Standardization of detached leaf assay to screen chickpeas for resistance to beet armyworm, Spodoptera exigua (Hübner, 1808)

Authors

  • Shankar MARLABEEDU International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru and Acharya N.G. Ranga Agricultural University (ANGRAU), Andhra Pradesh, India
  • Suraj Prasad MISHRA International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India
  • Hari Chanda SHARMA International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India
  • Jagadish JABA International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India
  • Ramesh Babu TATINENI Acharya N.G. Ranga Agricultural University (ANGRAU), Andhra Pradesh, India
  • Ramesh Babu TATINENI Acharya N.G. Ranga Agricultural University (ANGRAU), Andhra Pradesh, India
  • Sridevi DASARI Acharya N.G. Ranga Agricultural University (ANGRAU), Andhra Pradesh, India

DOI:

https://doi.org/10.14720/aas.2023.119.1.2682

Keywords:

Spodoptera exigua, chickpea, host plant resistance, screening technique, detached leaf assay

Abstract

The beet armyworm, Spodoptera exigua (Hübner, 1808) is an important pest of several economically important crops, and recently emerged as a serious pest of chickpea in South Central India. We standardized a detached leaf assay technique to evaluate chickpea germplasm and segregating populations for resistance to this pest under laboratory conditions. Two chickpea genotypes ICCL 86111 and ICC 3137 grown under field and greenhouse conditions were used for the detached leaf assay at the vegetative and flowering stages. The terminal branches were infested with 5, 10, 15, and 20 neonate larvae of S. exigua. The test genotypes were also infested with 2, 4, 6 and 8 third-instar larvae at the podding stage. At the vegetative stage, ICCL 86111 suffered less damage than ICC 3137 across infestation levels. The differences in larval survival between the genotypes were significant, and larval survival was lower on ICCL 86111 than on ICC 3137 across infestation levels. The results suggested that infesting the chickpea terminal branches with 10–15 neonate larvae per branch at the vegetative stage or six third-instar larvae at the podding stage can be used to evaluate chickpea genotypes for resistance to S. exigua.

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Published

28. 04. 2023

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Section

Original Scientific Article

How to Cite

MARLABEEDU, S., MISHRA, S. P., SHARMA, H. C., JABA, J., TATINENI, R. B., TATINENI, R. B., & DASARI, S. (2023). Standardization of detached leaf assay to screen chickpeas for resistance to beet armyworm, Spodoptera exigua (Hübner, 1808). Acta Agriculturae Slovenica, 119(1), 1–8. https://doi.org/10.14720/aas.2023.119.1.2682

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