2-D separation of Verticillium albo-atrum proteins

Polona JAMNIK; Sebastjan RADIŠEK; Branka JAVORNIK; Peter RASPOR

doi:10.14720/aas.2006.87.2.15125

Authors

Polona JAMNIK University of Ljubljana, Biotechnical Faculty, Food Science and Technology Department, Chair of Biotechnology, Jamnikarjeva 101, 1000 Ljubljana, Slovenia
Sebastjan RADIŠEK Slovenian Institute of Hop Research and Brewing, Plant Protection Department, Cesta Žalskega tabora 2, 3310 Žalec, Slovenia
Branka JAVORNIK University of Ljubljana, Biotechnical Faculty, Centre for Plant Biotechnology and Breeding, Jamnikarjeva 101, 1000 Ljubljana, Slovenia
Peter RASPOR University of Ljubljana, Biotechnical Faculty, Food Science and Technology Department, Chair of Biotechnology, Jamnikarjeva 101, 1000 Ljubljana, Slovenia

DOI:

https://doi.org/10.14720/aas.2006.87.2.15125

Keywords:

Verticillium albo-atrum, proteomics, 2-D electrophoresis

Abstract

Verticillium albo-atrum is a phytopathogenic fungus that causes wilt disease in a wide range of crops. A proteomic approach is needed to obtain a comprehensive overview of the proteins related to the infection process. We report here on the optimisation of electrophoretic analysis of total proteins of V. albo-atrum in terms of the extraction of fungus total proteins, removal of interfering compounds and separation of proteins by two-dimensional electrophoresis.