Extraction of chromium from yeast biomass

Authors

  • Maja PAŠ Univerza v Ljubljani, Biotehniška fakulteta, Oddelek za živilstvo, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenija
  • Radmila MILAČIČ Institut Jožef Stefan, Odsek za znanosti o okolju, Jamova 39, SI-1000 Ljubljana, Slovenija
  • Peter RASPOR Univerza v Ljubljani, Biotehniška fakulteta, Oddelek za živilstvo, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenija

DOI:

https://doi.org/10.14720/aas.2008.91.1.15393

Keywords:

yeasts, chromium, extraction

Abstract

Total chromium in yeast biomass does not indicate well the amount of organically bound or biologically active chromium. The study presented in this paper investigated different reagents for extraction of organically bound chromium from yeast cells and yeast protoplasts and different parameters of extraction procedure. Yeasts Candida intermedia ZIM 156 were cultivated for 12 or 22 hours at 28 C in chemically defined medium containing 1mM CrCl3 or Na2Cr2O7 (20 ?M Cr6+). Washed yeast cells were resuspended in appropriate reagents for extraction (0.05 M EDTA, 0.7 M CH3COONa, 0.1 M NH3, 0.1 M Na4P2O7.10H2O) and suspensions were incubated at 28 C. Extraction time, mixing conditions and concentration of yeast suspension in EDTA were optimized. An aliquot of yeast cells was used to prepare protoplasts from which chromium was extracted. Chromium content in extracts, in yeast biomass and in protoplasts was analysed by atomic absorption spectrometry. On the basis of our results EDTA was chosen as the most appropriate reagent for extraction, optimal extraction time was 21 hours without shaking. Furthermore, the results obtained for both chromium compounds showed, that the amounts of extracted chromium from yeast cells and from protoplasts were approximately the same. Nevertheless, we can not conclude, whether chromium, which was extracted from yeast protoplasts originated from cell interior or from yeast cell membranes. In further research exact properties of EDTA extracts should be determined and chromium compounds in the extracts should be identified.

Published

15. 05. 2008

Issue

Section

Original Scientific Article

How to Cite

PAŠ, M., MILAČIČ, R., & RASPOR, P. (2008). Extraction of chromium from yeast biomass. Acta Agriculturae Slovenica, 91(1), 239–250. https://doi.org/10.14720/aas.2008.91.1.15393

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