Amplification of fluorescent-labelled microsatellite markers in olives by a novel, economic method

Dunja BANDELJ; Jernej JAKŠE; Branka JAVORNIK

doi:10.14720/aas.2004.83.2.15445

Amplification of fluorescent-labelled microsatellite markers in olives by a novel, economic method

Authors

Dunja BANDELJ SI-5000 Koper, Garibaldijeva 18, Slovenia
Jernej JAKŠE University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, Slovenia
Branka JAVORNIK University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, Slovenia

DOI:

https://doi.org/10.14720/aas.2004.83.2.15445

Keywords:

microsatellite, fluorescent detection, universal M13(-21) primer, olive

Abstract

A novel method for the economic fluorescent labelling of PCR products and its features are described. To investigate the efficiency of the method for amplification of olive microsatellite markers, PCR conditions of three loci were optimised. The results showed that the method can be successfully applied in genotyping studies of olive varieties.

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Published

15. 11. 2004

Issue

Vol. 83 No. 2 (2004): PLANT PRODUCTION

Section

Original Scientific Article

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

BANDELJ, D., JAKŠE, J., & JAVORNIK, B. (2004). Amplification of fluorescent-labelled microsatellite markers in olives by a novel, economic method. Acta Agriculturae Slovenica, 83(2), 323–329. https://doi.org/10.14720/aas.2004.83.2.15445