Isolation and use of Prevotella ruminicola TC18 plasmid pTC18 in Escherichia coli-P. ruminicola shuttle vector construction

Authors

  • Tomaž ACCETTO Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia

DOI:

https://doi.org/10.14720/aas.2009.94.2.14854

Keywords:

microbiology, anaerobic bacteria, Prevotella ruminicola, shuttle vector, rumen

Abstract

A cryptic plasmid of approximately 3 kilobases named pTC18 was discovered in a ruminal Prevotella ruminicola TC18 strain and cloned into Escherichia coli. Based on pTC18, several shuttle vectors, containing Prevotella/Bacteroides tetQ selection marker and E. coli vector pUC19 inserted at two different positions in pTC18 were constructed. The shuttle vectors, protected with HaeIII methylase against the P. ruminicola 23 restriction were electroporated into P. ruminicola. Despite numerous attempts a tetracycline resistant recombinant strain 23 was not obtained. The possible causes for electroporation failure are discussed.

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Published

11. 12. 2009

Issue

Section

Original Scientific Article

How to Cite

ACCETTO, T. (2009). Isolation and use of Prevotella ruminicola TC18 plasmid pTC18 in Escherichia coli-P. ruminicola shuttle vector construction. Acta Agriculturae Slovenica, 94(2), 143–146. https://doi.org/10.14720/aas.2009.94.2.14854

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