Analiza sekvenc genov za 16S rRNA izolatov bifidobakterij iz tankega črevesa podgan

Lijana FANEDL; Franc Viktor NEKREP; Gorazd AVGUŠTIN

doi:10.14720/aas.2002.80.2.15577

Authors

Lijana FANEDL Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia
Franc Viktor NEKREP Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia
Gorazd AVGUŠTIN Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia

DOI:

https://doi.org/10.14720/aas.2002.80.2.15577

Keywords:

microbiology, bacteria, Bifidobacterium, molecular genetics, 16S rDNA, sequences, phylogeny, taxonomy, rats, small intestine

Abstract

The amplified 16S rRNA genes from 6 isolates of bifidobacteria from rat small intestine and two isolates of lactobacilli were directly sequenced. A phylogenetic tree was constructed using the determined sequences and sequences of related genera from DNA databases. All bifidobacterial isolates belonged to the B. lactis group and formed two subgroups. The similarity levels between 16S rRNA sequences of isolates ranged from 91.4 to 95.8 %, showing great genetic variability among isolates. With similarity levels from 95 to 97 %, the phylogenetic analysis also showed that sequences of our isolates were closely related to B. pseudolongum. The results of previous phenotypic and genotypic studies and results obtained from the phylogenetic analysis of 16S rRNA sequences form the foundation for further analysis to confirm that our isolates represent one or more new bifidobacterial species.