Preliminary assessment of genetic diversity between Glebionis coronaria and G. discolor (Asteraceae) by AFLP markers

Alessia GALLUCCI; Carmelo Maria MUSARELLA; Ana CANO-ORTIZ; José Carlos PIÑAR FUENTES; Ricardo QUINTO CANAS; Clizia VILLANO

doi:10.14720/aas.2023.119.1.14391

Authors

Alessia GALLUCCI Department of Agricultural Sciences, University of Naples Federico II, Naples, Italy
Carmelo Maria MUSARELLA Department of Agriculture, Mediterranean University of Reggio Calabria, Reggio Calabria, Italy
Ana CANO-ORTIZ Department of Didactics of Experimental Social Sciences and Mathematics, Section of Didactics of Experimental Sciences, Faculty of Education, Complutense University of Madrid, Madrid, Spain
José Carlos PIÑAR FUENTES Department of Animal and Plant Biology and Ecology, Section of Botany, University of Jaen, Jaén, Spain
Ricardo QUINTO CANAS Faculty of Sciences and Technology, University of Algarve, Faro and Centre of Marine Sciences (CCMAR), University of Algarve, Faro, Portugal
Clizia VILLANO Department of Agricultural Sciences, University of Naples Federico II, Naples, Italy

DOI:

https://doi.org/10.14720/aas.2023.119.1.14391

Keywords:

biodiversity, Compositae, Chrysanthemum, daisy, garland chrysanthemum, taxonomy

Abstract

Glebionis coronaria is a valuable and medicinal herb native of Mediterranean region. Recently, G. coronaria var. discolor has been elevated to the rank of species as G. discolor (d’Urv.) Cano based on morphological characteristics, distinguishing it from G. coronaria var. coronaria (=G. coronaria). To investigate the genetic basis of this diversity, AFLP markers were applied to genotypes of G. discolor and G. coronaria sampled in three different Mediterranean regions (Italy, Spain, and Portugal). Our results showed that among 1347 fragments identified with five primer combinations 99.55 % were polymorphic. The genetic distance and the Shannon Index values suggested that the two species can be genetically distinguished, but further studies are needed to confirm this hypothesis.

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