Protocol used for AFLP by automatic laser fluorescence analysis

Nataša ŠTAJNER; Branka JAVORNIK; Dunja BANDELJ

doi:10.14720/aas.2003.81.1.15323

Protocol used for AFLP by automatic laser fluorescence analysis

Authors

Nataša ŠTAJNER University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia
Branka JAVORNIK University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia
Dunja BANDELJ Science and Research Centre of the Republic Slovenia, Koper, Slovenia

DOI:

https://doi.org/10.14720/aas.2003.81.1.15323

Abstract

The AFLP (amplified fragment length polymorphism) technique using fluorescence-labelled primers and automated detection is described and some features of this method are discussed. The modified AFLP technique is suitable for efficient fingerprinting analysis and gives a much higher resolution than silver staining detection systems. With the aid of an automated ALFexpress sequencer and computer software, the AFLP method is much considerably easier to perform and the total time required is reduced.

Published

15. 05. 2003

Issue

Vol. 81 No. 1 (2003): PLANT PRODUCTION

Section

Original Scientific Article

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

ŠTAJNER, N., JAVORNIK, B., & BANDELJ, D. (2003). Protocol used for AFLP by automatic laser fluorescence analysis. Acta Agriculturae Slovenica, 81(1), 39–45. https://doi.org/10.14720/aas.2003.81.1.15323