Microsatellite analysis by automated fluorescent detection using ALFexpress aparatus: A case of grapevine analysis

Petra KOZJAK; Zora KOROŠEC-KORUZA; Branka JAVORNIK

doi:10.14720/aas.2003.81.1.15324

Microsatellite analysis by automated fluorescent detection using ALFexpress aparatus: A case of grapevine analysis

Authors

Petra KOZJAK University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia
Zora KOROŠEC-KORUZA University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia
Branka JAVORNIK University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia

DOI:

https://doi.org/10.14720/aas.2003.81.1.15324

Abstract

The amplification of twenty-three microsatellite loci was optimised for automatic fluorescent detection using an ALFexpress aparatus for genotyping grapevine. By optimising PCR reaction mixture and PCR condition, the generation of fragments was found to be locus specific. Problems in allele calling and sizing due to generation of additional fragments, intergel variability and allele microvariability, are also discussed.

Published

15. 05. 2003

Issue

Vol. 81 No. 1 (2003): PLANT PRODUCTION

Section

Original Scientific Article

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

KOZJAK, P., KOROŠEC-KORUZA, Z., & JAVORNIK, B. (2003). Microsatellite analysis by automated fluorescent detection using ALFexpress aparatus: A case of grapevine analysis. Acta Agriculturae Slovenica, 81(1), 47–55. https://doi.org/10.14720/aas.2003.81.1.15324