Introduction of Grapevine virus B and Grapevine leafroll-associated virus 2 testing in sanitary selection of grapevine

Authors

  • Irma TOMAŽIČ University of Primorska – Science and Research Centre of Koper, SI- 6000 Koper, Garibaldijeva 1, Slovenia
  • Irena MAVRIČ PLEŠKO Agricultural Institute of Slovenia, SI-1000, Ljubljana, Hacquetova 17, Slovenia
  • Nataša PETROVIČ National Institute of Biology, SI-1000, Večna pot 111, Slovenia
  • Maja RAVNIKAR National Institute of Biology, SI-1000, Večna pot 111, Slovenia
  • Zora KOROŠEC-KORUZA University of Ljubljana, Biotechnical Faculty, Department of Agronomy, Jamnikarjeva 101, p.p. 2995, SI-1111, Ljubljana, Slovenia

DOI:

https://doi.org/10.14720/aas.2008.91.1.15169

Keywords:

grapevine, selection, Grapevine virus B, Grapevine leafroll-associated virus-2

Abstract

To introduce testing of Grapevine leafroll-associated virus-2 (GLRaV-2) and Grapevine virus B (GVB) in sanitary selection of grapevine, commercially available antibodies were evaluated and conditions for routine ELISA testing were optimized. Extraction procedure with Granex 91 - special machine, which is used in routine testing in Slovenia, was compared with grinding samples in mortar. Three different extraction buffers were applied in order to overcome the inconvenience of using more than one extraction procedure when testing grapevine material for several viruses in a routine large-scale testing scheme. Results were verified with Western blot and immuno-electron microscopy. The best results were obtained using extraction buffer with unknown composition (pH 9.0) from BIOREBA kit for GLRaV-2. Other extraction buffers gave less positive samples and they are not convenient for routine testing where extraction with Granex is done. Both viruses, GLRaV-2 and GVB were found in Slovenia, but they couldn't be correlated with rougose wood disease that appears on indigenous cultivar Refošk grafted on 'SO4' from collection vineyard in Komen.

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Published

15. 05. 2008

Issue

Section

Original Scientific Article

How to Cite

TOMAŽIČ, I., MAVRIČ PLEŠKO, I., PETROVIČ, N., RAVNIKAR, M., & KOROŠEC-KORUZA, Z. (2008). Introduction of Grapevine virus B and Grapevine leafroll-associated virus 2 testing in sanitary selection of grapevine . Acta Agriculturae Slovenica, 91(1), 75–85. https://doi.org/10.14720/aas.2008.91.1.15169

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