Vpliv priprave DNK na občutljivost odkrivanja bakterij Listeria monocytogenes s PCR

Anja KLANČNIK; Sonja SMOLE MOŽINA; Barbara JERŠEK

doi:10.14720/aas.2003.81.1.15326

The effect of DNA preparation procedure on sensitivity of PCR detection of Listeria monocytogenes

Authors

Anja KLANČNIK University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia
Sonja SMOLE MOŽINA University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia
Barbara JERŠEK University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia

DOI:

https://doi.org/10.14720/aas.2003.81.1.15326

Abstract

We studied the effect of DNA preparation methods of Listeria monocytogenes DNA on PCR sensitivity for L. monocytogenes detection with specific primers. We tested eight lysis protocols for releasing of DNA with detergents, enzymes and/or high temperatures. Considering PCR detection limit in a range from 10⁵ to 10⁶ cfu/ml, rapidity, low cost and preparation simplicity, we chose lysis solution consisting of 0.05 M NaOH and 0.125 % SDS to be used in the optimal procedure for L. monocytogenes DNA preparation.

Published

15. 05. 2003

Issue

Vol. 81 No. 1 (2003): PLANT PRODUCTION

Section

Original Scientific Article

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

KLANČNIK, A., SMOLE MOŽINA, S., & JERŠEK, B. (2003). The effect of DNA preparation procedure on sensitivity of PCR detection of Listeria monocytogenes. Acta Agriculturae Slovenica, 81(1), 65–73. https://doi.org/10.14720/aas.2003.81.1.15326