Comparative assessment of ISSR, DAMD and RAPD markers for evaluation of genetic diversity of gerbera (Gerbera jamesonii Bolus ex Hooker f.) cultivars

Authors

  • Abbas SAIDI Faculty of Life Sciences and Biotechnology, Department of Plant Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
  • Melika HAJKAZEMIAN Faculty of Life Sciences and Biotechnology, Department of Plant Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

DOI:

https://doi.org/10.14720/aas.2023.119.1.2425

Keywords:

Gerbera, DAMD, genetic diversity, ISSR

Abstract

Genetic diversity is the best way to improve available genetic resources for breeding programs in gerbera. In present study, genetic diversity of 22 gerbera cultivars was investigated using inter-simple sequence repeat (ISSR), directly amplified minisatellite DNA (DAMD), and Random Amplified Polymorphic DNA (RAPD) markers. Average polymorphism information content (PIC) for ISSR, DAMD and RAPD markers was 0.40, 0.41 and 0.40, respectively. Cluster analysis for ISSR, DAMD and RAPD divided the cultivars into three distinct clusters. The comparative analysis of the three markers (ISSR, DAMD and RAPD) showed that DAMD had superiority over RAPD and ISSR in characterization of genetic diversity in Gerbera. To our knowledge, this is the first report of a comparison of performance among DAMD, ISSR and RAPD techniques on a set of gerbera genotypes. Overall, our results showed that DAMD markers well represented different genotypes of gerbera diversity.

References

Aghdam, M., Asil, M. H., Ghasemnezhad, M., & Mirkalaei, S. A. A. M. (2019). Effects of pre-harvest applications of different source of calcium on the cell wall fractions and stem bending disorder of Gerbera (Gerbera jamesonii L.) cultivar flowers. Advances in Horticultural Science, 33(1), 57-65.

Danaee, E., Mostofi, Y., & Moradi, P. (2011). Effect of GA3 and BA on postharvest quality and vase life of gerbera (Gerbera jamesonii. cv. Good Timing) cut flowers. Horticulture, Environment, and Biotechnology, 52(2), 140-144. https://doi.org/10.1007/s13580-011-1581-7

Feghhi, S. M. A., Norouzi, P., Saidi, A., Zamani, K., & Amiri, R. (2012). Identification of SCAR and RAPD markers linked to Rz1 gene in sugar beet using BSA and two genetic distance estimation methods. Electronic Journal of Plant Breeding, 3(1), 598-605.

Gong, L., & Deng, Z. (2010). EST-SSR markers for gerbera (Gerbera hybrida). Molecular Breeding, 26(1), 125-132. https://doi.org/10.1007/s11032-009-9380-x

Hajibarat, Z., Saidi, A., Hajibarat, Z., & Talebi, R. (2014). Genetic diversity and population structure analysis of landrace and improved chickpea (Cicer arietinum) genotypes using morphological and microsatellite markers. Environmental and Experimental Biology, 12(4), 161-166.

Khodadadi, M., Fotokian, M. H., & Miransari, M. (2011). Genetic diversity of wheat (Triticum aestivum L.) genotypes based on cluster and principal component analyses for breeding strategies. Australian Journal of Crop Science, 5(1), 17-24.

Kumar, S., Kumaria, S., Sharma, S. K., Rao, S. R., & Tandon, P. (2011). Genetic diversity assessment of Jatropha curcas L. germplasm from Northeast India. Biomass and Bioenergy, 35(7), 3063-3070. https://doi.org/10.1016/j.biombioe.2011.04.025

Lassner MW, Peterson P, Yoder JI (1989) Simultaneous amplification of multiple DNA fragments by polymerase chain reaction in the analysis of transgenic plants and their progeny. Plant Molecular Biology Reporter, 7, 116–128. https://doi.org/10.1007/BF02669627

Li, Z. J., Xiong, L., & Gui, M. (2004). Optimization of the genomic DNA extraction and concentration of DNA template for ISSR-PCR amplification of Gerbera jamesonii cultivars. Acta Botanica Yunnanica, 26(4), 439-444.

Mantel, N. (1967). The detection of disease clustering and a generalized regression approach. Cancer Research, 27(2_Part_1), 209-220.

Pakseresht, F., Talebi, R., & Karami, E. (2013). Comparative assessment of ISSR, DAMD and SCoT markers for evaluation of genetic diversity and conservation of landrace chickpea (Cicer arietinum L.) genotypes collected from north-west of Iran. Physiology and Molecular Biology of Plants, 19(4), 563-574. https://doi.org/10.1007/s12298-013-0181-7

Prajapati, P., Singh, A., Patel, N.L., Singh, D., & Srivastav, V. (2014). Evaluation of genetic diversity in different genotypes of Gerbera jamesonii Bolus using random amplified polymorphic DNA (RAPD) markers. African Journal of Biotechnology, 13, 1117-1122. https://doi.org/10.5897/AJB2013.13347

Rafalski, J.A. (2002). Novel genetic mapping tools in plants: SNPs and LD-based approaches. Plant Science, 162, 329–333. https://doi.org/10.1016/S0168-9452(01)00587-8

Saidi, A., Eghbalnegad, Y. & Hajibarat, Z. (2017). Study of genetic diversity in local rose varieties (Rosa spp.) using molecular markers. Banat′s Journal of Biotechnology, 16, 148-157. https://doi.org/10.7904/2068-4738-VIII(16)-148

Saidi, A, Daneshvar, Z. & Hajibarat, Z. (2018). Comparison of genetic variation of Anthurium (Anthurium andraeanum) cultivars using SCoT, CDDP and RAPD markers. Plant Tissue Culture and Biotechnology, 28, 171-82. https://doi.org/10.3329/ptcb.v28i2.39676

Seyedimoradi, H. & Talebi, R. (2014). Detecting DNA polymorphism and genetic diversity in lentil (Lens culinaris Medik.) germplasm: comparison of ISSR and DAMD marker. Physiology and Molecular Biology of Plants, 20, 495 –500. https://doi.org/10.1007/s12298-014-0253-3

Yang, X., Pang, H. B., Liu, B. L., Qiu, Z. J., Gao, Q., Wei, L., ... & Wang, Y. Z. (2012). Evolution of double positive autoregulatory feedback loops in CYCLOIDEA2 clade genes is associated with the origin of floral zygomorphy. The Plant Cell, 24(5), 1834-1847. https://doi.org/10.1105/tpc.112.099457

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Published

28. 04. 2023

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Original Scientific Article

How to Cite

SAIDI, A., & HAJKAZEMIAN, M. (2023). Comparative assessment of ISSR, DAMD and RAPD markers for evaluation of genetic diversity of gerbera (Gerbera jamesonii Bolus ex Hooker f.) cultivars. Acta Agriculturae Slovenica, 119(1), 1–8. https://doi.org/10.14720/aas.2023.119.1.2425

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