Results of testing of the efficacy of sublethal concentrations of bacterial-chemical insecticides combinations against cabbage moth larvae

Hrant TERLEMEZYAN; Masis SARGSYAN; Harutyun HARUTYUNYAN; Noushig ZARIKIAN; Sona SARGSYAN; Gabriel KARAPETYAN; Habetnak MKRTCHYAN

doi:10.14720/aas.2023.119.3.14714

Authors

Hrant TERLEMEZYAN Research Centre of Risk Assessment and Analysis in Food Safety Area, Yerevan, Armenia
Masis SARGSYAN Research Centre of Risk Assessment and Analysis in Food Safety Area, Yerevan, Armenia
Harutyun HARUTYUNYAN Research Centre of Risk Assessment and Analysis in Food Safety Area, Yerevan, Armenia
Noushig ZARIKIAN Department of Experimental Zoology, Scientific Center of Zoology and Hydroecology of NAS RA, Yerevan, Armenia
Sona SARGSYAN Research Centre of Risk Assessment and Analysis in Food Safety Area, Yerevan, Armenia
Gabriel KARAPETYAN Research Centre of Risk Assessment and Analysis in Food Safety Area, Yerevan, Armenia
Habetnak MKRTCHYAN Research Centre of Risk Assessment and Analysis in Food Safety Area, Yerevan, Armenia

DOI:

https://doi.org/10.14720/aas.2023.119.3.14714

Keywords:

biological effectiveness, cabbage moth larvae, insecticides, laboratory and field tests, statistical analysis

Abstract

Using chemical pesticides has adverse effects on the environment and humans. Bacterial preparations may provide an alternative to chemical pesticides. The study aims to test different combinations of sublethal concentrations of bacterial and chemical preparations against cabbage moth larvae.

During 2020-2022 different combinations of sublethal concentrations of bacterial (Lepidocide) and chemical (Arrivo, Voliam Flexi, Proclaim Fit) preparations were tested in laboratory and field conditions, against cabbage moth young larvae (stage I-II).

The combinations of insecticides with bacterial and chemical sublethal concentrations show high biological efficiency against the cabbage moth larvae. No statistical difference was found between the efficiency indicators of the combined and standard chemical (Arrivo, Voliam Flexi, Proclaim Fit) options and the significance level was generally between 2.0 and 5.9 %, showing that the results of the scientific experiments are reliable.

References

Andreeva, I.A., Shatalova, E.I. and Khodakova, A.V. (2021). Cabbage moth Plutella xylostella: ecological and biological aspects, harmfulness, population control. Bulletin of Plant Protection, 104(1), 28–39. [In Russian]. https://doi.org/10.31993/2308-6459-2021-104-1-14947

Ashmarin, I.P., &d Vorobyev, A.A. (1962). Statistical methods in microbiological research. Medgiz, 180 p. [In Russian].

Avagyan, A.M. (2012). Development of integrated control on ecological basis against main pests – Horse radish leaf beetle in conditions of Lori marz. Abstr., PhD. Biol. Thesis. Erevan. 23 p. [In Armenian].

Avetyan, H.S. and G.M. Marjanyan (1976). Pests of agricultural crops, forests and warehouses of Armenia. Publishing House of the Academy of Sciences of the Armenian SSR, Yerevan. 832 pp. [In Armenian].

Belyaev, O.V., & Nozdrenko, Ya.V. (2004). Lepidocide against the unpaired silkworm, Protection and Quarantine of Plants, 6, 42-43. [In Russian].

Bernstein, A. (1968). Handbook of Statistical solutions. M.: Statistics, 162 p. [In Russian].

Chapanyan, E.N. (2022). Developing integrated pest management measures against the main forest leaf beetles with the ecologically-based crystal-forming local bacterial insecticides of Aragatsotn region. Abstr. PhD. Biol. Thesis. Erevan. 27 p. [In Armenian].

Churikova, V.G., & Silaev, A.I. (2010). Pests of spring rape in the Lower Volga region, Agro, XXI, (4-6), 24–27. [In Russian]. https://doi.org/10.28983/asj.y2020i11pp71-77

Fathipour, Y., & Mirhosseini, M.A. (2017). Diamondback moth (Plutella xylostella) management. In: G.V.P. Reddy (ed.). Integrated Management of Insect Pests on Canola and Other Brassica Oilseed Crops (pp.13–43). Oxford; Boston, MA: CAB International. https://doi.org/10.1079/9781780648200.0013

Harcourt, D.G. (1957). Biology of the diamondback Moth, Plutella maculipennis (Curt.) (Lepidoptera: Plutellidae), in Eastern Ontario. Canadian Entomologist, 89(12), 554–564. https://doi.org/10.4039/Ent89554-12

Ivantsova, E.A. (2004). Biopreparation against mustard pests, Protection and Quarantine of Plants, 6, 36. [In Russian]. DOI: https://dx.doi.org/10.15688/jvolsiu 11.2015.2.6

Kholod, A.S., & Korenyuk, E.F. (2016). Cabbage moth – a threat to rapeseed crops in the Omsk region, Protection and Quarantine of Plants, 5, 32–33. [In Russian]. https://doi.org/10.28983/asj.y2020i11pp71-77

Mesropyan, H.R. (2011). Effect of BTB and Lepidocide on the microflora and enzymatic activity of afforestation typical chernozem. Abstr. PhD. Biol. Thesis. Erevan. 24 p. [In Armenian].

Methodological guidelines for testing biological products for plant protection from pests, diseases and weeds. 1973. Moscow, Kolos, 112 p. [In Russian].

Netrusov, A.I., Egorov, M. A., & Zakharchuk L.M. (2005). Workshop on microbiology. Moscow: IC Academy, 608 p. [In Russian].

Philips, C.R., Fu, Z., Kuhar, T.P., Shelton, A.M., & Corder,o R.J. (2014). Natural history, ecology, and management of diamondback moth (Lepidoptera: Plutellidae), with emphasis on the United States. Journal of Integrated Pest Management, 5(3), D1–D11. https://doi.org/10.1603/IPM14012

Polyakov, I.Ya. (1984). Forecast of the development of pests and diseases of agricultural crops (with a workshop). L.: “Ear”, Leningrad branch, 318 p. [In Russian].

Safaryan, S.E. (1968). The harmful fauna of cabbage in Armenia and the biological basis for the development of control measures. Abstract of the dis. candidate of agr. sciences. Yerevan, 29 p. [In Russian].

Sarantseva, N.A., & Bobreshova, I. Yu. (2006). Biopreparations against the Colorado potato beetle, Protection and Quarantine of Plants, 7, 27–28. [In Russian].

Sargsyan, A.M. (2013). The biological efficiency of bacterial insecticides isolated from biocenosis against the major leaf beetle pests of the forest and their influence on biological activity of forest brown soils. Abstract of the thesis candidate of biol. sciences. Yerevan, 26 p. [In Armenian].

Semerenko, S.A. (2019). Pheromonitoring of cabbage moth in rapeseed crops and a search of effective chemicals for pest control in the conditions of the Western Ciscaucasia. Oil Crops. Scientific and Technical Bulletin of VNIIMK, 4(180), 143–151. [In Russian]. https://doi.org/10.25230/2412-608X-2019-4-180-143-151

Shpanev, A.M. (2015). Mass reproduction of cabbage moth, Protection and Quarantine of Plants, 9, 40-42. (In Russian). DOI: 10.31857/S0002188122080130.

Talekar, N.S., & Shelton, A.M. (1993). Biology, ecology, and management of the diamondback moth. Annual Review of Entomology, 38(1), 275–301. https://doi.org/10.1146/annurev.en.38.010193.001423

Terlemezyan, H.L. (1996). Harmful fauna of vegetable crops of the Ararat Plain: bioecological features of the main species and integrated control against them. Ph. D. Thesis, Yerevan University, Armenia. 301 pp. [In Russian].

Tsedeler, O.E. (1931). Cabbage moth (Plutella maculipennis Curt.) in connection with cultural mustard, Journal of Experimental Agrochemistry of the South-East, 9(2), 165–195. [In Russian]. https://doi.org/10.28983/asj.y2020i11pp71-77

Tuleeva, A.K., & Sarmanova, R.S. (2019). Pests of spring rape in Akmola region. Protection and Quarantine of Plants, 12, 20–23. [In Russian]. https://doi.org/10.28983/asj.y2020i11pp71-77

Zakharova, Yu.A., Frolov, A.N., & Artemyeva, A.M. (2022). Monitoring of the diamondback moth (Plutella xylostella L.) on the Brassica oleracea L. collection in the vicinity of St. Petersburg. Proceedings on Applied Botany, Genetics and Breeding, 183(4), 219–228. https://doi.org/10.30901/2227-8834-2022-4-219-228