Antioxidant response of Impatiens walleriana L. to drought


  • Anamarija MATIJEVIĆ Faculty of Agriculture and Food Sciences University of Sarajevo
  • Ajla ŠAKONJIĆ Faculty of Agriculture and Food Sciences University of Sarajevo
  • Senad MURTIĆ Department of Plant Physiology, Faculty of Agriculture and Food Sciences University of Sarajevo



defence system, free radicals, leaves, plant growth, stress


Stress caused by drought induces plant morphology, biochemistry, and physiology changes, leading to considerable reductions in plant growth and productivity. This study aimed to evaluate the antioxidant defence system of impatiens seedlings (Impatiens walleriana L.) under drought. The antioxidant response of impatiens to drought was evaluated using following parameters: the activity of catalase, guaiacol peroxidase, pyrogallol peroxidase and ascorbate peroxidase, total phenolic and flavonoids contents and total antioxidant capacity. The experiment was conducted during 2020 in a greenhouse under controlled conditions. Half of the impatiens seedlings (20 plants), after the acclimation period in the greenhouse, were exposed to drought for a period of five days, while the second half was not (20 plants were regularly watered). The results of the study showed that the exposure of impatiens seedlings to drought increased the activity of enzymatic components, total phenolics and flavonoids contents and total antioxidant capacity of leaves. Greater exposure of impatiens to drought (in the observed period) implied a higher plant enzymatic and non-enzymatic antioxidant defence system activity. These results confirm that impatiens has evolved both enzymatic and non-enzymatic antioxidant defence mechanisms to adapt and survive the short-term drought exposure.

Author Biographies

Anamarija MATIJEVIĆ, Faculty of Agriculture and Food Sciences University of Sarajevo

Department of Plant Physiology

Ajla ŠAKONJIĆ, Faculty of Agriculture and Food Sciences University of Sarajevo

Department of Plant Physiology

Senad MURTIĆ, Department of Plant Physiology, Faculty of Agriculture and Food Sciences University of Sarajevo

Department of Plant Physiology


Abedi, T., & Pakniyat, H. (2010). Antioxidant enzyme changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.). Czech Journal of Genetics and Plant Breeding, 46, 27–34.

Aebi, M. (1984). Catalase in vitro. Methods in Enzymology, 105, 121–126.

Almeselmani, M., Deshmukh, P.S., Sairam, R.K., Kushwaha, S.R., Singh, T.P. (2006). Protective role of antioxidant enzymes under high temperature stress. Plant Science, 171(3), 382–388.

Antonić, D., Milošević, S., Cingel, A., Lojić, M., Trifunović-Momčilov, M., Petrić, M., Subotić, A., Simonović, A. (2016). Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought. South African Journal of Botany, 105, 226–233.

Basu, S., Roychoudhury, A., Saha, P.P, Sengupta D.N. (2010). Differential antioxidative responses of indica rice cultivars to drought stress. Plant Growth Regulation, 60(1), 51–59.

Benzie, I.F., & Strain, J.J. (1996). Ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Analytical Biochemistry, 239(1), 70–76.

Berwal, M.K. & Ram, C. (2018). Superoxide Dismutase: A Stable Biochemical Marker for Abiotic Stress Tolerance in Higher Plants. In A. B. De Oliveira (Ed), Abiotic and Biotic Stress in Plants. London, UK: IntechOpen.

Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248–254.

Chance, B., & Maehly A.C. (1955). Assay of catalases and peroxidases. Methods in Enzymology, 2, 764–775.

Chugh, V, Kaur, N, Grewal, M.S., Gupta, A.K. (2013). Differential antioxidative response of tolerant and sensitive maize (Zea mays L.) genotypes to drought stress at reproductive stage. Indian Journal of Biochemistry and Biophysics, 50(2), 150–158.

Cramer, G.R., Urano, K., Delrot, S., Pezzotti, M., Shinozaki, K. (2011). Effects of abiotic stress on plants: a systems biology perspective. BMC Plant Biology, 11, 163.

Dibacto, R.E.K., Tchuente,B.R.T., Nguedjo, M.W., Tientcheu, Y.M.T., Nyobe, E. C., Edoun, F.L.E., Kamini, M.F.G., Dibanda, R.F., Medoua, G.N. (2021): Total polyphenol and flavonoid content and antioxidant capacity of some varieties of Persea americana peels consumed in Cameroon. Scientific World Journal, 2021, e8882594.

Fahad, S., Bajwa. A.A., Nazir, U., Anjum, S.A., Farooq, A., Zohaib, A., Sadia, S., Nasim, W., Adkins, S., Saud, S., Ihsan, M.Z., Alharby, H., Wu, C., Wang, D., Huang, J. (2017). Crop production under drought and heat stress: Plant responses and management options. Frontiers in Plant Science, 8, e1147.

Gill, S.S., & Tuteja, N. (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48(12), 909–930.

Hasanuzzaman, M., Bhuyan, M., Anee, T.I., Parvin, K., Nahar, K., Mahmud, J.A., Fujita, M. (2019). Regulation of ascorbate-glutathione pathway in mitigating oxidative damage in plants under abiotic stress. Antioxidants, 8(9), 384.

Kasote, D.M., Katyare, S.S., Hegde, M.V., Bae, H. (2015). Significance of antioxidant potential of plants and its relevance to therapeutic applications. International Journal of Biological Sciences, 11(8), 982–991.

Kim, Y.H., Khan, A.L., Kim, D.H., Lee, S.Y., Kim, K.M., Waqas, M., Jung, H.Y., Shin, J.H., Kim, J.G., Lee, I.J. (2014). Silicon mitigates heavy metal stress by regulating P-type heavy metal ATPases, Oryza sativa low silicon genes, and endogenous phytohormones. BMC Plant Biology, 14, 13.

Liang, T., Yue, W., Li, Q. (2010): Comparison of the phenolic content and antioxidant activities of Apocynum venetum L. (Luo-Bu-Ma) and two of its alternative species. International Journal of Molecular Sciences, 11(11):4452–4464.

Mehla, N., Sindhi, V., Josula, D., Bisht, P., Wani, S.H. (2017). An introduction to antioxidants and their roles in plant stress tolerance. In M. I. R. Khan & N. A. Khan (Eds.), Reactive Oxygen Species and Antioxidant Systems in Plants: Role and Regulation under Abiotic Stress (pp. 1–23). Singapure, SG: Springer.

Nakano, Y., & Asada K. (1981). Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiology, 22(5), 867–880.

Ough, C.S., & Amerine, M.A. (1988). Methods for Analysis of Musts and Wines (pp. 196–221). New York, NY: John Wiley & Sons.

Sabzmeydani, E., Sedaghathoor, S., Hashemabadi, D. (2021). Effect of salicylic acid and progesterone on physiological characteristics of Kentucky bluegrass under salinity stress. Revista de Ciencias Agrícolas, 38(1), 111–124.

Šamec, D., Karalija, E., Šola, I., Vujčić Bok, V., Salopek-Sondi, B. (2021). The role of polyphenols in abiotic stress response: The influence of molecular structure. Plants 10(1), 18.

Schomburg, I, Jeske, L, Ulbrich, M, Placzek, S., Chang, A., Schomburg, D. (2017). The BRENDA enzyme information system–from a database to an expert system. Journal of Biotechnology 261, 194–206.

Sharma, A., Shahzad, B., Rehman, A., Bhardwaj, R., Landi, M., Zheng, B. (2019). Response of phenylpropanoid pathway and the role of polyphenols in plants under abiotic stress. Molecules (Basel, Switzerland), 24(13), 2452.

Sharma, P., Jha, A.B., Dubey, R.S., Pessarakli, M. (2012). Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany, 2012, e217037.

Smejkal G.B., & Kakumanu S. (2019). Enzymes and their turnover numbers. Expert Review of Proteomics, 16(7), 543–544.

Smirnoff, N., & Arnaud, D. (2019). Hydrogen peroxide metabolism and functions in plants. New Phytologist, 221(3), 1197–1214.

Tola, A.J., Jaballi, A., Missihoun, T.D. (2021). Protein carbonylation: Emerging roles in plant redox biology and future prospects. Plants, 10(7), e1451.

Wang, X, Liu, H, Yu, F, Hu, B, Jia, Y, Sha, H, Zhao, H. (2019). Differential activity of the antioxidant defence system and alterations in the accumulation of osmolyte and reactive oxygen species under drought stress and recovery in rice (Oryza sativa L.) tillering. Scientific Reports 9(1), 8543.

Willekens, H., Chamnongpol, S., Davey, M., Schraudner, M., Langebartels, C., Van Montagu, M., Inzé, D., Van Camp, W. (1997). Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants. The EMBO journal, 16(16), 4806–4816.

Zhishen, J., Mengcheng, T., Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64(4), 555–559.




How to Cite

MATIJEVIĆ, A., ŠAKONJIĆ, A., & MURTIĆ, S. (2022). Antioxidant response of Impatiens walleriana L. to drought. Acta Agriculturae Slovenica, 118(4), 1–7.



Original Scientific Article

Most read articles by the same author(s)