Xylan degradation by different xylanases of rumen bacterium Pseudobutyrivibrio xylanivorans Mz5T

Authors

  • Tadej ČEPELJNIK Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia
  • Katarina FIDLER Volčji Potok 30, SI-1240 Kamnik, Slovenia
  • Romana MARINŠEK-LOGAR Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia

DOI:

https://doi.org/10.14720/aas.2004.84.2.14749

Keywords:

microbiology, anaerobic bacteria, Pseudobutyrivibrio xylanivorans, xylanases, xylan, rumen

Abstract

The use of xylanases as feed additives for monogastric animals is very promising. Many experiments have proved their beneficial effect on animal health and performance. A new rumen bacterium species has recently been isolated: P. xylanivorans, type strain Mz5T, which has a very high xylanolytic activity. Some of its xylanolytic enzymes have already been described. We want to analyse the rest of them to find the reason for its high xylanolytic activity and to assess the possibilities of using this bacterium as a probiotic for monogastric animals (pigs, poultry) or its enzymes as feed additives. We have partially isolated two xylanases from the cell extract: 44 kDa and 81 kDa and examined their properties. We also compared enzyme activities of cell surface xylanases with xylanases in cell extract. The 44 kDa xylanase is an exoxylanase without β-xylosidase activity. We assume that the 81 kDa xylanase is an endoxylanase. Xylanolytic enzymes on the cell surface are efficient xylan degraders, but they are unable to degrade xylobiose. Xylobiose is degraded by xylanolytic enzymes inside the cell.

Published

20. 12. 2004

Issue

Section

Original Scientific Article

How to Cite

ČEPELJNIK, T., FIDLER, K., & MARINŠEK-LOGAR, R. (2004). Xylan degradation by different xylanases of rumen bacterium Pseudobutyrivibrio xylanivorans Mz5T. Acta Agriculturae Slovenica, 84(2), 141–151. https://doi.org/10.14720/aas.2004.84.2.14749

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