’s UPGMA analysis. The cluster analysis revealed that these genotypes were divided into nine clusters where cluster IB-1a has the most genotypes (31) followed by cluster IB-1b (24).The genotype BR24 and Utri as well as Pukhi and WANGI PUTEH had the highest dissimilarity coefficient values indicating genotype diversity. These accessions have a lot of genetic diversity among the constituents; thus, they could be used directly in a hybridization program to improve yield-related parameters.

Author Biographies

  • Mohammad ANISUZZAMAN, Universiti Putra Malaysia, Malaysia
    Faculty of Agriculture and Senior Scientific Officer
  • Mohammad Rafiqul ISLAM, Bangladesh Rice Research Institute, Bangladesh
    Plant Breeding Division and Senior Scientific Officer
  • Hasina KHATUN, Bangladesh Rice Research Institute, Bangladesh
    Plant Breeding Division and Senior Scientific Officer
  • Mohammad Amdadul HAQUE, Bangladesh Agricultural Research Institute, Bangladesh
    Pomology division and Senior Scientific Officer
  • Mahammad Shariful ISLAM, Bangladesh Agricultural Research Institute, Bangladesh
    On-Farm Research Division and Senior Scientific Officer
  • Mohammad Shamim AHSAN, Bangladesh Agricultural Research Institute, Bangladesh
    Plant Physiology Division and Senior Scientific Officer

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Published

30. 12. 2022

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Original Scientific Article

How to Cite

ANISUZZAMAN, M., ISLAM, M. R., KHATUN, H., HAQUE, M. A., ISLAM, M. S., & AHSAN, M. S. (2022). Molecular diversity of rice (Oryza sativa L.) genotypes in Malaysia based on SSR markers. Acta Agriculturae Slovenica, 118(4), 1–13. https://doi.org/10.14720/aas.2022.118.4.2500

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