Methylmercury inhibits growth and induces membrane changes in Pseudomonas putida

Authors

  • Maša VODOVNIK Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia
  • Mirjana BISTAN Laboratory for environmental science and engineering, National institute of chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia
  • Maša ZOREC Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia
  • Romana MARINŠEK LOGAR Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia

DOI:

https://doi.org/10.14720/aas.2010.96.2.14702

Keywords:

microbiology, environmental protection, bacteria, Pseudomonas putida, aquatic ecosystems, pollution, mercury, methylmercury, growth inhibition, membrane adaptation, cis-trans isomerization

Abstract

A bacterial model system (Pseudomonas putida DSM 50026) was used in this research to assess toxicity of the environmentally relevant concentrations of mercury species (MeHg and Hg(II)) that represent important pollutants of aquatic ecosystems at sites of industrial or mining activities. In addition to direct monitoring of bacterial growth, we also analyzed fatty acid profiles of exposed and non-exposed cultures to determine possible toxic effects manifested on membrane level. The results showed that exposure of P. putida to Hg(II) in concentrations of 0.2–200 µg/L did not have any significant effects on growth nor fatty acid composition of exposed bacterial culture. On the other hand, when bacteria were exposed to up to 1600-times lower concentrations of MeHg (0.12–12.5 µg/L), growth inhibition as well as significant changes in fatty acid composition were detected. Observed adaptive membrane changes due to MeHg exposure were similar to those associated with responses to organic solvents and some other membrane-disrupting compounds.

Downloads

Published

27. 12. 2010

Issue

Section

Original Scientific Article

How to Cite

VODOVNIK, M., BISTAN, M., ZOREC, M., & MARINŠEK LOGAR, R. (2010). Methylmercury inhibits growth and induces membrane changes in Pseudomonas putida. Acta Agriculturae Slovenica, 96(2), 87–93. https://doi.org/10.14720/aas.2010.96.2.14702

Similar Articles

1-10 of 669

You may also start an advanced similarity search for this article.