DNA analysis of acidocin lf221 A and B genes, produced by Lactobacillus gasseri LF221
DOI:
https://doi.org/10.14720/aas.2003.82.2.15274Keywords:
microbiology, lactic acid bacteria, Lactobacillus gasseri LF221, bacteriocins, acidocin LF221 A, acidocin LF221 B, molecular genetics, genetic determinantsAbstract
Molecular biology tools were used to locate and analyse genetic determinants coding for acidocin LF221 A and acidocin LF221 B, respectively. 0.9 kb BamHI/HindIII fragment, carrying information for acidocin LF221 A was subjected to the sequencing reaction which revealed two complete ORFs and one incomplete. OrfA2 was determined to code for acidocin LF221 A (Acd221A), while OrfA3 codes a peptide, composed of 90 amino acids which probably acts as an immunity protein. Truncated OrfA1, consisting of 37 amino acids for the moment, represents a putative complementary component (Acd221α) of the acidocin LF221 A. Analysis of the 1.8 kb EcoRI/HindIII fragment, carrying the acidocin LF221 B gene, confirmed the presence of 4 complete ORFs and fifth incomplete. OrfB3, OrfB4 and OrfB5 coding for the complementary component (Acd221β) to acidocin LF221 B, the active acidocin LF221 B (Acd221B) and the putative immunity protein, respectively, are organized in one operon. Analyses of the genetic determinants of the acidocin LF221 A and acidocin LF221 B and their flanking loci revealed that LF221 acidocins are two different, two-peptide bacteriocins produced by L. gasseri LF221.
Downloads
Published
Issue
Section
License
Copyright (c) 2003 University of Ljubljana, Biotechnical Faculty

This work is licensed under a Creative Commons Attribution 4.0 International License.