Opredelitev populacij kranjske čebele (Apis mellifera carnica Pollman) na osnovi analize mitohondrijske DNK

Peter KOZMUS; Simona SUŠNIK; Janez POKLUKAR; Vladimir MEGLIČ

doi:10.14720/aas.2002.80.2.15579

Authors

Peter KOZMUS Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia
Simona SUŠNIK Agricultural Institute of Slovenia, Hacquetova 17, SI-1000 Ljubljana, Slovenia
Janez POKLUKAR Agricultural Institute of Slovenia, Hacquetova 17, SI-1000 Ljubljana, Slovenia
Vladimir MEGLIČ Agricultural Institute of Slovenia, Hacquetova 17, SI-1000 Ljubljana, Slovenia

DOI:

https://doi.org/10.14720/aas.2002.80.2.15579

Keywords:

bees, Carniolan bee, Apis mellifera carnica, molecular genetics, mitochondrial DNA, COI-II. region, Slovenia

Abstract

According to morphology, the carniolan bee (Apis mellifera carnica Pollman) is placed in South-Eastern European honey-bee group. All honey-bee subspecies are with regard to morphology and mtDNA variability divided into three main phylogenetic lineages. A. m. carnica and C phylogenetic lineage as a whole, is genetically poorly studied, therefore, carniolan bee has been characterised on the basis of mtDNA polymorphism. Fifty-six samples from Slovenia and 55 samples from other countries and selected lines were surveyed. Variable intergenic region between cytochrome-oxidase I (COI) and cytochrome-oxidase II (COII) in mtDNA was amplified. Polymorphism search was based on RFLP profiles and sequence analysis of this region. Five different haplotypes were determined, four of them being of C phylogenetic lineage origin. According to the results we can conclude that except in some selected lines, all populations analysed, including Slovenian ones, were not affected by introduction of non-native queen honey-bees.