Using a different growth medium greatly improves distinction of Butyrivibrio fibrisolvens and Pseudobutyrivibrio xylanivorans strains by the cellular fatty acids and aldehydes profiles

Maša ZOREC; Maša VODOVNIK; Romana MARINŠEK LOGAR

doi:10.14720/aas.2011.98.1.14591

Using a different growth medium greatly improves distinction of Butyrivibrio fibrisolvens and Pseudobutyrivibrio xylanivorans strains by the cellular fatty acids and aldehydes profiles

Authors

Maša ZOREC Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia
Maša VODOVNIK Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia
Romana MARINŠEK LOGAR Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia

DOI:

https://doi.org/10.14720/aas.2011.98.1.14591

Keywords:

microbiology, anaerobic bacteria, rumen, Butyrivibrio, Pseudobutyrivibrio, FAME, DMA

Abstract

A total of 11 ruminal strains currently assigned to Butyrivibrio fibrisolvens and Pseudobutyrivibrio xylanivorans were cultivated in two different media, rumen fluid containing M2 and short-chain fatty acid (SCFA) containing M330, and their cellular fatty acid methyl esters (FAME) and dimethylacetals (DMA) were analyzed using gas chromatography. A comparison of the FAME/DMA compositions revealed that the difference in SCFA contents in the growth medium induced a pronounced quantitative effect on the cellular branched-chain fatty acid and aldehydes proportions only in the P. xylanivorans strains. This study shows that FAME/DMA analysis is a powerful chemotaxonomic tool in the group of phenotypically similar rumen butyrivibria especially when the influence of the growth medium is evaluated.

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Published

27. 07. 2011

Issue

Vol. 98 No. 1 (2011): ANIMAL PRODUCTION

Section

Original Scientific Article

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

ZOREC, M., VODOVNIK, M., & MARINŠEK LOGAR, R. (2011). Using a different growth medium greatly improves distinction of Butyrivibrio fibrisolvens and Pseudobutyrivibrio xylanivorans strains by the cellular fatty acids and aldehydes profiles. Acta Agriculturae Slovenica, 98(1), 15–19. https://doi.org/10.14720/aas.2011.98.1.14591