Investigating the effects of plant growth-promoting rhizobacteria isolates on germination and physiology status of durum wheat under salt stress

Khaled BOUFARES; Mostefa KOUADRIA; Mohamedi KARIMA; Yahia Naima MERDJET

doi:10.14720/aas.2023.119.2.2224

Authors

Khaled BOUFARES University of Tiaret, Faculty of Life and Natural Sciences, Agronomy Department, Tiaret; Agro-biotechnology and nutrition in semi-arid zones Laboratory (LANZA), Tiaret, Algeria
Mostefa KOUADRIA University of Tiaret, Faculty of Life and Natural Sciences, Agronomy Department, Tiaret; Agro-biotechnology and nutrition in semi-arid zones Laboratory (LANZA), Tiaret, Algeria
Mohamedi KARIMA Laboratory of Plant Physiology Applied to Soilless Crops, Tiaret, Algeria
Yahia Naima MERDJET Laboratory of Plant Physiology Applied to Soilless Crops, Tiaret, Algeria

DOI:

https://doi.org/10.14720/aas.2023.119.2.2224

Keywords:

bacterial inoculation, biofertilization, PGPR, plant-microbe interactions, omoregulators, salt stress, durum wheat

Abstract

The aim of this work is to evaluate the seedling growth and physiology status of wheat seeds inoculated with a suspension of eight plant growth-promoting rhizobacteria (PGPR) isolates. For this purpose, rhizobacteria strains were isolated from the roots of native plants growing in the Algerian steppe, then evaluated for their plant growth promotion (PGP) features, and finally applied on wheat seeds. The obtained results showed that the majority of the tested strains displayed pertinent PGP features. In in vitro experiments, results showed that salinity affected negatively seed germination and impaired plant growth while the inoculation with BC3, BC6 and BC7 strains induced a good germination rate and improved significantly the root length. In greenhouse experience, data demonstrated that non-inoculated plants accumulated a significant amount of osmoregulators (proline and glycine betaine), and recorded a decrease of their chlorophyll content, compared to inoculated plants, where the salinity tolerance of this latter has been much better with a high seedling growth as well as high chlorophyll and low osmolyte contents. The results may be a useful extension of our knowledge of the interaction between plant and PGPR, in view of their possible applications as a biofertilizer to improve plant growth in salinity-impacted regions.

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