Dokazovanje protiteles proti patogenim mikroorganizmom v tekočinah kokošjega zarodka

Darja HERMAN; Mojca NARAT; Dušan BENČINA

doi:10.14720/aas.2001.78.2.15592

Authors

Darja HERMAN Univ.v Ljubljani, Biotehniška fak., Odd. za zootehniko, Groblje 3, SI-1230 Domžale in sedaj Univ. v Ljubljani, Medicinska fak., Inštitut za biokemijo in molekularno biologijo, Lipičeva 2, SI-1000, Ljubljana, Slovenija
Mojca NARAT Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia
Dušan BENČINA Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia

DOI:

https://doi.org/10.14720/aas.2001.78.2.15592

Keywords:

poultry, chicken embryo, immunology, immunoglobulins, allantoic fluid, amniotic fluid, bile

Abstract

Certain avian pathogens can be transmitted in chickens via hatching eggs. Specific maternal antibodies appearing in the egg yolk and egg white are able to protect embryo against a variety of vertically transmitted bacterial and viral pathogens. Immunoenzyme tests using different monoclonal antibodies (mAb) to chicken immunoglobulins G (IgG), IgA and IgM were used for detection of antibodies to Mycoplasma synoviae, M. gallisepticum, Ornithobacterium rhinotracheale and Newcastle disease virus in the allantoic fluid (ALF), amniotic fluid (AMF), bile and serum of chicken embryos on different stages of the embryonic development. In the ALF of 7-day-old embryos IgG antibodies to M. synoviae were demonstrated for the first time in this study. Certain samples contained also IgG antibodies to M. gallisepticum and Newcastle disease virus. In addition, IgA, and IgM were also found in the ALF. It seems that transfer of IgA and IgM from the egg white to ALF is selective. In AMF specific IgG antibodies to M. synoviae were detected on day 7 of the embrionic development. From 12^th day of the embryogenesis onward levels of IgA, IgG and IgM in AMF were apparently increased. Specific IgA and IgG antibodies to M. synoviae and O. rhinotracheale were detected in bile of 13-day-old embryos. In the blood, which was taken from the chorioallantoic veins from the 13^th to 16^th day of the embryogenesis, IgG and IgA were found.