Screening and identification of IAA-capable and cellulose-degrading bacteria with the potential for plant growth-promoting traits

Mai Van DINH; Quang Trung DO; Trong Tri NGUYEN

doi:10.14720/aas.2024.120.2.13447

Authors

Mai Van DINH University of Science, Vietnam National University Hanoi, Hanoi
Quang Trung DO Faculty of Biotechnology, Dai Nam University, Hanoi
Trong Tri NGUYEN Vietnam National University of Forestry, Hanoi

DOI:

https://doi.org/10.14720/aas.2024.120.2.13447

Keywords:

Bacillus sp., cellulose, organic matter, peanut plant, PGPR

Abstract

Strains with both straw degradation and plant growth promotion ability were selected from the cultivated soil in Bac Kan, Vietnam to solve the problems of poor soil microbial diversity status, weak corrosion promotion effect, and poor crop growth caused by fungal rot diseases. Among seventeen bacteria isolated, strain NR1 presented the highest value for cellulase enzyme activity (Hydrolysis index = 24.8 mm), and IAA production (20.15 mg l^-1), and was identified as Bacillus amyloliquefaciens Priest et al., 1987. Inoculation with NR1 significantly increased the rot promotion rate of straw under liquid fermentation by 54.71 % compared with the control and increased the root length and average diameter, and SPAD value of maize under soil culture by 18.3 %, 22.0 %, and 5.24 % respectively (p < 0.05). In addition, fertilizing 8 or 9 tons of NR1-degraded compost fertilizer per hectare had the best effect on the growth, development, and productivity of the L14 peanut variety. These results suggest strain NR1 could be used to produce multi-functional humus, accelerate the decomposition of straw in the cultivated soil, and promote crop growth.

References

Bach, E.M., Baer, S.G., Meyer, C.K., Six, J. (2010). Soil texture affects soil microbial and structural recovery during grassland restoration. Soil Biology and Biochemistry, 42(12), 2182-2191. https://doi.org/10.1016/j.soilbio.2010.08.014

Bandick, A.K. & Dick, R.P. (1999). Field management effects on soil enzyme activities. Soil Biology & Biochemistry, 31, 1471–1479. https://doi.org/10.1016/S0038-0717(99)00051-6

Chen, J.J., Wang, F.W., Liu, M. (2014). Isolation of cellulose-decomposing fungi from the soil and analyzing its cellulase activity. Journal of Anhui Agricultural University, 41(4), 654-661.

Dodor, D.E. & Tabatabai, M.A. (2003). Amidohydrolases in soils as affected by cropping systems. Applied Soil Ecology, 24, 73–90. https://doi.org/10.1016/S0929-1393(03)00067-2

Don, A., Böhme, I. H., Dohrmann, A. B., Poeplau, C., Tebbe, C.C. (2017). Microbial community composition affects soil organic carbon turnover in mineral soils. Biology and Fertility of Soils, 53(4), 445-456. https://doi.org/10.1007/s00374-017-1198-9

Do, Q. T., Nguyen, T. T., Dinh M. V. (2023). Application of endophytic bacterium Bacillus velezensis BTR11 to control bacterial leaf blight disease and promote rice growth. Egyptian Journal of Biological Pest Control, 33, 97. https://doi.org/10.1186/s41938-023-00740-w

Giller, K.E., Witter, E., McGrath, S.P. (1998). Toxicity of heavy metals to microorganisms and microbial processes in agricultural soils: A review. Soil Biology and Biochemistry, 30(10-11), 1389-1414. https://doi.org/10.1016/S0038-0717(97)00270-8

Fierer, N., Strickland, M.S., Liptzin, D. (2009). Global patterns in belowground communities. Ecology Letters, 12(11),1238–1249. https://doi.org/10.1111/j.1461-0248.2009.01360.x

Fontaine, S., Bardoux, G., Benest, D. (2004). Mechanisms of the priming effect in a savannah soil amended with cellulose. Soil Science Society of America Journal, 68, 125–131. https://doi.org/10.2136/sssaj2004.1250

Iqbal, A. (2008). Potensi kompos dan pupuk kandang untuk produksi padi organik pada tanah inceptisol. Journal of Akta Agrosia, 11(1), 13-18.

Jiang, X.J., Huang, Z.X., Xie, D.T. (2000). Promoting effects of the metabolites of silicate for plant growth. Journal of Southwest Agricultural University, 22(2), 116-119.

Li, L.C., Zhang, C., Dong, Q. (2019). Isolation and identification of cellulose-degrading microorganisms in composting process. Biotechnology Bulletin, 35(9), 165-171.

Liang, Y., Zhang, Z., Wu, M. (2014). Isolation, screening, and identification of cellulolytic bacteria from natural reserves in the subtropical region of China and optimization of cellulase production by Paenibacillus terrae ME27-1. Biomed Research International, 2014(5), 512497. https://doi.org/10.1155/2014/512497

Liu, Y., Liu, X.D., Zhang, L.L. (2017). Screening, identification of multifunctional peanut root-promoting rhizobacteria and its promoting effects on peanuts. Biotechnology Bulletin, 33(10), 125-134. doi: 10.13560/j.cnki.biotech.bull.1985.2017-0233

Lu, Y.F., Xu, H., Duan, H.T. (2019). Foliar spraying of liquid compound microbial fertilizer containing Bacillus amyloliquefaciens SQR9 promoted plant growth. Soils, 51(5), 903-909.

Luo, T., Xia, H., Feng, D.S., Gao, H., Wang, Y. (2018). Isolation and identification of multi-function degradation bacteria from forest soil. Southwest China Journal of Agricultural Sciences, 31(5), 1032- 1040.

Madejon, E., Lopez, R., Murillo, J.M., Cabrera, F. (2001). Agricultural use of three (sugar-beet) vinasse composts: Effect on crops and chemical properties of cambisol soil in the Gauadalquivir river valley (SW Spain). Agriculture, Ecosystems & Environment, 84, 55-65. https://doi.org/10.1016/S0167-8809(00)00191-2

QCVN 01-56:2011/BNNPTNT. (2011). National Technical Regulation on Testing for Value of Cultivation and Use of Groundnut Varieties (in Vietnamese).

QCVN 01-57:2011/BNNPTNT. (2011). National Technical Regulation on Testing for Value of Cultivation and Use of Groundnut Varieties (in Vietnamese).

Sardans, J., Penuelas, J., Estiarte, M. (2008). Changes in soil enzymes related to C and N cycle and in soil C and N content under prolonged warming and drought in a Mediterranean shrubland. Applied Soil Ecology, 39(2), 223–235. https://doi.org/10.1016/j.apsoil.2007.12.011

Shankar, T.J., Sokhansanj, S., Hess, J.R., Wright, C.T., Boardman, R.D. (2011). A review on biomass torrefaction process and product properties for energy applications. Industrial Biotechnology, 7(5), 384–401. https://doi.org/10.1089/ind.2011.7.384

Sun, X., Dong, Y.H., Wang, N. (2020). Screening and evaluation of saline-alkali-tolerant and growth-promoting bacteria. Chinese Journal of Biotechnology, 36(7), 1356-1364. doi: 10.13345/j.cjb.190519

Tran, T.A.T., Tran, T.N.S., Nguyen, N.N., Luu, H.M. (2011). Influence of composted rice straw treated by Trichoderma sp. Fungi on rice yield, rice soil fertility and economic effectiveness in summer - autumn 2010 in the Mekong delta. Vietnam Journal of Agriculure and Rural Development, 4, 23-31 (in Vietnamese).

Talbot, J.M., Treseder, K.K. (2012). Interactions among lignin, cellulose, and nitrogen drive litter chemistry-decay relationships. Ecology, 93, 345–354. https://doi.org/10.1890/11-0843.1

Tolan, J.S., Foody, B. (1999). Cellulase from submerged fermentation. In: Recent progress in bioconversion of Lignocellulosics. Advances in Biochemical Engineering/Biotechnology, 65. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-49194-5_3

TCVN 6498:1999. (1999). Soil quality – Determination of total nitrogen – Modified Kjeldahl method (in Vietnamese).

TCVN 8941:2011. (2011). Soil quality – Determination of total organic carbon – Walkley Black method (in Vietnamese).

TCVN 8940:2011. (2011). Soil quality – Determination of total phosphorus – Colorimetry method (in Vietnamese).

TCVN 8660:2011. (2011). Soil quality – Method for determination of total potasium (in Vietnamese).

Ulrich, A., Klimke, G., Wirth, S. (2008). Diversity and activity of cellulose-decomposing bacteria, isolated from a sandy and a loamy soil after long-term manure application. Microbial Ecology, 55(3), 512–522. https://doi.org/10.1007/s00248-007-9296-0

Wang, Q., Wang, L.F., Yan, D.R. (2016). The optimization of enzyme complex formulation for enzymatic hydrolysis of corn stalk. Biotechnology Bulletin, 32(3), 171-177.

Wei, Z., Xu, C.M., Zheng, H.P. (2015). Degradation of rice straw by degrading strains isolated by a “hanging-enrichment” method. Journal of Agro-Environment Science, 34(10), 2027-2031.

Wu, K.C., Liang, J., Wei, L.P. (2011). Effects of nitrogen fixing bacteria on photosynthesis and chlorophyll fluorescence in sugarcane at elongating stage. Guihaia, 31(5), 668-673.

Xi, L.Q., Yao, T., Yang, J.J. (2005). Property of associative nitrogen-fixing bacteria producing IAA and its promoting growth of oat. Grassland and Turf, 2005(4), 25-29.

Yue, B.H., Liao, X.R., Zheng, X.J. (2005). Metabolism and function of indole acetic acid in plant cells. Biological Bulletin, 40(4), 21-23.

Yu, J., Yu, Z.H., Liu, X.B. (2015). Diversity and growth promoting effects of endophytic bacteria in plant root. Chinese Agricultural Science Bulletin, 31(13), 169-175.

Yu, H.J., Guo, X.L. (2019). Screening of straw-degrading bacteria and study on their cellulose-degrading performance. Biotechnology Bulletin, 35(2), 58-63.

Zhang, H., Wang, H.F., Li, C.H. (2018). Effect of exogenous pressure on growth and physiological characteristics of sweet potato. Journal of Jiangsu Normal University: Natural Science Edition, 36(4), 36-43.