DNA analysis of acidocin lf221 A and B genes, produced by Lactobacillus gasseri LF221

Authors

  • Andreja ČANŽEK MAJHENIČ Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science., Groblje 3, SI-1230 Domžale, Slovenia
  • Irena ROGELJ Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science., Groblje 3, SI-1230 Domžale, Slovenia

DOI:

https://doi.org/10.14720/aas.2003.82.2.15274

Keywords:

microbiology, lactic acid bacteria, Lactobacillus gasseri LF221, bacteriocins, acidocin LF221 A, acidocin LF221 B, molecular genetics, genetic determinants

Abstract

Molecular biology tools were used to locate and analyse genetic determinants coding for acidocin LF221 A and acidocin LF221 B, respectively. 0.9 kb BamHI/HindIII fragment, carrying information for acidocin LF221 A was subjected to the sequencing reaction which revealed two complete ORFs and one incomplete. OrfA2 was determined to code for acidocin LF221 A (Acd221A), while OrfA3 codes a peptide, composed of 90 amino acids which probably acts as an immunity protein. Truncated OrfA1, consisting of 37 amino acids for the moment, represents a putative complementary component (Acd221α) of the acidocin LF221 A. Analysis of the 1.8 kb EcoRI/HindIII fragment, carrying the acidocin LF221 B gene, confirmed the presence of 4 complete ORFs and fifth incomplete. OrfB3, OrfB4 and OrfB5 coding for the complementary component (Acd221β) to acidocin LF221 B, the active acidocin LF221 B (Acd221B) and the putative immunity protein, respectively, are organized in one operon. Analyses of the genetic determinants of the acidocin LF221 A and acidocin LF221 B and their flanking loci revealed that LF221 acidocins are two different, two-peptide bacteriocins produced by L. gasseri LF221.

Published

15. 12. 2003

Issue

Section

Original Scientific Article

How to Cite

ČANŽEK MAJHENIČ, A., & ROGELJ, I. (2003). DNA analysis of acidocin lf221 A and B genes, produced by Lactobacillus gasseri LF221. Acta Agriculturae Slovenica, 82(2), 97–108. https://doi.org/10.14720/aas.2003.82.2.15274

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