Setting up a gene expression study for tissue cells by method of quantitative real-time PCR

Authors

  • Daliborka DUŠANIĆ Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia
  • Luka BOLHA Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia
  • Mojca NARAT Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia
  • Irena OVEN Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia

DOI:

https://doi.org/10.14720/aas.2012.100.1.14417

Keywords:

molecular genetics, RT-qPCR, gene expression, tissue cells

Abstract

Gene expression analysis by quantitative reverse transcription polymerase chain reaction (RT-qPCR) allows accurate and sensitive measurment of gene expression levels. However, a series of steps needs to be taken to ensure the relevance, accuracy, correct interpretation and repeatability of the RT-qPCR experiment. We describe here a simple experiment of determining relative gene expression for caspase 8 gene in chicken chondrocytes treated with an apoptosis inducing compound 5-fluorouracil. We use this example to point out some important guidelines in setting up a gene expression study in tissue cells, analyzing and interpreting the results and reporting on the findings.

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Published

13. 07. 2012

Issue

Section

Original Scientific Article

How to Cite

DUŠANIĆ, D., BOLHA, L., NARAT, M., & OVEN, I. (2012). Setting up a gene expression study for tissue cells by method of quantitative real-time PCR. Acta Agriculturae Slovenica, 100(1), 19–28. https://doi.org/10.14720/aas.2012.100.1.14417

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