16S rRNA analysis of bifidobacterial isolates from rat small intestine
DOI:
https://doi.org/10.14720/aas.2002.80.2.15577Keywords:
microbiology, bacteria, Bifidobacterium, molecular genetics, 16S rDNA, sequences, phylogeny, taxonomy, rats, small intestineAbstract
The amplified 16S rRNA genes from 6 isolates of bifidobacteria from rat small intestine and two isolates of lactobacilli were directly sequenced. A phylogenetic tree was constructed using the determined sequences and sequences of related genera from DNA databases. All bifidobacterial isolates belonged to the B. lactis group and formed two subgroups. The similarity levels between 16S rRNA sequences of isolates ranged from 91.4 to 95.8 %, showing great genetic variability among isolates. With similarity levels from 95 to 97 %, the phylogenetic analysis also showed that sequences of our isolates were closely related to B. pseudolongum. The results of previous phenotypic and genotypic studies and results obtained from the phylogenetic analysis of 16S rRNA sequences form the foundation for further analysis to confirm that our isolates represent one or more new bifidobacterial species.
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Copyright (c) 2002 University of Ljubljana, Biotechnical Faculty
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